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Klein Dieter,

** = Publications listed in SCI/SSCI/Pubmed

2019

Journal Article

** Rushton, JG; Ertl, R; Klein, D; Tichy, A; Nell, B (2019): Circulating cell-free DNA does not harbour a diagnostic benefit in cats with feline diffuse iris melanomas. J Feline Med Surg 21(2): 124-132.

2017

** Rushton, JG; Ertl, R; Klein, D; Nell, B (2017): Mutation analysis and gene expression profiling of ocular melanomas in cats. Vet Comp Oncol. 2017; 15(4):1403-1416

2015

** Ertl, R; Korb, M; Langbein-Detsch, I; Klein, D (2015): Prevalence and risk factors of gammaherpesvirus infection in domestic cats in Central Europe. Virol J. 2015; 12:146
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** Krunic, M; Ertl, R; Hagen, B; Sedlazeck, FJ; Hofmann-Lehmann, R; von Haeseler, A; Klein, D (2015): Decreased expression of endogenous feline leukemia virus in cat lymphomas: a case control study. BMC Vet Res. 2015; 11:90
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** Metzler-Zebeli, BU; Ertl, R; Klein, D; Zebeli, Q (2015): Explorative study of metabolic adaptations to various dietary calcium intakes and cereal sources on serum metabolome and hepatic gene expression in juvenile pigs. Metabolomics (11), 3 545-558.

** Metzler-Zebeli, BU; Mann, E; Ertl, R; Schmitz-Esser, S; Wagner, M; Klein, D; Ritzmann, M; Zebeli, Q (2015): Dietary calcium concentration and cereals differentially affect mineral balance and tight junction proteins expression in jejunum of weaned pigs. Br J Nutr. 2015; 113(7):1019-1031

2014

** Burgstaller, JP; Johnston, IG; Jones, NS; Albrechtová, J; Kolbe, T; Vogl, C; Futschik, A; Mayrhofer, C; Klein, D; Sabitzer, S; Blattner, M; Gülly, C; Poulton, J; Rülicke, T; Piálek, J [and 2 others] (2014): MtDNA segregation in heteroplasmic tissues is common in vivo and modulated by haplotype differences and developmental stage. Cell Rep. 2014; 7(6):2031-2041
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** Ertl, R; Klein, D (2014): Transcriptional profiling of the host cell response to feline immunodeficiency virus infection. Virol J. 2014; 11:52
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** Pajer, K; Feichtinger, GA; Márton, G; Sabitzer, S; Klein, D; Redl, H; Nógrádi, A (2014): Cytokine signaling by grafted neuroectodermal stem cells rescues motoneurons destined to die. Exp Neurol. 2014; 261:180-189

2013

** Metzler-Zebeli, BU; Hollmann, M; Sabitzer, S; Podstatzky-Lichtenstein, L; Klein, D; Zebeli, Q (2013): Epithelial response to high-grain diets involves alteration in nutrient transporters and Na+/K+-ATPase mRNA expression in rumen and colon of goats. J Anim Sci. 2013; 91(9):4256-4266

** Schäfer-Somi, S; Sabitzer, S; Klein, D; Reinbacher, E; Kanca, H; Beceriklisoy, HB; Aksoy, OA; Kucukaslan, I; Macun, HC; Aslan, S (2013): Vascular endothelial (VEGF) and epithelial growth factor (EGF) as well as platelet-activating factor (PAF) and receptors are expressed in the early pregnant canine uterus. Reprod Domest Anim. 2013; 48(1):20-26

** Watkins, JD; Sholukh, AM; Mukhtar, MM; Siddappa, NB; Lakhashe, SK; Kim, M; Reinherz, EL; Gupta, S; Forthal, DN; Sattentau, QJ; Villinger, F; Corti, D; Ruprecht, RM (2013): Anti-HIV IgA isotypes: differential virion capture and inhibition of transcytosis are linked to prevention of mucosal R5 SHIV transmission. AIDS. 2013; 27(9):F13-F20
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2012

** Hartmann, K; Stengel, C; Klein, D; Egberink, H; Balzarini, J (2012): Efficacy and adverse effects of the antiviral compound plerixafor in feline immunodeficiency virus-infected cats. J Vet Intern Med. 2012; 26(3):483-490

** Justa, N; Weber, K; Klein, D; Mueller, RS; Sauter-Louis, C; Hartmann, K (2012): Efficacy and adverse effects of (R)-9-(2-phosphonylmethoxypropyl)-2,6-diaminopurine in feline immunodeficiency virus-infected cats. J Vet Intern Med. 2012; 26(6):1267-1273

** Schäfer-Somi, S; Sabitzer, S; Klein, D; Tomaszewski, C; Kanca, H; Beceriklisoy, HB; Kucukaslan, I; Agaoglu, R; Kaya, D; Ay, S; Aslan, S (2012): Is apoptosis a regulatory mechanism during early canine pregnancy? Reprod Domest Anim. 2012; 47 Suppl 6:169-172

Conference Paper in Journal

** Schäfer-Somi, S; Sabitzer, S; Klein, D; Reinbacher, E; Kanca, H; Beceriklisoy, HB; Kucukaslan, I; Kaya, D; Macun, HC; Aslan, S (2012): Vascular Endothelial (VEGF) and Epidermal Growth Factor (EGF) as well as Platelet Activating Factor (PAF) and receptors are expressed in the early pregnant canine uterus. 45th Annual Conference of Physiology and Pathology of Reproduction/37th Joint Conference on Veterinary and Human Reproduction Medicine/1st Joint German-Polish Conference on Reproductive Medicine, Berlin, GERMANY, FEB 29 - MAR 02, 2012. Reprod Domest Anim (47), S2 46-47.

Published (citable) presentations at scientific conferences (A2)

Ertl, D; Ertl, R; Petrovic, S; Klein, K; Krunic, M; von Haeseler, A; Klein, D (2012): Transcriptome and pathway analysis of feline lymphoma using RNA-seq technology. -11th International Feline Retrovirus Research Symposium; AUG 26-29, 2012; Leipzig, GERMANY.

Ertl, R; Philippi-Novak, T; Niedermayer, C; Stücklschwaiger, D; Klein, D (2012): Cellular transcriptome analysis of feline immunodeficiency virus infected cells using next-generation sequencing technology. -11th International Feline Retrovirus Research Symposium; AUG 26-29, 2012; Leipzig, GERMANY.

Hagen, B; Ertl, R; Krunic, M; Tauber, S; Hofmann-Lehmann, R; von Haeseler, A; Klein, D (2012): Analysis of retroviral gene expression in feline lymphoma using qPCR and next-generation-sequencing technology. -11th International Feline Retrovirus Research Symposium; AUG 26-29, 2012; Leipzig, GERMANY.

Klein, D (2012): Feline Immunodeficiency Virus as a natural animal model for HIV vaccine research: Advantages and limitations. 57-57.-BIT’s 4th Annual World Vaccine Congress; MAR 26-28, 2012; Beijing, CHINA.

Sabitzer, S; Schneider, M; Grdseloff, N; Wolfesberger, B; Walter, I; Klein, D (2012): Comparative gene expression profiling of feline lymphomas. -11th International Feline Retrovirus Research Symposium; AUG 26-29, 2012; Leipzig, GERMANY.

Schäfer-Somi, S; Sabitzer, S; Klein, D; Kanca, H; Beceriklisoy, HB; Kucukaslan, I; Kaya, D; Ceyhun, M; Aslan, S (2012): Is apoptosis a regulatory mechanism during early canine pregnancy?. 234-235.-International Symposium on Canine and Feline Reproduction (ISCFR); JUL 26-29, 2012; Whistler, Vancouver, CANADA.

Schwarz, B; Ertl, R; Zimmer, S; Netzmann, Y; Klein, D; Schwendenwein, I; Van den Hoven, R (2012): Hilft ein submaximaler Belastungstest Haflinger mit und ohne GYS-1 Mutation (PSSM Typ 1) zu unterscheiden?. 81-81.-22. Arbeitstagung der DVG-Fachgruppe Pferdekrankheiten; MAR 16-17, 2012; Hannover. (ISBN: 978-3-86345-064-9 )

2011

Journal Article

** Ertl, R; Birzele, F; Hildebrandt, T; Klein, D (2011): Viral transcriptome analysis of feline immunodeficiency virus infected cells using second generation sequencing technology. 10th International Feline Retrovirus Research Symposium (IFRRS), Charleston, SC, MAY 23-26, 2010. Vet Immunol Immunopathol. 2011; 143(3-4):314-324

** Jais, A; Klein, D; Wolfesberger, B; Walter, I (2011): Gene expression profile of vascular endothelial growth factor (VEGF) and its receptors in various cell types of the canine lymph node using laser capture microdissection (LCM). Vet Immunol Immunopathol. 2011; 140(3-4):207-214

** Schwarz, B; Ertl, R; Zimmer, S; Netzmann, Y; Klein, D; Schwendenwein, I; Hoven, RV (2011): Estimated prevalence of the GYS-1 mutation in healthy Austrian Haflingers. Vet Rec. 2011; 169(22):583

Published (citable) presentations at scientific conferences (A2)

Klein, D; Ertl, R; Birzele, F; Hildebrandt, T (2011): Feline Immunodeficiency Virus as a Natural Animal Model for HIV Research: New Insights into Virus Host Interactions using Second Generation Sequencing Technology. 254--BIT’s 3rd Annual World Vaccine Congress; MAR 23-25, 2011; Beijing, CHINA.

2010

Journal Article

** Kraase, M; Sloan, R; Klein, D; Logan, N; McMonagle, L; Biek, R; Willett, BJ; Hosie, MJ (2010): Feline immunodeficiency virus env gene evolution in experimentally infected cats. 9th International Feline Retrovirus Research Symposium, Vienna, AUSTRIA, AUG 24-27, 2008. Vet Immunol Immunopathol. 2010; 134(1-2):96-106

** Steinrigl, A; Ertl, R; Langbein, I; Klein, D (2010): Phylogenetic analysis suggests independent introduction of feline immunodeficiency virus clades A and B to Central Europe and identifies diverse variants of clade B. 9th International Feline Retrovirus Research Symposium, Vienna, AUSTRIA, AUG 24-27, 2008. Vet Immunol Immunopathol. 2010; 134(1-2):82-89

Published (citable) presentations at scientific conferences (A2)

Ertl, R; Birzele, F; Hildebrandt, T; Klein, D (2010): Analysis of virus-host interactions in Feline immunodeficiency virus infected cells using massive parallel sequencing technology. 38-38.-22nd International Workshop on Retroviral Pathogenesis; NOV 17-21, 2010; Newport Beach, USA.

Ertl, R; Birzele, F; Hildebrandt, T; Klein, D (2010): Analysis of virus-host interactions in FIV infected cells using massive parallel sequencing technology. -Illumina User Meeting ; JUL 13-15, 2010; Barcelona.

Ertl, R; Birzele, F; Hildebrandt, T; Klein, D (2010): Analysis of virus-host interactions in FIV infected cells using massive parallel sequencing technology. 48--32. Jahrestagung der ÖGHMP ; MAY 17-20, 2010; Wien.

Ertl, R; Birzele, F; Hildebrandt, T; Klein, D (2010): Analysis of virus-host interactions in FIV infected cells using massive parallel sequencing technology. 56--10th International Feline Retrovirus Research Symposium ; May 23-26, 2010; Charleston, USA.

Klein, D (2010): Feline Immunschwächeviren: Machen sie Katzen überhaupt krank?. 9-11.-AfT-Herbstsymposium – Virale Infektionskrankheiten bei der Katze; NOV 6, 2010; Munich, GERMANY.

Rütgen, B; Sabitzer, S; Wolfesberger, B; Kleiter, M; Klein, D; Walter, I; Schwendenwein, I; Saalmüller, A; Hammer, SE (2010): Characterization of the canine B-cell line CLBL-1 in comparison with other canine cell lines using Gene Expression Profiling. -Annual VCS-Conference; OCT 29-NOV 1, 2010; San Diego, CA (USA).

Schäfer-Somi, S; Reinbacher, E; Sabitzer, S; Klein, D; Kanca, H; Aksoy, OA; Kucukaslan, I; Macun, HC; Aslan, S (2010): Growth and angiogenesis in the pregnant canine uterus. -4th International Symposium on non-surgical contraceptive methods of pet population control; APR 8-10, 2010; Dallas, Texas, USA.

2009

Journal Article

** Klonjkowski, B; Klein, D; Galea, S; Gavard, F; Monteil, M; Duarte, L; Fournier, A; Sayon, S; Górna, K; Ertl, R; Cordonnier, N; Sonigo, P; Eloit, M; Richardson, J (2009): Gag-specific immune enhancement of lentiviral infection after vaccination with an adenoviral vector in an animal model of AIDS. Vaccine. 2009; 27(6):928-939

** Paar, M; Klein, D; Salmons, B; Günzburg, WH; Renner, M; Portsmouth, D (2009): Influence of vector design and host cell on the mechanism of recombination and emergence of mutant subpopulations of replicating retroviral vectors. BMC Mol Biol. 2009; 10:8
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** Schäfer-Somi, S; Beceriklisoy, HB; Walter, I; Sabitzer, S; Klein, D; Kanca, H; Kaya, D; Agaoglu, AR; Izgür, H; Aslan, S (2009): Expression of MHC-I and -II in uterine tissue from early pregnant bitches. 6th International Symposium on Canine and Feline Reproduction, Vienna, AUSTRIA, JUL 09-11, 2008. Reprod Domest Anim. 2009; 44 Suppl 2:103-108

** Schäfer-Somi, S; Klein, D; Beceriklisoy, HB; Sabitzer, S; Ay, SS; Agaoglu, AR; Kücükaslan, I; Kaya, D; Aksoy, OA; Aslan, S (2009): Uterine progesterone receptor and leukaemia inhibitory factor mRNA expression in canine pregnancy. 6th International Symposium on Canine and Feline Reproduction, Vienna, AUSTRIA, JUL 09-11, 2008. Reprod Domest Anim. 2009; 44 Suppl 2:109-114

Conference Paper in Journal

** Hlavaty, J; Petznek, H; Konig-Schuster, M; Sabitzer, S; Klein, D; Salmons, B; Gunzburg, W; Egerbacher, M; Hohenadl, C; Renner, M (2009): Brain tumors as a target for MLV-based replication-competent retroviral vector-mediated suicide gene therapy. Combined Meeting of the 17th European-Society-of-Gene-and-Cell-Therapy/16th German-Society-for-Gene-Therapy/4th German-Society-for-Stem-Cell-Research, Hannover, GERMANY, NOV 21-25, 2009. Hum Gene Ther (20), 11 1444-1444.

Published (citable) presentations at scientific conferences (A2)

Sand, C; Stengel, C; Klein, D; Sauter-Louis, C; Hartmann, K (2009): Association of provirus and virus load with the health status of cats naturally infected with feline immunodeficiency virus. 225-225.-19th Annual Congress of the European College of Veterinary Internal Medicine – Companion Animals (ECVIM-CA); SEP 8-10, 2009; Porto, PORTUGAL.

2008

Conference Paper in Journal

** Budik, S; Klein, D; Knapp, E; Pittner, F; Aurich, C (2008): Cryopreservation of stallion semen has no negative effect on size, quality or expression of viability markers in early equine embryos. 5th International Symposium on Stallion Reproduction , Gramado, Rio Grande do Sul, Brazil, September 18-20, 2008. Anim Reprod Sci (107), 3-4 311-311.

Published (citable) presentations at scientific conferences (A2)

Hofer, M; Klein, D; Steinrigl, A (2008): Establishment and validation of real-time PCR assays for the quantification of different forms of Feline Immunodeficiency Virus DNA. 71--9th International Feline Retrovirus Research Symposium; AUG 24-27, 2008; Vienna, AUSTRIA.

Kraase, M; Sloan, R; Klein, D; Willett, BJ; Hosie, MJ (2008): Feline Immunodeficiency Virus env gene quasispecies evolution in experimentally infected cats. 63--9th International Feline Retrovirus Research Symposium; AUG 24-27, 2008; Vienna, AUSTRIA.

Schäfer-Somi, S; Beceriklisoy, HB; Walter, I; Sabitzer, S; Klein, D; Kanca, H; Kaya, D; Agaoglu, AR; Izgür, H; Aslan, S (2008): Expression of MHC-I and -II in uterine tissue from early pregnant bitches. 218-219.-6th International Symposium on Canine and Feline Reproduction and 6th Biannual EVSSAR Meeting; JUL 9-11, 2008; Vienna, AUSTRIA.

Steinrigl, A; Ertl, R; Hofer, M; Klein, D (2008): Expression of extrachromosomal DNA is inefficient in Murine Leukemia Virus. 65--9th International Feline Retrovirus Research Symposium; AUG 24-27, 2008; Vienna, AUSTRIA.

Steinrigl, A; Ertl, R; Hofer, M; Klein, D (2008): Inefficient expression of extrachromosomal DNA in Murine Leukemia Virus. 82-82.-20th International Workshop on Retroviral Pathogenesis; NOV 13-16, 2008; Toronto, CANADA.

Steinrigl, A; Ertl, R; Knapp, E; Langbein, I; Klein, D (2008): Phylogenetic analysis of European FIV-sequences suggests independent introduction of subtypes A and B to Central Europe. 17--9th International Feline Retrovirus Research Symposium; AUG 24-27, 2008; Vienna, AUSTRIA.

2007

Journal Article

** Kolm, G; Knapp, E; Wagner, R; Klein, D (2007): Lactoferrin, a glycoprotein with immunomodulatory and mast cell stabilising properties, in skin of horses suffering from Culicoides hypersensitivity. Res Vet Sci. 2007; 83(2):165-170

** Steinrigl, A; Nosek, D; Ertl, R; Günzburg, WH; Salmons, B; Klein, D (2007): Mutations in the catalytic core or the C-terminus of murine leukemia virus (MLV) integrase disrupt virion infectivity and exert diverse effects on reverse transcription. Virology. 2007; 362(1):50-59
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Conference Paper in Journal

Steinrigl, A; Ertl, R; Gansterer, M; Günzburg, WH; Salmons, B; Klein, D (2007): Influence of producer cell DNA carryover on retroviral titer and transduction efficiency estimation. 15th Annual Congress of the European Society of Gene and Cell Therapy, Rotterdam, NETHERLANDS, OCT 27-30, 2007. Hum Gene Ther (18), 10 1036.

Published (citable) presentations at scientific conferences (A2)

Schild, M; Salmons, B; Günzburg, WH; Klein, D; Steinrigl, A (2007): Significance of the central polypurine tract for feline immunodeficiency virus transduction efficiency. 52--19th International Workshop on Retroviral Pathogenesis; SEP 25-29, 2007; Vienna, AUSTRIA.

Steinrigl, A; Ertl, R; Klein, D (2007): Detailed investigation of the retroviral life cycle using real-time PCR assays. 46-46.-3rd International qPCR Symposium; 26.-28.03.2007; Freising.

Steinrigl, A; Ertl, R; Salmons, B; Günzburg, WH; Klein, D (2007): Comparison between HIV-1 and MoMLV integrase mutants: differences regarding the abundance of 2-LTR circles and expression from extrachromosomal DNA. 176-176.-3rd European Congress of Virology; 01.-05.09.2007; Nürnberg.

Steinrigl, A; Ertl, R; Salmons, B; Günzburg, WH; Klein, D (2007): Estimation of retroviral titre and transduction efficiency by real-time PCR is easily biased by DNA-carryover from transient producer cells. 169-169.-3rd European Congress of Virology; 01.-05.09.2007; Nürnberg.

Steinrigl, A; Nosek, D; Ertl, R; Salmons, B; Günzburg, WH; Klein, D (2007): Moloney Murine leukemia virus integrase mutants suggest differences to lentiviruses in the role of extrachromosomal DNA for the viral life cycle. 53--19th International Workshop on Retroviral Pathogenesis; SEP 25-29, 2007; Vienna, AUSTRIA.

2006

Journal Article

** Dunham, SP; Bruce, J; Klein, D; Flynn, JN; Golder, MC; MacDonald, S; Jarrett, O; Neil, JC (2006): Prime-boost vaccination using DNA and whole inactivated virus vaccines provides limited protection against virulent feline immunodeficiency virus. Vaccine. 2006; 24(49-50):7095-7108

** Kolm, G; Klein, D; Knapp, E; Watanabe, K; Walter, I (2006): Lactoferrin expression in the horse endometrium: relevance in persisting mating-induced endometritis. Vet Immunol Immunopathol. 2006; 114(1-2):159-167

** Kolm, G; Knapp, E; Wagner, R; Klein, D (2006): Increased interleukin-1beta mRNA expression in skin biopsies of horses with Culicoides hypersensitivity following challenge with Culicoides nubeculosus extract. Vet Immunol Immunopathol. 2006; 113(1-2):90-98

** Schäfer-Somi, S; Kluger, S; Knapp, E; Klein, D; Aurich, C (2006): Effects of semen extender and semen processing on motility and viability of frozen-thawed dog spermatozoa. Theriogenology. 2006; 66(2):173-182

Conference Paper in Journal

** Budik, S; Klein, D; Knapp, E; Pittner, F; Aurich, C (2006): Effects of progesterone treatment during the luteal phase on quantitative gene expression in equine embryos on day 10 post ovulation. Ninth International Symposium on Equine Reproduction - Equine Reproduction IX, Kerkrade, The Netherlands, 04 August 2006. Animal Reproduction Science (94), 1-4 366-369 .

Published (citable) presentations at scientific conferences (A2)

Budik, S; Klein, D; Knapp, E; Pittner, F; Aurich, C (2006): Effects of progesterone treatment during the luteal phase on quantitative gene expression in equine embryos on day 10 post ovulation. Equine Reproduction IX, Kerkrade, The Netherlands, 6.-11. Aug. 2006. Equine Reproduction IX, Kerkrade, The Netherlands, 6.-11. Aug. 2006.

Salmons, B; Hlavaty, J; Stracke, A; Portsmouth, D; Klein, D; Klein, R; Öztürk-Winder, F; Brandtner, E-M; Tabotta, W; Indik, S; Faschinger, A; Knapp, E; Renner, M; Hohenadl, C; Rouault, F; Günzburg, W.H (2006): MLV and MMTV-based retroviral vectors for safe, efficient and targeted gene therapy: a comprehensive approach. International Society for Cell and Gene Therapy of Cancer, Shenzhen, China, 9.-11.12.05 . International Society for Cell and Gene Therapy of Cancer, Shenzhen, China, 9.-11.12.05 .

Steinrigl, A; Quintino, L; Pusch, M; Ertl, R; Klein, D (2006): Factors involved in infection of non-dividing cells by feline immunodeficiency virus. 7th International Congress of Veterinary Virology, Lissabon, 24.-27.09.2006. 7th International Congress of Veterinary Virology, Lissabon, 24.-27.09.2006.

Steinrigl, A; Quintino, L; Pusch, M; Ertl, R; Klein, D (2006): Factors involved in infection of non-dividing cells by feline immunodeficiency virus. 8th International Feline Retrovirus Research Symposium, Washington, 8.-11.10.2006. 8th International Feline Retrovirus Research Symposium, Washington, 8.-11.10.2006.

Steinrigl, A; Salmons, B; Günzburg, W.H; Klein, D (2006): Inhibition of the retroviral integration machinery as a first step towards targeted integration using chimeric viral vectors. 30. Jahrestagung ÖGHMP, Linz, 29.05.-01.06.2006. 30. Jahrestagung ÖGHMP, Linz, 29.05.-01.06.2006.

Steinrigl, A; Salmons, B; Günzburg, W.H; Klein, D (2006): Retroviral vectors harbouring elements from adeno-associated virus to facilitate site-specific integration. XIV Annual Congress of the European Society of Gene Therapy, Athen, 9.-12.11.2006. XIV Annual Congress of the European Society of Gene Therapy, Athen, 9.-12.11.2006.

Steinrigl, A; Salmons, B; Günzburg, W.H; Klein, D (2006): Inhibition of the retroviral integration machinery as a first step towards targeted integration using chimeric viral vectors. Jahrestagung der Gesellschaft für Virologie, München, 15.-18.03.2006. Jahrestagung der Gesellschaft für Virologie, München, 15.-18.03.2006.

2005

Journal Article

** de Andrés, D; Klein, D; Watt, NJ; Berriatua, E; Torsteinsdottir, S; Blacklaws, BA; Harkiss, GD (2005): Diagnostic tests for small ruminant lentiviruses. Vet Microbiol. 2005; 107(1-2):49-62

** Hosie, MJ; Klein, D; Binley, JM; Dunsford, TH; Jarrett, O; Neil, JC; Knapp, E; Giannecchini, S; Matteucci, D; Bendinelli, M; Hoxie, JA; Willett, BJ (2005): Vaccination with an inactivated virulent feline immunodeficiency virus engineered to express high levels of Env. J Virol. 2005; 79(3):1954-1957
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** Indik, S; Schmoll, F; Sipos, W; Klein, D (2005): Genetic variability of PRRS virus in Austria: consequences for molecular diagnostics and viral quantification. Vet Microbiol. 2005; 107(3-4):171-178

Conference Paper in Journal

Schäfer-Somi, S; Knapp, E; Kluger, S; Klein, D; Aurich, C (2005): The influence of different freezing and thawing techniques on motility and membrane integrity of frozen-thawed dog spermatozoa. Schweizer Archiv für Tierheilkunde 147,(2).

Published (citable) presentations at scientific conferences (A2)

Aurich, C; Klein, D; Pittner, F; Budik, S (2005): Effects of progesterone treatment on quantitative gene expression in the early equine embryo. 21st Annual Meeting A.E.T.E., Keszthely, Ungarn, 9-10th Sept. 2005. 21st Annual Meeting A.E.T.E., Keszthely, Ungarn, 9-10th Sept. 2005.

Klein, D; Pusch, M; Steinrigl, A; Knapp, E; Müller, E; Hübner, J; Langbein, I (2005): Felines Immunschwächevirus: Molekular-epidemiologische Untersuchungen und deren Relevanz für molekulare Diagnostik und Therapiestudien. 51. Jahrestagung der Deutschen Gesellschaft für Kleintiermedizin, Berlin, 17.-20.11.2005. 51. Jahrestagung der Deutschen Gesellschaft für Kleintiermedizin, Berlin, 17.-20.11.2005.

Klein, D; Quitino, L; Pusch, M; Steinrigl, A (2005): Factors involved in infection of non-dividing cells by feline immunodeficiency virus. Jahrestagung der Gesellschaft für Virologie 2005, Hannover, 16.-19.03.2005. Jahrestagung der Gesellschaft für Virologie 2005, Hannover, 16.-19.03.2005.

Steinrigl, A; Knapp, E; Salmons, B; Günzburg W.H; Klein, D (2005): Inhibition of the retroviral integration machinery as a first step towards targeted integration using chimeric viral vectors. XXII Symposium of the International Association for Comparative Research on Leukemia and Related Diseases, Heidelberg, 02.-05.07.2005. XXII Symposium of the International Association for Comparative Research on Leukemia and Related Diseases, Heidelberg, 02.-05.07.2005.

Steinrigl, A; Quitino, L; Pusch, M; Knapp, E; Klein, D (2005): Factors involved in infection of non-dividing cells by feline immunodeficiency virus. Cold Spring Harbor Laboratory Retrovirus Symposium 2005, Cold Spring Harbor, 24.-29.05.2005. Cold Spring Harbor Laboratory Retrovirus Symposium 2005, Cold Spring Harbor, 24.-29.05.2005.

2004

Journal Article

** Blacklaws, BA; Berriatua, E; Torsteinsdottir, S; Watt, NJ; de Andres, D; Klein, D; Harkiss, GD (2004): Transmission of small ruminant lentiviruses. Vet Microbiol. 2004; 101(3):199-208

** Hlavaty, J; Stracke, A; Klein, D; Salmons, B; Günzburg, WH; Renner, M (2004): Multiple modifications allow high-titer production of retroviral vectors carrying heterologous regulatory elements. J Virol. 2004; 78(3):1384-1392
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** Huebner, J; Klein, D; Müller, E; Vahlenkamp, TW; Langbein, I (2004): Kombinierte anti-retrovirale Therapie (CART) bei einer mit dem Felinen Immunschwächevirus (FIV)-infizierten Katze. Kleintier-Praxis (49), 8 517-524.

** Zetner, K; Stoian, C; Benetka, V; Klein, D; Möstl, K (2004): Der Einfluß von rekombinantem, felinen Omega-Interferon auf die chronische Gingivostomatitis der Katze. Praktischer Tierarzt (85), 11 798-807.

Conference Paper in Journal

Huebner, J; Klein, D; Müller, E; Vahlenkamp, T.W; Langbein, I (2004): Long-Term Combined Anti-Retroviral Therapy (CART) in Feline Immunodeficiency Virus Infected Cats: A Case Report. Journal of Veterinary Internal Medicine 18,(3).

Published (citable) presentations at scientific conferences (A2)

Budik, S; Klein, D; Walter, I; Pittner, F; Aurich, C (2004): Gene expression in the early equine embryo - a search for markers of embryonic viability. Third Meeting of the European Equine Gamete Group (EEGG), Pardubice, Czech Republic, 12th-15th Oct.2003. Third Meeting of the European Equine Gamete Group (EEGG), Pardubice, Czech Republic, 12th-15th Oct.2003.

Haining, H; McMonagle, E.L; Knapp, E; Klein, D; Willett, B.J; Hosie, M.J (2004): Studies on the pathogenicity of primary feline immunodeficiency virus isoltates from different stages of infection. 7th International Feline Retrovirus Research Symposium, Pisa, 11. - 15.09.2004. 7th International Feline Retrovirus Research Symposium, Pisa, 11. - 15.09.2004.

Huebner, J; Klein, D; Müller, E; Vahlenkamp, T.W; Langbein, I (2004): Long-Term Combined Anti-Retroviral Therapy (CART) in Feline Immunodeficiency Virus Infected Cats: A Case Report. 7th International Feline Retrovirus Research Symposium, Pisa, 11. - 15.09.2004. 7th International Feline Retrovirus Research Symposium, Pisa, 11. - 15.09.2004.

Klein, D. Brunner, B; Steinrigl, A (2004): Implications of molecular evolution on viral quantification using real-time PCR based assays. 1st International qPCR Symposium, Freising, Germany, 03.-06.03.2004. 1st International qPCR Symposium, Freising, Germany, 03.-06.03.2004.

Klein, D; Leichsenring, B; Nosek, D; Knapp, E; Salmons, B; Günzburg, W.H (2004): Replication Kinetics of Retroviral Integrase Mutants and Influence of CIS-Acting Elements. 29. Jahrestagung der ÖGHMP, Bad Ischl, 24.-27.05.2004. 29. Jahrestagung der ÖGHMP, Bad Ischl, 24.-27.05.2004.

Klein, D. Nosek, D; Leichsenring, B; Knapp, E (2004): Dissection of the retroviral life cycle using real-time PCR assays. 1st International qPCR Symposium, Freising, germany, 03.-6.03.2004. 1st International qPCR Symposium, Freising, germany, 03.-6.03.2004.

Klein, D; Schmoll, F; Sipos, W; Indik, S (2004): Genetische Variabilität von PRRSV in Österreich: Konsequenzen für molekulare Diagnostik und Virusquantifizierung. 23. AVID-Tagung, Kloster Banz / Bad Staffelstein, 15.-17.09.2004. 23. AVID-Tagung, Kloster Banz / Bad Staffelstein, 15.-17.09.2004.

Klein, D; Steinrigl, A; Knapp, E; Brunner, B; Holzweber, A; Leichsenring, B; Nosek, D; Salmons, B; Günzburg, W.H (2004): Molecular epidemiology and replication kinetics of retroviruses. Inaugural Symposium of the new Christian Doppler Laboratory for Gene Therapeutic Vector Development, Wien, 22.-24.01.2004. Inaugural Symposium of the new Christian Doppler Laboratory for Gene Therapeutic Vector Development, Wien, 22.-24.01.2004.

Quintino, L; Steinrigl, A; Klein, D (2004): Function of ORF A in the context of Feline Immunodeficiency Virus vector development. 7th International Feline Retrovirus Research Symposium, Pisa, 11. - 15.09.2004. 7th International Feline Retrovirus Research Symposium, Pisa, 11. - 15.09.2004.

Ryan, G; Klein, D; Brankin, B; McQuaid, S; Hosie, M.J; Callanan, J.J (2004): Assessment of blood-brain barrier integrity within FIV infected brain using confocal microscopy. 7th International Feline Retrovirus Research Symposium, Pisa, 11. - 15.09.2004. 7th International Feline Retrovirus Research Symposium, Pisa, 11. - 15.09.2004.

Steinrigl, A; Quintino, L; Klein, D (2004): Factors involved in transduction of non-dividing cells by Feline Immunodeficiency Virus. 7th International Feline Retrovirus Research Symposium, Pisa, 11. - 15.09.2004. 7th International Feline Retrovirus Research Symposium, Pisa, 11. - 15.09.2004.

Original Article in Series

Steinrigl, A; Klein, D (2004): Impact of high genetic variability of Feline Immunodeficiency Virus on molecular detection and anti-viral strategies. IN: Recent Research Developments in Virology. Kerala, India, Transworld Research Network, pp. 13-28.

2003

Journal Article

** Ryan, G; Klein, D; Knapp, E; Hosie, MJ; Grimes, T; Mabruk, MJ; Jarrett, O; Callanan, JJ (2003): Dynamics of viral and proviral loads of feline immunodeficiency virus within the feline central nervous system during the acute phase following intravenous infection. J Virol (77), 13 7477-7485.
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** Steinrigl, A; Klein, D (2003): Phylogenetic analysis of feline immunodeficiency virus in Central Europe: a prerequisite for vaccination and molecular diagnostics. J Gen Virol. 2003; 84(Pt 5):1301-1307
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Conference Paper in Journal

Klein, D; Nosek, D; Leichsenring, B; Knapp, E; Salmons, B; Günzburg, W.H (2003): Directing the integration of retroviral vectors: a new task in vector development. International Journal of Molecular Medicine Vol. 12,(Suppl. 1).

Steinrigl, A; Klein D (2003): Phylogenetic analysis of feline immunodeficiency virus as a prerequisite for lentiviral vaccination strategies. Eighth European Workshop on Virus Evolution and Molecular Epidemiology, Leuven, Belgium, SEP 4-11, 2002. Infection, Genetics and Evolution (2), 3 219-219.

Published (citable) presentations at scientific conferences (A2)

Aurich, C; Klein, D; Pittner, F; Budik, S (2003): Expression of different genes during early embryonic development in the horse. 19th Meeting of A.E.T.E., Rostock, 12. - 13. Sept. 2003. 19th Meeting of A.E.T.E., Rostock, 12. - 13. Sept. 2003.

Dunham, S; Klein, D; Bruce, J; Golder, M; Jarrett, O; Neil, J.C (2003): Prime boost vaccination against Feline Immunodeficiency Virus. 15th International Workshop on Retroviral Pathogenesis, Glasgow, UK, 17.-21.09.2003. 15th International Workshop on Retroviral Pathogenesis, Glasgow, UK, 17.-21.09.2003.

Haining, H; Knapp, E; Willett, B; Klein, D; Hosie, M (2003): Studies of the pathogeniciety of primary isolates of Feline Immunodeficency Virus from cats at different stages of disease. 15th International Workshop on Retroviral Pathogenesis, Glasgow, UK, 17.-21.09.2003. 15th International Workshop on Retroviral Pathogenesis, Glasgow, UK, 17.-21.09.2003.

Klein, D; Steinrigl, A (2003): Phylogeny of feline immunodeficiency virus in Central Europe: a prerequisite for vaccination and molecular diagnostics. 6th International Congress of Veterinary Virology, St. Malo, Frankreich, 24.-27.08.2003. 6th International Congress of Veterinary Virology, St. Malo, Frankreich, 24.-27.08.2003.

Leichsenring, B; Knapp, B; Salmons, B; Günzburg, W. H; Klein, D (2003): Influence of cis-acting elements on the transduction efficiency of retroviral vectors. Jahrestagung der Gesellschaft für Virologie 2003 , Berlin, 26.3.-29.3.2003. Jahrestagung der Gesellschaft für Virologie 2003 , Berlin, 26.3.-29.3.2003.

Salmons,B; Hlavaty, J; Leichsenring, B; Stracke, A; Klein, D; Günzburg, W.H; Renner M (2003): Development of high titer, tissue-specific retroviral vector for in vivo tumour therapy. First Workshop of the Study Group in the Society for Virology on viral vectors, Würzburg, Germany, 22.-24.09.2003. First Workshop of the Study Group in the Society for Virology on viral vectors, Würzburg, Germany, 22.-24.09.2003.

Steinrigl, A; Hinterreiter, S; Hohenadl, C; Günzburg, W.H; Klein, D (2003): Development of Improved Models to Study Transduction of Non-dividing Cells by Feline Immunodeficiency Virus. 15 th international workshop on retroviral pathogenesis 2003, Glasgow, UK , 17-21.09.2003. 15 th international workshop on retroviral pathogenesis 2003, Glasgow, UK , 17-21.09.2003.

Steinrigl, A; Klein, D (2003): Phylogeny of feline immunodeficiency virus in Central Europe: a prerequisite for vaccination and molecular diagnostics. Jahrestagung der Gesellschaft für Virologie, Berlin, 26.3. - 29.3.2003. Jahrestagung der Gesellschaft für Virologie, Berlin, 26.3. - 29.3.2003.

Popular Science Article

Aurich, C; Budik, S; Walter, I; Klein, D; Pittner, F (2003): Untersuchungen zur Charakterisierung der frühembryonalen Genexpression beim Pferd. Uni Vet Wien Report (2003), 3 1-2.

2002

Journal Article

** Hosie, MJ; Willett, BJ; Klein, D; Dunsford, TH; Cannon, C; Shimojima, M; Neil, JC; Jarrett, O (2002): Evolution of replication efficiency following infection with a molecularly cloned feline immunodeficiency virus of low virulence. J Virol. 2002; 76(12):6062-6072
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** Klein, D (2002): Strike against cancer. Trends Mol Med. 2002; 8(1):7

** Klein, D (2002): Quantification using real-time PCR technology: applications and limitations. Trends Mol Med. 2002; 8(6):257-260

** Oztürk-Winder, F; Renner, M; Klein, D; Müller, M; Salmons, B; Günzburg, WH (2002): The murine whey acidic protein promoter directs expression to human mammary tumors after retroviral transduction. Cancer Gene Ther (9), 5 421-431.
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Klein, D (2002): New pathway for nuclear entry of HIV-1. Trends in Molecular Medicine (8) 55-55.

Klein, D (2002): How retroviruses ensure expression of their genes. Trends in Molecular Medicine (8) 509-509.

Klein, D (2002): NK cells as a reservoir for HIV-1. Trends in Molecular Medicine (8) 414-414.

Klein, D (2002): Clinical impact of crosstalk during apoptosis induction. Trends in Molecular Medicine (8) 261-262.

Conference Paper in Journal

Klein, D (2002): Gene quantification using multiplex real-time PCR: Advantages and limitations. International Symposium on Predictive Oncology and Intervention Strategies, Paris, France, February 9 - 12, 2002. Cancer Detection and Prevention (26), S1 190-190.

Klein, D; Löhr, M; Karle, P; Renner, M; Saller, R.M; Hain, J; Günzburg, W.H; Salmons, B (2002): Encapsulated cell and retroviral mediated treatment of solid tumors. International Journal of Molecular Medicine 10 ,(Suppl.).

Published (citable) presentations at scientific conferences (A2)

** Klein, D; Günzburg, WH (2002): Modern methodologies for isolation/typing of animal-borne human pathogens: A research update on epidemiological tools - II. Quantitative real-time PCR. 105-114.-Conference on Food Safety in the Pre-Harvest Phase; ,; Vienna, Austria. IN: Smulders, FJM [Hrsg.]: Food safety assurance in the pre-harvest phase;

Callanan, J.J; Ryan, G; Klein, D; Knapp, E; Hosie, M.J; Grimes, T; Mabruk, M.J.E.M.F; Jarrett, O (2002): Feline immunodeficiency viral and proviral load dynamics within the CNS of cats during the acute phase following intravenous infection. 6th International Feline Retrovirus Research Symposium, Amelia Island, Florida, 02 .- 05.12.2002. 6th International Feline Retrovirus Research Symposium, Amelia Island, Florida, 02 .- 05.12.2002.

Haining, H; Willett, B; Knapp, E; Jarrett, O; Neil, J.C; Klein, D; Hosie, M.J (2002): CXCR4 usage and virulence of field isolates from asymptomaitc and terminally ill FIV infected cats. 6th International Feline Retrovirus Research Symposium, Amelia Island, Florida, 2.-5.12.2002. 6th International Feline Retrovirus Research Symposium, Amelia Island, Florida, 2.-5.12.2002.

Hartmann, K; Stengel, C; Klein, D; Egberink, H; Balzarini, J (2002): Efficacy of the chemokine receptor inhibitor 1,1-bis-1,4,8,11-tetraazacyclotetradekan against feline immunodeficiency virus infection. 6th International Feline Retrovirus Research Symposium, Amelia Island, Florida, 2.-5.12.2002. 6th International Feline Retrovirus Research Symposium, Amelia Island, Florida, 2.-5.12.2002.

Hlavaty, J; Stracke, A; Klein, D; Salmons, B; Günzburg, W.H; Renner, M (2002): Improvement of tissue-specific retroviral vectors. Joint Annual Meeting of the ÖGBM, ÖGGGT, ÖGBT & ANGT, Salzburg, 16. ¿ 18.09.2002. Joint Annual Meeting of the ÖGBM, ÖGGGT, ÖGBT & ANGT, Salzburg, 16. ¿ 18.09.2002.

Hlavaty, J; Stracke, A; Klein, D; Salmons, B; Günzburg, W.H; Renner, M (2002): Improvement of tissue-specific retroviral vectors. Jahrestagung der Gesellschaft für Virologie 2002 , Erlangen, 8.-11.04.2002. Jahrestagung der Gesellschaft für Virologie 2002 , Erlangen, 8.-11.04.2002.

Hlavaty, J; Stracke, A; Klein, D; Salmons, B; Günzburg, W.H; Renner, M (2002): High titer expression targeted retroviral vectors. 10th Meeting of the European Society of Gene Therapy, Antibes, France, 13. -16.10.2002. 10th Meeting of the European Society of Gene Therapy, Antibes, France, 13. -16.10.2002.

Klein, D; Günzburg, W.H; Salmons, B (2002): Vector optimisation using real-time PCR assays targeting different steps of the retroviral life cycle. FORGEN-Symposium ¿Genes for Therapy and Prevention of Disease¿, Kloster Banz, 21. ¿ 23.02.2002. FORGEN-Symposium ¿Genes for Therapy and Prevention of Disease¿, Kloster Banz, 21. ¿ 23.02.2002.

Klein, D; Steinrigl, A (2002): Phylogeny of Feline Immunodeficiency Virus: implications for virus quantification and vaccination strategies. -28. Jahrestagung der Österreichischen Gesellschaft für Hygiene, Mikrobiologie und Präventivmedizin; MAY 7-10, 2002; Meran. Abstract 9;

Klein, D; Steinrigl, A (2002): Lentiviral sequence variations: implications for molecular diagnostics and virus quantification. EC Cost Consensus Conference on Small Ruminant Lentiviruses, Lyon, 6. - 7.09.2002. EC Cost Consensus Conference on Small Ruminant Lentiviruses, Lyon, 6. - 7.09.2002.

Klein, D; Steinrigl, A (2002): Phylogeny of Feline Immunodeficiency Virus in Central Europe: Implications for virus quantification and antiviral strategies. 6th International Feline Retrovirus Research Symposium, Amelia Island, Florida, 2.-5.12.2002. 6th International Feline Retrovirus Research Symposium, Amelia Island, Florida, 2.-5.12.2002.

Ridha, S; Willett, B; Hosie, M.J; Klein, D (2002): Reversion to virulence of an apathogenic molecular clone of FIV?. 6th International Feline Retrovirus Research Symposium, Amelia Island, Florida, 2.-5.12.2002. 6th International Feline Retrovirus Research Symposium, Amelia Island, Florida, 2.-5.12.2002.

Ryan, G; Klein, D; Brankin, B; McQuaid, S; Hosie, M; Grimes, T; Mabruk, MJEMF; Callanan, JJ (2002): Measurements of viral loads within the feline brain experimentally infected with feline immunodeficiency virus and assessment of blood brian barrier. XIIth International Congress of Virology of the International Union of Microbiological Societies (IUMS), Paris, 27.07. - 1.08.2002. XIIth International Congress of Virology of the International Union of Microbiological Societies (IUMS), Paris, 27.07. - 1.08.2002.

Schmoll, F; Indik, S; Sipos, W; Klein, D (2002): Phylogenetic analysis of Austrian PRRSV field isolates. International Pig Veterinary Society 2002 Congress, Ames/Iowa, 2. ¿ 5.06.2002 . International Pig Veterinary Society 2002 Congress, Ames/Iowa, 2. ¿ 5.06.2002 .

Sipos, W; Indik, S; Irgang, P; Klein, D; Schuh, M; Schmoll, F (2002): Transmission of EU-ML-PRRSV (ProcilisÒPRRS, Intervet) from vaccinated to non-vaccinated gilts. International Pig Veterinary Society 2002 Congress, Ames/Iowa, 2. -5.06.2002 . International Pig Veterinary Society 2002 Congress, Ames/Iowa, 2. -5.06.2002 .

Steinrigl, A; Klein, D (2002): Phylogenetic analysis of feline immunodeficiency virus as prerequisite for lentiviral vaccination strategies. Jahrestagung der Gesellschaft für Virologie 2002 , Erlangen, 8.-11.04.2002. Jahrestagung der Gesellschaft für Virologie 2002 , Erlangen, 8.-11.04.2002.

2001

Journal Article

** Hein, I; Klein, D; Lehner, A; Bubert, A; Brandl, E; Wagner, M (2001): Detection and quantification of the iap gene of Listeria monocytogenes and Listeria innocua by a new real-time quantitative PCR assay. Res Microbiol. 2001; 152(1):37-46

** Indraccolo, S; Minuzzo, S; Roccaforte, F; Zamarchi, R; Habeler, W; Stievano, L; Tosello, V; Klein, D; Günzburg, WH; Basso, G; Chieco-Bianchi, L; Amadori, A (2001): Effects of CD2 locus control region sequences on gene expression by retroviral and lentiviral vectors. Blood. 2001; 98(13):3607-3617
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** Klein, D (2001): Benefits from early treatment of HIV-1 infection. Trends Mol Med. 2001; 7(1):9

** Klein, D; Leutenegger, CM; Bahula, C; Gold, P; Hofmann-Lehmann, R; Salmons, B; Lutz, H; Gunzburg, WH (2001): Influence of preassay and sequence variations on viral load determination by a multiplex real-time reverse transcriptase-polymerase chain reaction for feline immunodeficiency virus. J Acquir Immune Defic Syndr. 2001; 26(1):8-20

** Tabotta, W; Klein, D; Hohenadl, C; Salmons, B; Günzburg, WH (2001): Genetic reshuffling reconstitutes functional expression cassettes in retroviral vectors. J Gene Med. 2001; 3(5):418-426

Klein, D (2001): Practically perfect (Book review on 'A Practical approach: RNA viruses'). Trends in Molecular Medicine (7), 4 186-186.

Klein, D (2001): HIV latency in naïve T cells. Trends in Molecular Medicine(Commentary) (7), 7 285-285.

Klein, D (2001): Filoviruses: new cofactor for cell entry. Trends in Molecular Medicine(Commentary) (7), 10 433-433.

Conference Paper in Journal

** Ryan, G; Klein, D; Callanan, S; Hosie, M; Grimes, T; Kay, E; Leader, M; Mabruk, MJEMF (2001): Viral loads in the feline brain infected experimentally with feline immunodeficiency virus: A model for HIV-1 neuropathogenesis. 183rd Meeting of Pathological Society of Great Britain & Ireland, Liverpool, JUL 03-06, 2001. J PATHOL (195), Supplement 9A-9A.

Ryan, G; Klein, D; Callanan, S; Hosie, M; Mabruk, MJEMF; Grimes, T (2001): Viral loads in the feline brain infected with feline immunodeficiency virus: A model for HIV-1 neuropathogenesis. Irish Journal of Medical Science (170), Suppl. 2 76-76.

Published (citable) presentations at scientific conferences (A2)

Charlton, MA; Anderton, TL; May, JP; Klein, D; Preston, A; Slater, JD; Maskell, DJ (2001): A novel equine respiratory air-interface organ culture system. 52-52.-World Equine Airways Symposium; July 2001; Edinburgh.

Hosie, MJ; Willett, B; Dunsford, TH; Klein, D; Binley, JM; Hoxie, JA (2001): Partial protection against virulent isolate of feline immunodeficiency virus following immunisation with a whole inactivated virus vaccine. 69-69.-6th European Conference on Experimental AIDS Research; 23.-26.06.2001 ; Edinburgh.

Klein, D (2001): Influence of molecular epidemiology on diagnosis and quantification using real-time PCR assays. 285-287.-Xth International Symposium of Veterinary Laboratory Diagnosticians; JUL 4-7, 2001 ; Salsomaggiore,Italy.

Klein, D (2001): Influence of molecular epidemiology on viral load estimation using real-time PCR assays. 61-61.-6th European Conference on Experimental AIDS Research; JUN 23-26, 2001 ; Edinburgh.

Klein, D; Fröschl, N; Knapp, E; Salmons, B; Günzburg, WH (2001): Influence of deletions in the c-terminal region of Mo-MLV integrase on the kinetics of the retroviral life cycle. 10-10.-2nd International Conference on Retroviral Integrase; OCT 28-30, 2001; Paris, FR.

Klein, D; Fröschl, N; Knapp, E; Salmons, B; Günzburg, WH (2001): Influence of mutations in the integrase of Mo-MLV on the kinetics of the retroviral life cycle. 286-286.-CSHL Retroviruses Meeting; MAY 22-27, 2001; Cold Spring Harbor, USA.

Klein, D; Knapp, E; Fröschl, N; Salmons, B; Günzburg, WH (2001): Gene transfer estimation and optimisation: Interplay between FACS analysis and multiplex real-time PCR. 35-35.-XV. Arbeitstagung: Anwendung der Durchflußzytometrie in der klinischen Zelldiagnostik; APR 4-6, 2001; Regensburg.

Leichsenring, B; Knapp, E; Salmons, B; Günzburg, W.H; Klein, D (2001): Inverted repeat of Mo-MLV based vectors is not sufficient as the integrase attachment site. 2nd International Conference on Retroviral Integrase, Paris, FR, 28. - 30.10.2001. 2nd International Conference on Retroviral Integrase, Paris, FR, 28. - 30.10.2001.

Ryan, G; Klein, D; Hosie, M; Mabruk, MJEMF; Grimes, T; Callanan, S (2001): Viral loads in the feline brain infected with feline immunodeficiency virus: A model for HIV-1 neuropathogenesis. Conway Institute Research Seminar, Conway, IR, Sept. 2001 . Conway Institute Research Seminar, Conway, IR, Sept. 2001 .

2000

Journal Article

** Hosie, MJ; Dunsford, T; Klein, D; Willett, BJ; Cannon, C; Osborne, R; Macdonald, J; Spibey, N; Mackay, N; Jarrett, O; Neil, JC (2000): Vaccination with inactivated virus but not viral DNA reduces virus load following challenge with a heterologous and virulent isolate of feline immunodeficiency virus. J Virol. 2000; 74(20):9403-9411
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** Indik, S; Valícek, L; Klein, D; Klánová, J (2000): Variations in the major envelope glycoprotein GP5 of Czech strains of porcine reproductive and respiratory syndrome virus. J Gen Virol (81), Pt 10 2497-2502.
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** Klein, D (2000): Clinical gene therapy back in the race. Mol Med Today. 2000; 6(9):338

** Klein, D; Bugl, B; Günzburg, WH; Salmons, B (2000): Accurate estimation of transduction efficiency necessitates a multiplex real-time PCR. Gene Ther. 2000; 7(6):458-463
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** Klein, D; Musil, C; Hirt, R; Gold, P; Thalhammer, JG; Günzburg, WH (2000): Möglichkeiten und Grenzen neuer molekularer Untersuchungsmethoden in der klinischen Mikrobiologie: Dargestellt am Beispiel des Felinen Immundefizienzvirus. Wiener Tierärztliche Monatsschrift (87) 269-277.

** Uckert, W; Becker, C; Gladow, M; Klein, D; Kammertoens, T; Pedersen, L; Blankenstein, T (2000): Efficient gene transfer into primary human CD8+ T lymphocytes by MuLV-10A1 retrovirus pseudotype. Hum Gene Ther. 2000; 11(7):1005-1014

** Wagner, M; Lehner, A; Klein, D; Buber, A (2000): Single-strand conformation polymorphisms in the hly gene and polymerase chain reaction analysis of a repeat region in the iap gene to identify and type Listeria monocytogenes. J Food Prot. 2000; 63(3):332-336

** Walter, I; Fleischmann, M; Klein, D; Müller, M; Salmons, B; Günzburg, WH; Renner, M; Gelbmann, W (2000): Rapid and sensitive detection of enhanced green fluorescent protein expression in paraffin sections by confocal laser scanning microscopy. Histochem J. 2000; 32(2):99-103

Conference Paper in Journal

Klein, D (2000): Gene transfer estimation by multiplex real-time PCR: Advantages and limitations. First International Symposium on Gene Technology and Skin Gene Therapy, Essen, APR 14-15, 2000. Archives of Dermatological Research (292), 4 209-209.

Walter, I; Petznek, H; Klein, D; Rigler, D; Müller, M; Salmons, B; Günzburg, W.H; Renner, M. Scanning (2000): Confocal laser scanning microscopy of enhanced green fluorescent protein (EGFP) expression in transgenic mice. San Antonio, Texas, 9.-12.05.2000. Scanning 22,

Published (citable) presentations at scientific conferences (A2)

Cuisinier, A.M; Verdier, A.S; Klein, D; Aubert, A (2000): Suppression following DNA immunizations and challenge with the feline immunideficiency virus (FIV). Fifth International Feline Retrovirus Research Symposium , Cannes, F, 2000. Fifth International Feline Retrovirus Research Symposium , Cannes, F, 2000.

Froeschl, N; Knapp, E; Salmons, B; Günzburg, W.H; Klein, D (2000): Kinetics of the retroviral life cycle investigated by multiplex real-time PCR assays. International Conference and Workshop on Animal Retroviruses, UK, 2000. International Conference and Workshop on Animal Retroviruses, UK, 2000.

Hosie, M.J; Dunsford, T.H; Klein, D; Willett, B.J; MacDonald, J; Spibey, N; Jarrett, O; Neil, J.C (2000): Viral factors in the pathogenesis of FIV infection. Fifth International Feline Retrovirus Research Symposium, Cannes, FR, 2000. Fifth International Feline Retrovirus Research Symposium, Cannes, FR, 2000.

Indik, S; Valicek, L; Klein, D; Klanova, J (2000): Comparison of the ORF5 sequences of Czech PRRSV isolates. 5th International Congress of the European Society for Veterinary Virology, Brescia, IT , 2000. 5th International Congress of the European Society for Veterinary Virology, Brescia, IT , 2000.

Klein, D; Bugl, B; Günzburg, W.H; Salmons, B (2000): Gene transfer estimation: comparison of FACS and real-time PCR. XIV Arbeitstagung, Anwendungen der Durchflußzytometrie in der klinischen Zelldiagnostik¿, Regensburg, DE, April 2000. XIV Arbeitstagung, Anwendungen der Durchflußzytometrie in der klinischen Zelldiagnostik¿, Regensburg, DE, April 2000.

Klein, D; Günzburg, W. H; Salmons, B (2000): Influence of mutations in the integrase on the kinetics of the retroviral life cycle. Jahrestagung der Gesellschaft für Virologie, Wien, 2000. Jahrestagung der Gesellschaft für Virologie, Wien, 2000.

Klein, D; Günzburg, W.H (2000): Modern methodologies for isolation/typing of animal-borne human pathogens: a research update on epidemiological tools; Part II: Quantitative Real-time PCR. Euroconference -Food Safety Assurance in the Pre-Harvest Phase, Wien, 2000. Euroconference -Food Safety Assurance in the Pre-Harvest Phase, Wien, 2000.

Klein, D; Günzburg, W.H; Salmons, B (2000): Gene transfer estimation by multiplex real-time PCR: Advantages and limitations. Forgen-Symposium -Genes for Therapy and Prevention of Disease, Wildbad Kreuth, 2000. Forgen-Symposium -Genes for Therapy and Prevention of Disease, Wildbad Kreuth, 2000.

Klein, D; Rungaldier, S; Krassnig, R (2000): Nachweis und Quantifizierung von Maedi/Visna-Virus mittels Real-time PCR. AVID-Mitteilungen, DE, 2000. AVID-Mitteilungen, DE, 2000.

Klein, D; Salmons, B; Günzburg, W. H (2000): Influence of pre-assay and sequence variations on lentiviral load determination by a multiplex real-time PCR. Jahrestagung der Gesellschaft für Virologie, Wien, 2000. Jahrestagung der Gesellschaft für Virologie, Wien, 2000.

Klein, D; Salmons, B; Günzburg, W.H (2000): Viral load determination using Real-time PCR: Detailed study on factors influencing precision and accuracy. 5th International Feline Retrovirus Research Symposium, Cannes, FR, 2000. 5th International Feline Retrovirus Research Symposium, Cannes, FR, 2000.

Klein, D; Stengel, C; Musil, C; Hirt, R; Schlötterer, C; Hartmann, K; Thalhammer, J.G; Günzburg, W.H (2000): Molecular epidemiology of feline immunodeficiency virus: Implications for virus quantification and vaccination startegies. DVG-Fachgruppe, Wien, 2000. DVG-Fachgruppe, Wien, 2000.

Loeschenberger, K; Klein, D; Peschke, R; Voracek, T; Zenker, W; Edelhofer, R; Prosl, H (2000): Hohe Mortalitätsrate bei Felis-manul-Welpen durch Toxoplasma gondii. XXXIV. Tagung der Österreichischen Gesellschaft für Tropenmedizin und Parasitologie, Wien, 17.11.2000. XXXIV. Tagung der Österreichischen Gesellschaft für Tropenmedizin und Parasitologie, Wien, 17.11.2000.

Löschenberger, K; Klein, D; Peschke, R; Voracek, T; Zenker, W; Edelhofer, R; Prosl, H (2000): Hohe Mortalitätsrate bei Felis manul-Welpen durch Toxoplasma gondii. 11-11.-XXXIV. Tagung d. Österreichische Gesellschaft für Parasitologie und Tropenmedizin; 2000; Wien.

Rungaldier, S; Froeschl, N; Knapp, E; Krassnig, R; Klein, D (2000): Comparison of clinical signs and proviral load by Maedi/Visna specific real-time PCR. International Conference and Workshop on Animal Retroviruses, Queen's College, Cambridge, 2002. International Conference and Workshop on Animal Retroviruses, Queen's College, Cambridge, 2002.

Postdoctoral lecture qualification

Klein, D (2000): Quantitative Molekularvirologie: Von der Diagnose zur Gentherapie. Habilitationsschrift, Vet. Med. Univ. Wien, pp. 150.

1999

Journal Article

** Klein, D; Janda, P; Steinborn, R; Müller, M; Salmons, B; Günzburg, WH (1999): Proviral load determination of different feline immunodeficiency virus isolates using real-time polymerase chain reaction: influence of mismatches on quantification. Electrophoresis. 1999; 20(2):291-299

** Leutenegger, CM; Klein, D; Hofmann-Lehmann, R; Mislin, C; Hummel, U; Böni, J; Boretti, F; Guenzburg, WH; Lutz, H (1999): Rapid feline immunodeficiency virus provirus quantitation by polymerase chain reaction using the TaqMan fluorogenic real-time detection system. J Virol Methods. 1999; 78(1-2):105-116

Conference Paper in Journal

Gelbmann, W; Klein, D; Walter, I; Müller, M; Fleischmann, M; Hanak-Hammerl, A; Renner, M; Salmons, B; Günzburg, W.H (1999): In Vivo Gene Therapy Models Targeting Metastatic Breast Cancer. 7th Meeting of the European Society of Gene Therapy, München, DE, 1999. The Journal of Gene Medicine 1,(Suppl. 6).

Klein, D (1999): Molecular Epidemiology of FIV: Implications for Virus Quantification and Vaccination Stratgies. The Infectious Disease Review 1,(4).

Klein, D; Bugl, B; Günzburg, W.H; Salmons, B (1999): Accurate determination of transduction efficiency by multiplex Real-Time PCR. Seventh Meeting of the European Society of Gene Therapy, Munich, DE. The Journal of Gene Medicine 1,(Suppl. 6).

Klein, D; Janda, P; Hartmann, K; Salmons, B; Günzburg, WH (1999): Influence of mismatches on viral load determination by real-time PCR. 9th European Congress of Clinical Microbiology and Infectious Diseases, Berlin , MAR 21–24, 1999. Clinical Microbiology Infection (5), Suppl.3 209-210.

Published (citable) presentations at scientific conferences (A2)

Hein, I; Lehner, A; Klein, D; Wagner, M; Brandl, E (1999): Detection and enumeration of Listeria monocytogenes and Listeria innocua from dairy products by means of TaqMan real-Time PCR. 9th European Congress of Clinical Microbiology and Infectious Diseases, Berlin, Deutschland, 21.-24. März 1999. 9th European Congress of Clinical Microbiology and Infectious Diseases, Berlin, Deutschland, 21.-24. März 1999.

Hein, I; Lehner, A; Wagner, M; Klein, D; Brandl, E (1999): Nachweis, Differenzierung und Quantifizierung von Listeria monocytogenes und Listeria innocua in Milch mittels Real-Time Quantitative PCR (TaqMan¿). 40. Arbeitstagung des Arbeitsgebietes , Garmisch-Partenkirchen, Deutschland, 29.9.-1.10.1999. 40. Arbeitstagung des Arbeitsgebietes , Garmisch-Partenkirchen, Deutschland, 29.9.-1.10.1999.

Klein, D (1999): Molecular Epidemiology of FIV: Implications for Virus Quantification and Vaccination Stratgies. 5th European Workshop on Virus Evolution and Molecular Epidemiology, Leuven, NL, 1999. 5th European Workshop on Virus Evolution and Molecular Epidemiology, Leuven, NL, 1999.

Klein, D (1999): Viral Load Determination using Real-time PCR: Advantages and Limitations. Satellite Workshop on Lentiviruses of Sheep and Goats: Pathogenesis, Diagnosis and Prevention, Arcachon, FR, 1999. Satellite Workshop on Lentiviruses of Sheep and Goats: Pathogenesis, Diagnosis and Prevention, Arcachon, FR, 1999.

Klein, D; Janda, P; Günzburg, W.H; Hartmann, K (1999): FIV: Molekular-epidemiologische Untersuchungen. 8. Jahrestagung der Fachgruppe -Innere Medizin und klinische Laboratoriumsdiagnostik¿, München, DE, 1999. 8. Jahrestagung der Fachgruppe -Innere Medizin und klinische Laboratoriumsdiagnostik¿, München, DE, 1999.

1998

Journal Article

** Günzburg, WH; Karle, P; Mrochen, S; Sparmann, G; Saller, R; Klein, D; Uckert, W; Salmons, B (1998): Regulated gene expression after retroviral vector-mediated delivery of cancer-relevant therapeutic genes. Recent Results Cancer Res. 1998; 144:11-26

** Mrochen, S; Klein, D; Nikol, S; Smith, JR; Salmons, B; Günzburg, WH (1998): Inducible expression of p21WAF-1/CIP-1/SDI-1 from a promoter conversion retroviral vector. J Mol Med (75), 11-12 820-828.

** Steinborn, R; Zakhartchenko, V; Jelyazkov, J; Klein, D; Wolf, E; Müller, M; Brem, G (1998): Composition of parental mitochondrial DNA in cloned bovine embryos. FEBS Lett. 1998; 426(3):352-356

Published (citable) presentations at scientific conferences (A2)

Klein, D (1998): Real-time PCR: Eine neue Methode zur Virusquantifizierung. 17. Arbeits- und Fortbildungstagung des AVID, Kloster Banz, 23.-25.09.98. 17. Arbeits- und Fortbildungstagung des AVID, Kloster Banz, 23.-25.09.98.

Klein, D; Leutenegger, C, Salmons, B; Lutz, H; Gunzburg, W.H (1998): Real-time PCR: A powerful tool to quantify -viral loads- of FIV. International Symposium on Strategies in Virus-Host Relationships, Lyon, 16.-18-02.1998. International Symposium on Strategies in Virus-Host Relationships, Lyon, 16.-18-02.1998.

Klein, D; Leutenegger, C; Salmons, B; Lutz, H; Günzburg, W.H (1998): Real-time PCR: A powerful tool to quantify -viral loads. Fourth International Feline Retrovirus Research Symposium, Glasgow, 28.-30.05.1998. Fourth International Feline Retrovirus Research Symposium, Glasgow, 28.-30.05.1998.

Klein, D; Sölkner, B; Salmons, B; Günzburg, W.H (1998): Comparison of transduction efficiencies estimated by FACS and Real-time PCR. FORGEN Symposium: Genes for Therapy and Prevention of Diseases, Kloster Banz, 27. - 29.09.98. FORGEN Symposium: Genes for Therapy and Prevention of Diseases, Kloster Banz, 27. - 29.09.98.

Leutenegger, C.M; Klein, D; Hofmann-Lehmann, R; Mislin, U; Hummel, U; Böni, J; Boretti, F; Guenzburg, W.H; Lutz, H (1998): Taqman method for Quantification of FIV DNA load and feline cytokine gene expression. Fourth International Feline Retrovirus Research Symposium, Glasgow, 28.-30.05.1998. Fourth International Feline Retrovirus Research Symposium, Glasgow, 28.-30.05.1998.

Wagner, M; Lehner, A; Klein, D; Brandl, E; Bubert, A (1998): Results on differentiation of listeria monocytogenes by capillary electrophoresis of PCR-products derived from a repeat region in the iap gene. 13th International Symposium on Problems of Listeriosis, Halifax, Kanada, 28.06.-03.07.1998. 13th International Symposium on Problems of Listeriosis, Halifax, Kanada, 28.06.-03.07.1998.

Original Article in Series

Salmons, B; Saller, RM; Mrochen, S; Öztürk, F; Klein, D; Günzburg, WH (1998): Inducible gene expression from promoter conversion retroviral vectors. IN: Ahmad, F [Hrsg.]: Advances in Gene Technology: Molecular Biology in the Conquest of the disease. Oxford, Oxford University Press, pp. 179-180.

1997

Journal Article

** Klein, D; Indraccolo, S; von Rombs, K; Amadori, A; Salmons, B; Günzburg, WH (1997): Rapid identification of viable retrovirus-transduced cells using the green fluorescent protein as a marker. Gene Ther. 1997; 4(11):125-160
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Original Article in Series

Günzburg, WH; Klein, D; Mrochen S; Saller, R; Öztürk-Winder; Karle, P; Müller, P; Löhr, M; Salmons, B (1997): Expression targeting of therapeutic genes carried by retroviral vectors to breast and other cancers. IN: Boiron, M [Hrsg.]: Eurocancer 97. Paris, France, John Libbey Eurotext Press, pp. 321-322.

Popular Science Article

Klein, D; Salmons, B; Günzburg, WH (1997): Comparison of viral vectors: Implications for gene therapy. BIOforum International (1) 104-109.

1996

Journal Article

** Müller-Werdan, U; Koidl, B; Autenrieth, A; Klein, D; Werdan, K; Hammer, C (1996): Xenoreactive natural antibodies and induced antibodies--their effects of beating cardiomyocytes as a model of a xenograft. Mol Cell Biochem. 1996; 160-161(8):315-323

Conference Paper in Journal

Müller-Werdan, U; Koidl, B; Autenrieth, A; Klein, D; Werdan, K; Hammer, C (1996): Unterschiedliche Mechanismen der hyperakuten Abstoßungsreaktion bei Xenotransplantation (XT) durch präformierte natürliche Antikörper (PNAk) und induzierte Antikörper (IA). Zeitschrift für Kardiologie 85,(Suppl. 2).

Published (citable) presentations at scientific conferences (A2)

Klein, D; von Rombs, K; Salmons, B; Günzburg, W.H (1996): Comparison of Marker Genes (GFP and ß-Gal) in the Context of a Retroviral Vector. 8th Workshop on the Pathogenesis of Animal Retroviruses, St. Malo, 23.-25.10.1996. 8th Workshop on the Pathogenesis of Animal Retroviruses, St. Malo, 23.-25.10.1996.

1995

Journal Article

** Müller-Werdan, U; Koidl, B; Autenrieth, A; Klein, D; Zander, M; Werdan, K; Hammer, C (1995): Preformed natural antibodies: their impact on contractile activity of cardiomyocytes. Transplant Proc. 1995; 27(3):2109-2111

Conference Paper in Journal

Müller-Werdan, U; Koidl, B; Autenrieth, A; Klein, D; Werdan, K; Hammer, C (1995): Cardiodepressent effects of xenoreactive natural antibodies in cardiomyocytes. Shock 3,(Suppl. 1).

Müller-Werdan, U; Koidl, B; Autenrieth, A; Klein, D; Werdan, K; Hammer, C (1995): Xenoreactive natural antibodies (XNA) - cardiodepressent effects in cardiomyocytes of neonatal rats (RCM). Journal of Molecular and Cellular Cardiology 27,

1994

Journal Article

** Müller, U; Klein, D; Zander, M; Werdan, K; Hammer, C (1994): Preformed natural antibodies: their impact on beating neonatal rat cardiomyocytes. Transplant Proc. 1994; 26(2):1000-1002

** Müller-Werdan, U; Klein, D; Zander, M; Werdan, K; Hammer, C (1994): Beating neonatal rat cardiomyocytes as a model to study the role of xenoreactive natural antibodies in xenotransplantation. Transplantation. 1994; 58(12):1403-1409

Conference Paper in Journal

Müller, U; Klein, D; Zander, M; Werdan, K; Hammer, C (1994): Die Wirkung präformierter natürlicher Antikörper (PNAk) auf das Schlagverhalten von Rattenkardiomyozyten. Zeitschrift für Kardiologie 83,(Suppl. 1).

Müller, U; Klein, D; Zander, M; Werdan, K; Hammer, C (1994): Temporal Impairment of Cardiomyocytes- contractility by xenoreactive natural antibodies. The Canadian Journal of Cardiology 10,(Suppl. A).

Original Article in Series

Klein, D; Müller, U; Zander, M; Werdan, K; Hammer, C (1994): Die Wirkung von Präformierten Natürlichen Antikörpern (PNAK) auf schlagende Herzmuskelzellkulturen von neonatalen Ratten. IN: Chirurgisches Forum 1994 für experimentelle und klinische Forschung. Berlin, Heidelberg, Springer, pp. 157-162.

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