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Virus-host interactions

Abstract
Retroviruses are among the most extensively studied virus families. Since most research approaches have
focused mainly on mechanisms of the retroviral life cycle itself, profound knowledge of the details of the
interactions between retroviruses and their hosts is very limited. However, the decoding of the human genome
has provided the basis to close the gap between molecular virology and cellular biology. This rapidly expanding
area should provide a deeper understanding of the pathways and components used by different retroviruses in
more detail. This knowledge in turn will enable the development of better antiviral strategies and improve their
usage as vectors for gene therapy.


Project leader
Klein Dieter
Duration
01.01.1999-31.12.2007
Type of Research
Basic research
Vetmed Research Units
Institute of Virology
Funded by
91 Publications

Klein, D (2012): Feline Immunodeficiency Virus as a natural animal model for HIV vaccine research: Advantages and limitations. 57-57.-BIT’s 4th Annual World Vaccine Congress; MAR 26-28, 2012; Beijing, CHINA.

Ryan, G; Klein, D; Callanan, S; Hosie, M; Grimes, T; Kay, E; Leader, M; Mabruk, MJEMF (2001): Viral loads in the feline brain infected experimentally with feline immunodeficiency virus: A model for HIV-1 neuropathogenesis. 183rd Meeting of Pathological Society of Great Britain & Ireland, Liverpool, JUL 03-06, 2001. J PATHOL (195), Supplement 9A-9A.

Klein, D; Günzburg, WH (2002): Modern methodologies for isolation/typing of animal-borne human pathogens: A research update on epidemiological tools - II. Quantitative real-time PCR. 105-114.-Conference on Food Safety in the Pre-Harvest Phase; ,; Vienna, Austria. IN: Smulders, FJM [Hrsg.]: Food safety assurance in the pre-harvest phase;

Jais, A; Klein, D; Wolfesberger, B; Walter, I (2011): Gene expression profile of vascular endothelial growth factor (VEGF) and its receptors in various cell types of the canine lymph node using laser capture microdissection (LCM). Vet Immunol Immunopathol. 2011; 140(3-4):207-214

Klein, D; Ertl, R; Birzele, F; Hildebrandt, T (2011): Feline Immunodeficiency Virus as a Natural Animal Model for HIV Research: New Insights into Virus Host Interactions using Second Generation Sequencing Technology. 254--BIT’s 3rd Annual World Vaccine Congress; MAR 23-25, 2011; Beijing, CHINA.

Ernst, Christina (2010): Identification und Characterization of a cystein-rich Motif in Reverse Transcriptase encoded by the Human L1 element. Bakkalaureatsarbeit, Vet. Med. Univ. Wien, pp. 33.
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Bachler, Barbara (2010): Isolating HIV-1 Envelope Mimotopes Using Polyclonal Sera from Pig-Tailed Macaques Infected with a CCR5-Tropic Subtype-C Simian-Human Immunodeficiency Virus. Master Thesis, Vet. Med. Univ. Wien, pp. 102.

Achleitner, Andrea (2010): Interaction of Programmed Death (PD-1) molecules with T-regulatory cells. Master Thesis, Vet. Med. Univ. Wien, pp. 42.
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Ertl, R; Birzele, F; Hildebrandt, T; Klein, D (2010): Analysis of virus-host interactions in Feline immunodeficiency virus infected cells using massive parallel sequencing technology. 38-38.-22nd International Workshop on Retroviral Pathogenesis; NOV 17-21, 2010; Newport Beach, USA.

Klein, D (2010): Feline Immunschwächeviren: Machen sie Katzen überhaupt krank?. 9-11.-AfT-Herbstsymposium – Virale Infektionskrankheiten bei der Katze; NOV 6, 2010; Munich, GERMANY.

Ertl, R; Birzele, F; Hildebrandt, T; Klein, D (2010): Analysis of virus-host interactions in FIV infected cells using massive parallel sequencing technology. -Illumina User Meeting ; JUL 13-15, 2010; Barcelona.

Ertl, R; Birzele, F; Hildebrandt, T; Klein, D (2010): Analysis of virus-host interactions in FIV infected cells using massive parallel sequencing technology. 56--10th International Feline Retrovirus Research Symposium ; May 23-26, 2010; Charleston, USA.

Ertl, R; Birzele, F; Hildebrandt, T; Klein, D (2010): Analysis of virus-host interactions in FIV infected cells using massive parallel sequencing technology. 48--32. Jahrestagung der ÖGHMP ; MAY 17-20, 2010; Wien.

Sand, C; Stengel, C; Klein, D; Sauter-Louis, C; Hartmann, K (2009): Association of provirus and virus load with the health status of cats naturally infected with feline immunodeficiency virus. 225-225.-19th Annual Congress of the European College of Veterinary Internal Medicine – Companion Animals (ECVIM-CA); SEP 8-10, 2009; Porto, PORTUGAL.

Hofer, M (2008): Establishment and validation of real-time PCR assays for the quantification of different DNA-forms of Feline Immunideficiency Virus. Master Thesis, Vet. Med. Univ. Wien, pp. 120.
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Steinrigl, A; Nosek, D; Ertl, R; Salmons, B; Günzburg, WH; Klein, D (2007): Moloney Murine leukemia virus integrase mutants suggest differences to lentiviruses in the role of extrachromosomal DNA for the viral life cycle. 53--19th International Workshop on Retroviral Pathogenesis; SEP 25-29, 2007; Vienna, AUSTRIA.

Schild, M; Salmons, B; Günzburg, WH; Klein, D; Steinrigl, A (2007): Significance of the central polypurine tract for feline immunodeficiency virus transduction efficiency. 52--19th International Workshop on Retroviral Pathogenesis; SEP 25-29, 2007; Vienna, AUSTRIA.

Steinrigl, A; Ertl, R; Hofer, M; Klein, D (2008): Expression of extrachromosomal DNA is inefficient in Murine Leukemia Virus. 65--9th International Feline Retrovirus Research Symposium; AUG 24-27, 2008; Vienna, AUSTRIA.

Steinrigl, A; Ertl, R; Knapp, E; Langbein, I; Klein, D (2008): Phylogenetic analysis of European FIV-sequences suggests independent introduction of subtypes A and B to Central Europe. 17--9th International Feline Retrovirus Research Symposium; AUG 24-27, 2008; Vienna, AUSTRIA.

Kraase, M; Sloan, R; Klein, D; Willett, BJ; Hosie, MJ (2008): Feline Immunodeficiency Virus env gene quasispecies evolution in experimentally infected cats. 63--9th International Feline Retrovirus Research Symposium; AUG 24-27, 2008; Vienna, AUSTRIA.

Hofer, M; Klein, D; Steinrigl, A (2008): Establishment and validation of real-time PCR assays for the quantification of different forms of Feline Immunodeficiency Virus DNA. 71--9th International Feline Retrovirus Research Symposium; AUG 24-27, 2008; Vienna, AUSTRIA.

Steinrigl, A; Ertl, R; Gansterer, M; Günzburg, WH; Salmons, B; Klein, D (2007): Influence of producer cell DNA carryover on retroviral titer and transduction efficiency estimation. 15th Annual Congress of the European Society of Gene and Cell Therapy, Rotterdam, NETHERLANDS, Netherlands, OCT 27-30, 2007. Hum Gene Ther (18), 10 1036.

Steinrigl, A; Ertl, R; Salmons, B; Günzburg, WH; Klein, D (2007): Comparison between HIV-1 and MoMLV integrase mutants: differences regarding the abundance of 2-LTR circles and expression from extrachromosomal DNA. 176-176.-3rd European Congress of Virology; 01.-05.09.2007; Nürnberg.

Steinrigl, A; Ertl, R; Salmons, B; Günzburg, WH; Klein, D (2007): Estimation of retroviral titre and transduction efficiency by real-time PCR is easily biased by DNA-carryover from transient producer cells. 169-169.-3rd European Congress of Virology; 01.-05.09.2007; Nürnberg.

Steinrigl, A; Nosek, D; Ertl, R; Günzburg, WH; Salmons, B; Klein, D (2007): Mutations in the catalytic core or the C-terminus of murine leukemia virus (MLV) integrase disrupt virion infectivity and exert diverse effects on reverse transcription. Virology. 2007; 362(1):50-59
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Dunham, SP; Bruce, J; Klein, D; Flynn, JN; Golder, MC; MacDonald, S; Jarrett, O; Neil, JC (2006): Prime-boost vaccination using DNA and whole inactivated virus vaccines provides limited protection against virulent feline immunodeficiency virus. Vaccine. 2006; 24(49-50):7095-7108

Steinrigl, A; Quintino, L; Pusch, M; Ertl, R; Klein, D (2006): Factors involved in infection of non-dividing cells by feline immunodeficiency virus. 8th International Feline Retrovirus Research Symposium, Washington, 8.-11.10.2006. 8th International Feline Retrovirus Research Symposium, Washington, 8.-11.10.2006.

Steinrigl, A; Quintino, L; Pusch, M; Ertl, R; Klein, D (2006): Factors involved in infection of non-dividing cells by feline immunodeficiency virus. 7th International Congress of Veterinary Virology, Lissabon, 24.-27.09.2006. 7th International Congress of Veterinary Virology, Lissabon, 24.-27.09.2006.

Klein, D; Pusch, M; Steinrigl, A; Knapp, E; Müller, E; Hübner, J; Langbein, I (2005): Felines Immunschwächevirus: Molekular-epidemiologische Untersuchungen und deren Relevanz für molekulare Diagnostik und Therapiestudien. 51. Jahrestagung der Deutschen Gesellschaft für Kleintiermedizin, Berlin, Germany, 17.-20.11.2005. 51. Jahrestagung der Deutschen Gesellschaft für Kleintiermedizin, Berlin, 17.-20.11.2005.

Steinrigl, A; Quitino, L; Pusch, M; Knapp, E; Klein, D (2005): Factors involved in infection of non-dividing cells by feline immunodeficiency virus. Cold Spring Harbor Laboratory Retrovirus Symposium 2005, Cold Spring Harbor, 24.-29.05.2005. Cold Spring Harbor Laboratory Retrovirus Symposium 2005, Cold Spring Harbor, 24.-29.05.2005.

de Andrés, D; Klein, D; Watt, NJ; Berriatua, E; Torsteinsdottir, S; Blacklaws, BA; Harkiss, GD (2005): Diagnostic tests for small ruminant lentiviruses. Vet Microbiol. 2005; 107(1-2):49-62

Klein, D; Quitino, L; Pusch, M; Steinrigl, A (2005): Factors involved in infection of non-dividing cells by feline immunodeficiency virus. Jahrestagung der Gesellschaft für Virologie 2005, Hannover, 16.-19.03.2005. Jahrestagung der Gesellschaft für Virologie 2005, Hannover, 16.-19.03.2005.

Hosie, MJ; Klein, D; Binley, JM; Dunsford, TH; Jarrett, O; Neil, JC; Knapp, E; Giannecchini, S; Matteucci, D; Bendinelli, M; Hoxie, JA; Willett, BJ (2005): Vaccination with an inactivated virulent feline immunodeficiency virus engineered to express high levels of Env. J Virol. 2005; 79(3):1954-1957
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Klein, D (1998): Viral load quantification with Real-time PCR. EC TaqMan Workshop, Glasgow, 12.02.1998. EC TaqMan Workshop, Glasgow, 12.02.1998.

Klein, D (1999): Phylogenetic analysis of FIV sequences from Austria and Germany. FAVEUR Workshop on Phylogenetic Evolution of FIV, Pisa, 21.05.1999. FAVEUR Workshop on Phylogenetic Evolution of FIV, Pisa, 21.05.1999.

Klein, D (1999): Molekulare Epidemiologie des Felinen Immundefizienzvirus (FIV): Auswirkungen auf Diagnose und Vakzinierung. AG Epidemiologie der VUW Wien, Wien, Austria, 3.12.1999. AG Epidemiologie der VUW Wien, Wien, 3.12.1999.

Klein, D; Leutenegger, C, Salmons, B; Lutz, H; Gunzburg, W.H (1998): Real-time PCR: A powerful tool to quantify -viral loads- of FIV. International Symposium on Strategies in Virus-Host Relationships, Lyon, 16.-18-02.1998. International Symposium on Strategies in Virus-Host Relationships, Lyon, 16.-18-02.1998.

Leutenegger, C.M; Klein, D; Hofmann-Lehmann, R; Mislin, U; Hummel, U; Böni, J; Boretti, F; Guenzburg, W.H; Lutz, H (1998): Taqman method for Quantification of FIV DNA load and feline cytokine gene expression. Fourth International Feline Retrovirus Research Symposium, Glasgow, 28.-30.05.1998. Fourth International Feline Retrovirus Research Symposium, Glasgow, 28.-30.05.1998.

Klein, D; Leutenegger, C; Salmons, B; Lutz, H; Günzburg, W.H (1998): Real-time PCR: A powerful tool to quantify -viral loads. Fourth International Feline Retrovirus Research Symposium, Glasgow, 28.-30.05.1998. Fourth International Feline Retrovirus Research Symposium, Glasgow, 28.-30.05.1998.

Klein, D (1998): Real-time PCR: Eine neue Methode zur Virusquantifizierung. 17. Arbeits- und Fortbildungstagung des AVID, Kloster Banz, 23.-25.09.98. 17. Arbeits- und Fortbildungstagung des AVID, Kloster Banz, 23.-25.09.98.

Klein, D (1999): Molecular Epidemiology of FIV: Implications for Virus Quantification and Vaccination Stratgies. The Infectious Disease Review 1,(4).

Quintino, L; Steinrigl, A; Klein, D (2004): Function of ORF A in the context of Feline Immunodeficiency Virus vector development. 7th International Feline Retrovirus Research Symposium, Pisa, 11. - 15.09.2004. 7th International Feline Retrovirus Research Symposium, Pisa, 11. - 15.09.2004.

Steinrigl, A; Quintino, L; Klein, D (2004): Factors involved in transduction of non-dividing cells by Feline Immunodeficiency Virus. 7th International Feline Retrovirus Research Symposium, Pisa, 11. - 15.09.2004. 7th International Feline Retrovirus Research Symposium, Pisa, 11. - 15.09.2004.

Huebner, J; Klein, D; Müller, E; Vahlenkamp, T.W; Langbein, I (2004): Long-Term Combined Anti-Retroviral Therapy (CART) in Feline Immunodeficiency Virus Infected Cats: A Case Report. 7th International Feline Retrovirus Research Symposium, Pisa, 11. - 15.09.2004. 7th International Feline Retrovirus Research Symposium, Pisa, 11. - 15.09.2004.

Haining, H; McMonagle, E.L; Knapp, E; Klein, D; Willett, B.J; Hosie, M.J (2004): Studies on the pathogenicity of primary feline immunodeficiency virus isoltates from different stages of infection. 7th International Feline Retrovirus Research Symposium, Pisa, 11. - 15.09.2004. 7th International Feline Retrovirus Research Symposium, Pisa, 11. - 15.09.2004.

Ryan, G; Klein, D; Brankin, B; McQuaid, S; Hosie, M.J; Callanan, J.J (2004): Assessment of blood-brain barrier integrity within FIV infected brain using confocal microscopy. 7th International Feline Retrovirus Research Symposium, Pisa, 11. - 15.09.2004. 7th International Feline Retrovirus Research Symposium, Pisa, 11. - 15.09.2004.

Klein, D; Schmoll, F; Sipos, W; Indik, S (2004): Genetische Variabilität von PRRSV in Österreich: Konsequenzen für molekulare Diagnostik und Virusquantifizierung. 23. AVID-Tagung, Kloster Banz / Bad Staffelstein, 15.-17.09.2004. 23. AVID-Tagung, Kloster Banz / Bad Staffelstein, 15.-17.09.2004.

Steinrigl, A; Klein, D (2004): Impact of high genetic variability of Feline Immunodeficiency Virus on molecular detection and anti-viral strategies. IN: Recent Research Developments in Virology. Kerala, India, Transworld Research Network, pp. 13-28.

Huebner, J; Klein, D; Müller, E; Vahlenkamp, TW; Langbein, I (2004): Kombinierte anti-retrovirale Therapie (CART) bei einer mit dem Felinen Immunschwächevirus (FIV)-infizierten Katze. Kleintier-Praxis (49), 8 517-524.

Huebner, J; Klein, D; Müller, E; Vahlenkamp, T.W; Langbein, I (2004): Long-Term Combined Anti-Retroviral Therapy (CART) in Feline Immunodeficiency Virus Infected Cats: A Case Report. 22nd Annual ACVIM Forum, Minneapolis, MN, United States (USA), JUN 9-12, 2004. Journal of Veterinary Internal Medicine (18), 3 430-430.

Blacklaws, BA; Berriatua, E; Torsteinsdottir, S; Watt, NJ; de Andres, D; Klein, D; Harkiss, GD (2004): Transmission of small ruminant lentiviruses. Vet Microbiol. 2004; 101(3):199-208

Klein, D; Leichsenring, B; Nosek, D; Knapp, E; Salmons, B; Günzburg, W.H (2004): Replication Kinetics of Retroviral Integrase Mutants and Influence of CIS-Acting Elements. 29. Jahrestagung der ÖGHMP, Bad Ischl, Austria, 24.-27.05.2004. 29. Jahrestagung der ÖGHMP, Bad Ischl, 24.-27.05.2004.

Klein, D. Brunner, B; Steinrigl, A (2004): Implications of molecular evolution on viral quantification using real-time PCR based assays. 1st International qPCR Symposium, Freising, Germany, Germany, 03.-06.03.2004. 1st International qPCR Symposium, Freising, Germany, 03.-06.03.2004.

Klein, D. Nosek, D; Leichsenring, B; Knapp, E (2004): Dissection of the retroviral life cycle using real-time PCR assays. 1st International qPCR Symposium, Freising, germany, Germany, 03.-6.03.2004. 1st International qPCR Symposium, Freising, germany, 03.-6.03.2004.

Klein D (2004): Production of Novel Drugs from Microalgae, Aquatic and Terrestrial Plants with Broad Activity on Several Human, Animal and Plant Viruses Screening and testing systems of natural antiviral substances against animal viruses. EU-Workshop Antivirals, Erlangen, Germany, Germany, 30-31.01.2004. EU-Workshop Antivirals, Erlangen, Germany, 30-31.01.2004.

Klein, D; Steinrigl, A; Knapp, E; Brunner, B; Holzweber, A; Leichsenring, B; Nosek, D; Salmons, B; Günzburg, W.H (2004): Molecular epidemiology and replication kinetics of retroviruses. Inaugural Symposium of the new Christian Doppler Laboratory for Gene Therapeutic Vector Development, Wien, Austria, 22.-24.01.2004. Inaugural Symposium of the new Christian Doppler Laboratory for Gene Therapeutic Vector Development, Wien, 22.-24.01.2004.

Steinrigl, A (2003): Phylogenetische Analyse des Felinen Immundefizienzvirus in Zentraleuropa. Dissertation, Vet. Med. Univ. Wien, pp. 64.

Klein, D (2003): Virus-Übertragungsmechanismen von Tier zu Mensch. Apherese Seminar, Vomp, 10.-13.11.2003. Apherese Seminar, Vomp, 10.-13.11.2003.

Aurich, C; Budik, S; Walter, I; Klein, D; Pittner, F (2003): Untersuchungen zur Charakterisierung der frühembryonalen Genexpression beim Pferd. Uni Vet Wien Report (2003), 3 1-2.

Haining, H; Knapp, E; Willett, B; Klein, D; Hosie, M (2003): Studies of the pathogeniciety of primary isolates of Feline Immunodeficency Virus from cats at different stages of disease. 15th International Workshop on Retroviral Pathogenesis, Glasgow, UK, United Kingdom, 17.-21.09.2003. 15th International Workshop on Retroviral Pathogenesis, Glasgow, UK, 17.-21.09.2003.

Dunham, S; Klein, D; Bruce, J; Golder, M; Jarrett, O; Neil, J.C (2003): Prime boost vaccination against Feline Immunodeficiency Virus. 15th International Workshop on Retroviral Pathogenesis, Glasgow, UK, United Kingdom, 17.-21.09.2003. 15th International Workshop on Retroviral Pathogenesis, Glasgow, UK, 17.-21.09.2003.

Steinrigl, A; Hinterreiter, S; Hohenadl, C; Günzburg, W.H; Klein, D (2003): Development of Improved Models to Study Transduction of Non-dividing Cells by Feline Immunodeficiency Virus. 15 th international workshop on retroviral pathogenesis 2003, Glasgow, UK , United Kingdom, 17-21.09.2003. 15 th international workshop on retroviral pathogenesis 2003, Glasgow, UK , 17-21.09.2003.

Klein, D; Steinrigl, A (2003): Phylogeny of feline immunodeficiency virus in Central Europe: a prerequisite for vaccination and molecular diagnostics. 6th International Congress of Veterinary Virology, St. Malo, Frankreich, France, 24.-27.08.2003. 6th International Congress of Veterinary Virology, St. Malo, Frankreich, 24.-27.08.2003.

Steinrigl, A; Klein D (2003): Phylogenetic analysis of feline immunodeficiency virus as a prerequisite for lentiviral vaccination strategies. Eighth European Workshop on Virus Evolution and Molecular Epidemiology, Leuven, Belgium, Belgium, SEP 4-11, 2002. Infection, Genetics and Evolution (2), 3 219-219.

Ryan, G; Klein, D; Knapp, E; Hosie, MJ; Grimes, T; Mabruk, MJ; Jarrett, O; Callanan, JJ (2003): Dynamics of viral and proviral loads of feline immunodeficiency virus within the feline central nervous system during the acute phase following intravenous infection. J Virol (77), 13 7477-7485.
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Steinborn, R; Müller, M; Brem, G; Klein, D (2003): Quantification by Inhibition of Amplification.

Steinrigl, A; Klein, D (2003): Phylogeny of feline immunodeficiency virus in Central Europe: a prerequisite for vaccination and molecular diagnostics. Jahrestagung der Gesellschaft für Virologie, Berlin, Germany, 26.3. - 29.3.2003. Jahrestagung der Gesellschaft für Virologie, Berlin, 26.3. - 29.3.2003.

Leichsenring, B; Knapp, B; Salmons, B; Günzburg, W. H; Klein, D (2003): Influence of cis-acting elements on the transduction efficiency of retroviral vectors. Jahrestagung der Gesellschaft für Virologie 2003 , Berlin, Germany, 26.3.-29.3.2003. Jahrestagung der Gesellschaft für Virologie 2003 , Berlin, 26.3.-29.3.2003.

Steinrigl, A; Klein, D (2003): Phylogenetic analysis of feline immunodeficiency virus in Central Europe: a prerequisite for vaccination and molecular diagnostics. J Gen Virol. 2003; 84(Pt 5):1301-1307
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Klein, D; Janda, P; Hartmann, K; Salmons, B; Günzburg, WH (1999): Influence of mismatches on viral load determination by real-time PCR. 9th European Congress of Clinical Microbiology and Infectious Diseases, Berlin , Germany, MAR 21–24, 1999. Clinical Microbiology Infection (5), Suppl.3 209-210.

Klein, D (2000): Quantitative Molekularvirologie: Von der Diagnose zur Gentherapie. Habilitationsschrift, Vet. Med. Univ. Wien, pp. 150.

Rungaldier, S; Froeschl, N; Knapp, E; Krassnig, R; Klein, D (2000): Comparison of clinical signs and proviral load by Maedi/Visna specific real-time PCR. International Conference and Workshop on Animal Retroviruses, Queen's College, Cambridge, 2002. International Conference and Workshop on Animal Retroviruses, Queen's College, Cambridge, 2002.

Klein, D; Günzburg, W. H; Salmons, B (2000): Influence of mutations in the integrase on the kinetics of the retroviral life cycle. Jahrestagung der Gesellschaft für Virologie, Wien, Austria, 2000. Jahrestagung der Gesellschaft für Virologie, Wien, 2000.

Klein, D; Salmons, B; Günzburg, W. H (2000): Influence of pre-assay and sequence variations on lentiviral load determination by a multiplex real-time PCR. Jahrestagung der Gesellschaft für Virologie, Wien, Austria, 2000. Jahrestagung der Gesellschaft für Virologie, Wien, 2000.

Klein, D (2002): How retroviruses ensure expression of their genes. Trends in Molecular Medicine (8) 509-509.

Klein, D (2002): NK cells as a reservoir for HIV-1. Trends in Molecular Medicine (8) 414-414.

Klein, D (2002): New pathway for nuclear entry of HIV-1. Trends in Molecular Medicine (8) 55-55.

Klein, D (2002): Quantification using real-time PCR technology: applications and limitations. Trends Mol Med. 2002; 8(6):257-260

Hosie, MJ; Willett, BJ; Klein, D; Dunsford, TH; Cannon, C; Shimojima, M; Neil, JC; Jarrett, O (2002): Evolution of replication efficiency following infection with a molecularly cloned feline immunodeficiency virus of low virulence. J Virol. 2002; 76(12):6062-6072
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Hosie, MJ; Dunsford, T; Klein, D; Willett, BJ; Cannon, C; Osborne, R; Macdonald, J; Spibey, N; Mackay, N; Jarrett, O; Neil, JC (2000): Vaccination with inactivated virus but not viral DNA reduces virus load following challenge with a heterologous and virulent isolate of feline immunodeficiency virus. J Virol. 2000; 74(20):9403-9411
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Indik, S; Valícek, L; Klein, D; Klánová, J (2000): Variations in the major envelope glycoprotein GP5 of Czech strains of porcine reproductive and respiratory syndrome virus. J Gen Virol (81), Pt 10 2497-2502.
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Klein, D; Musil, C; Hirt, R; Gold, P; Thalhammer, JG; Günzburg, WH (2000): Möglichkeiten und Grenzen neuer molekularer Untersuchungsmethoden in der klinischen Mikrobiologie: Dargestellt am Beispiel des Felinen Immundefizienzvirus. Wiener Tierärztliche Monatsschrift (87) 269-277.

Leutenegger, CM; Klein, D; Hofmann-Lehmann, R; Mislin, C; Hummel, U; Böni, J; Boretti, F; Guenzburg, WH; Lutz, H (1999): Rapid feline immunodeficiency virus provirus quantitation by polymerase chain reaction using the TaqMan fluorogenic real-time detection system. J Virol Methods. 1999; 78(1-2):105-116

Klein, D; Janda, P; Steinborn, R; Müller, M; Salmons, B; Günzburg, WH (1999): Proviral load determination of different feline immunodeficiency virus isolates using real-time polymerase chain reaction: influence of mismatches on quantification. Electrophoresis. 1999; 20(2):291-299

Klein, D (2001): Multiplex real-time PCR: Applications and limitations. eingeladener Gastvortrag an der Universität Pisa, Pisa, IT, 9.07.2001 . eingeladener Gastvortrag an der Universität Pisa, Pisa, IT, 9.07.2001 .

Klein, D (2001): Multiplex Real-time PCR: Applications and limitations. Real-time PCR Seminarserie 2001 , Wien, AT, Austria, 1.03.2001 . Real-time PCR Seminarserie 2001 , Wien, AT, 1.03.2001 .

Ryan, G; Klein, D; Callanan, S; Hosie, M; Mabruk, MJEMF; Grimes, T (2001): Viral loads in the feline brain infected with feline immunodeficiency virus: A model for HIV-1 neuropathogenesis. Irish Journal of Medical Science (170), Suppl. 2 76-76.

Klein, D (2001): Filoviruses: new cofactor for cell entry. Trends in Molecular Medicine(Commentary) (7), 10 433-433.

Klein, D (2001): HIV latency in naïve T cells. Trends in Molecular Medicine(Commentary) (7), 7 285-285.

Klein, D (2001): Benefits from early treatment of HIV-1 infection. Trends Mol Med. 2001; 7(1):9

Klein, D; Leutenegger, CM; Bahula, C; Gold, P; Hofmann-Lehmann, R; Salmons, B; Lutz, H; Gunzburg, WH (2001): Influence of preassay and sequence variations on viral load determination by a multiplex real-time reverse transcriptase-polymerase chain reaction for feline immunodeficiency virus. J Acquir Immune Defic Syndr. 2001; 26(1):8-20

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