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Detection of porcine circovirus type 2 by moleculargenetic methods (PCR and ISH)

Abstract
Postweaning multisystemic wasting syndrome (PMWS) is an apparently new disease of weaning and fattening pigs being diagnosed with increasing frequency all over the world. The infectious agent of this disease is porcine circovirus type 2 (PCV-2). Until recently, several methods have been used for the detection of PCV such as virus isolation in cell culture, electron microscopy, in situ hybridization, immunohistochemical staining etc.
The aim of this study is to develope a PCR assay and ISH method for detection and typing of PCV in clinical samples. By this methods we are able to diagnose PMWS according to the definition of Sorden, 2000, that requires that a pig /group of pigs exhibit all of the following:
clinical signs: wasting/weight loss/ill thrift/failure to thrive, with or without dyspnoea or icterus;
histologic lesions: depletion of lymphoid organs/tissues and/or lymphohistiocytic to granulomatous inflammation in any organ (typically lungs and/or lymphoid tissue, and less often liver, kidney, pancreas, intestine);
PCV2 infection within characteristic lesions.
Coordination for vetmeduni vienna
Schmoll Friedrich
Duration
20.01.01-01.12.02
Type of Research
Applied research
Vetmed Research Units
Institute of Pathology
Funded by
5 Publications

Schmoll, F; Sipos, W (2003): Porcine Dermatitis- und Nephropathiesyndrom. VÖS Magazin (2003), 1 8-9.

Opriessnig, T (2002): Untersuchungen, Nachweismethoden, Prävalenz und Sequenzierung porziner Circoviren in Österreich. Dissertation, Vet. Med. Univ. Wien, pp. 250.

Schmoll, F; Sipos, W; Weissenböck, H; Schilcher, F; Schuh, M (2003): Erstmalige Beschreibung des Porcinen Dermatitis und Nephropathie Syndroms (PDNS) in einem österreichischen Schweinebetrieb. Veterinary Medicine Austria / Wiener Tierärztliche Monatsschrift (90) 23-27.

Schilcher, F; Schmoll, F; Rebel-Bauder, B; Url, A; Weissenböck, H; Schmidt, P (2002): Vorkommen von PMWS in Österreich - Pathomorphologische und molekularbiologische Befunde sowie Probleme in der Diagnostik. 45. Tagung der Fachgruppe Pathologie der Deutschen Veterinärmedizinischen Gesellschaft, Wien, Austria, APR 2-3, 2002. Berl Munch Tierarztl Wochenschr (115), 11 474-474.

Schmoll, F; Opriessnig, T; Schilcher, F; Leeb, B; Bauder, B; Schuh, M (2002): Erstmalige Beschreibung des "Postweaning multisystemic wasting syndrome" (PMWS) in einem österreichischen Schweinebetrieb. Wiener Tierärztliche Monatsschrift (89) 50-56.

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