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Investigation of Biochemical and Genetical Diversity of Terpenoid Biosynthesis for the Production of High Value-Added Compounds

Abstract
Terpene synthases are responsible for a key biosynthetic step in the formation of many mono- and sesquiterpenes produced by aromatic plants that are valuable in medicine, in food and in the cosmetic industry.
The overall objective of the project is to exploit the natural diversity of the expression of terpene synthases to obtain a detailed understanding of the molecular and structural basis of terpene synthase function, thus enabling a knowledge-based development of strategies for producing important value-added terpenoids at high levels both in vivo and in vitro.
The terpene synthase genes of three species of sage (Salvia) and oregano (Origanum) will be cloned and expressed, the encoded enzymes characterised, and the role of these catalysts in producing diverse terpene profiles investigated. The elucidation of structure-function relationships within terpene synthase families will provide the theoretical basis for developing the possibility of protein engineering.
Project leader
Duration
01.12.2002-01.12.2005
Type of Research
Basic research

Further information: http://www.vu-wien.ac.at/i104/intesy/

Vetmed Research Units
Institute of Animal Nutrition and Functional Plant Compounds
Projekt partner
Contact: Dipl.-Ing. Dr. Buchtela Georg, MBA
Austria Wirtschaftsservice, Ungargasse 37, A Wien, Austria
Dr. Junghanns GmbH, Untere Dorfstraße 8, D Groß-Schierstedt, Germany
Max Planck Institut für Chemische Ökologie, Winzerlaer Str. 10, D Jena, Germany
University Reading, School of Plant Sciences, Whiteknights, GB Reading, United Kingdom
Vioryl S.A., Viltaniotis Street, 36, GR Athen, Greece
Funded by
EU (Kommission der Europäischen Union), Rue de la Loi, Brussels, European Union
10 Publications

Novak, J (2007): Einfluss der Umwelt auf Ertrag und Qualität von Oregano und Salbei. 17. Bernburger Winterseminar für Arznei- und Gewürzpflanzen; 20.-21.02.2007; Bernburg-Strenzfeld. 2007.

Novak, J; Franz, Ch; Johnson, C.B; Degenhardt, J; Goodenough, P; Ragoussis, N; Junghanns, W; Buchtela, G (2006): QLK3-CT-2002-01930 Investigation of Biochemical and Genetical Diversity of Terpenoid Biosynthesis for the Production of High Value-Added Compounds. Wien, Eigenverlag, pp. 12.

Novak, J; Marn, M; Franz, C (2006): An alpha-pinene chemotype in Salvia officinalis L. (Lamiaceae). Journal of Essential Oil Research (18) 239-241.

Novak, J; Bitsch, C; Marn, M; Franz, C (2005): Blühinduktion bei Echtem Salbei (Salvia officinalis L.). Zeitschrift für Arznei- und Gewürzpflanzen (10), 2 98-99.

Novak, J; Merza-Bahoo, L; Mitteregger, U; Franz, Chl (2005): Composition of the essential oil within summer savory (Satureja hortensis L., Lamiaceae) from syria based on SPME single gland analysis. 36th International Symposium on Essential Oil Research, Budapest, Ungarn, 04.-07.09.2005. 36th International Symposium on Essential Oil Research, Budapest, Ungarn, 04.-07.09.2005.

Johnson, CB; Kazantzis, A; Skoula, M; Mitteregger, U; Novak, J (2004): Seasonal, populational and ontogenic variation in the volatile oil content and composition of individuals of Origanum vulgare subsp. Hirtum, assessed by GC headspace analysis and by SPME sampling of individual oil glands. Phytochem Anal. 2004; 15(5):286-292

Grassi, P; Novak, J; Steinlesberger, H; Franz, C (2004): A direct liquid, non-equilibrium solid-phase micro-extraction application for analysing chemical variation of single peltate trichomes on leaves of Salvia officinalis. Phytochem Anal. 2004; 15(3):198-203

Novak, J (2002): Enantiomere und deren Bedeutung in Komponenten des ätherischen Öles von Gewürzen. Symposium Aktuelle Aspekte der Technologie von Kräutern und Gewürzen II, Gesellschaft Deutscher Lebensmitteltechnologen, Giessen, Deutschland, 23.-25.9.2002. Symposium Aktuelle Aspekte der Technologie von Kräutern und Gewürzen II, Gesellschaft Deutscher Lebensmitteltechnologen, Giessen, Deutschland, 23.-25.9.2002.

Novak, J (2003): Züchterische Erfahrung mit Oregano. Züchtung von Arznei- und Gewürzpflanzen mit antimikrobiellem und antioxidativem Potenzial, Quedlinburg, Deutschland, 30.10.2003. Züchtung von Arznei- und Gewürzpflanzen mit antimikrobiellem und antioxidativem Potenzial, Quedlinburg, Deutschland, 30.10.2003.

Novak, J (2003): Antioxidative Variabilität verschiedener Herkünfte aus der Gattung Origanum. Züchtung von Arznei- und Gewürzpflanzen mit antimikrobiellem und antioxidativem Potenzial, Quedlinburg, Deutschland, 30.10.2003. Züchtung von Arznei- und Gewürzpflanzen mit antimikrobiellem und antioxidativem Potenzial, Quedlinburg, Deutschland, 30.10.2003.

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