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Gewählte Publikation:

Publikationstyp: Zeitschriftenaufsatz
Dokumentart: Originalarbeit

Publikationsjahr: 1998

AutorInnen: Santl, B; Wenigerkind, H; Schernthaner, W; Mödl, J; Stojkovic, M; Prelle, K; Holtz, W; Brem, G; Wolf, E

Titel: Comparison of ultrasound-guided vs laparoscopic transvaginal ovum pick-up (OPU) in simmental heifers.

Quelle: Theriogenology (50), 1 89-100.



Autor/innen der Vetmeduni Vienna:

Brem Gottfried

Beteiligte Vetmed-Organisationseinheiten
Institut für Tierzucht und Genetik


Abstract:
Transvaginal ovum pick-up (OPU) offers several advantages over standard embryo transfer procedures. For a systematic comparison of the ultrasound-guided (U-OPU) and the laparoscopic OPU (L-OPU) method, groups of Simmental heifers were subjected to 1 of the 2 OPU-methods for 8 wk (15 sessions) followed by a treatment-free interval of 11 wk and then another 8-wk period of OPU using the alternative method. Parameters that were evaluated included the number of follicles aspirated, number and morphology of recovered cumulus-oocyte complexes (COCs), and developmental capacity of oocytes after in vitro maturation (IVM) and fertilization (IVF). Blood samples were also taken from the donors twice a wk for progesterone measurement. To evaluate effects of long-term OPU on subsequent fertility of donors, all heifers (n=14) were inseminated during the first natural estrus after the OPU experiment. The proportion of Class I oocytes was significantly (P<0.001) higher after U-OPU than after L-OPU (38.7% vs 21.0%). Following IVM/IVF, this difference in oocyte quality was reflected by the cleavage rate (U-OPU: 58.1%; L-OPU: 52.1%; P<0.05) and the rate of development to morulae and blastocysts (U-OPU: 27.1%; L-OPU: 13.9%; P<0.001). Among other factors, the greater changes in vacuum pressure during L-OPU vs U-OPU might be responsible for the difference in oocyte quality. This problem may be overcome by a more flexible system for regulating the vacuum. Progesterone levels were higher during the L-OPU than the U-OPU periods. Seven donors (50%) were diagnosed pregnant by ultrasonography on Day 28 and by palpation per rectum on Day 42. (C) 1998 by Elsevier Science Inc.

Keywords Pubmed: Animals - : Cattle - : Female - : Fertility - : Laparoscopy - methods : Ovum - ultrasonography : Pregnancy - ultrasonography : Tissue and Organ Harvesting - methods

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