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Gewählte Publikation:

Publikationstyp: Zeitschriftenaufsatz
Dokumentart: Originalarbeit

Publikationsjahr: 2010

AutorInnen: Rütgen, BC; Hammer, SE; Gerner, W; Christian, M; de Arespacochaga, AG; Willmann, M; Kleiter, M; Schwendenwein, I; Saalmüller, A

Titel: Establishment and characterization of a novel canine B-cell line derived from a spontaneously occurring diffuse large cell lymphoma.

Quelle: Leuk Res. 2010; 34(7):932-938



Autor/innen der Vetmeduni Vienna:

Christian Maria
Gerner Wilhelm
Guija-De-Arespacochaga Abigail
Hammer Sabina
Kleiter Miriam
Rütgen Barbara
Saalmüller Armin
Schwendenwein Ilse
Willmann Michael

Beteiligte Vetmed-Organisationseinheiten
Institut für Immunologie
Universitätsklinik für Kleintiere, Klinische Abteilung für Interne Medizin Kleintiere
Plattform Labordiagnostik


Abstract:
Cell lines derived from spontaneous tumors serve as a research tool for cancer cell biology and new anti-cancer drug development. Isolation and propagation of canine lymphoma cell lines is difficult, thus only a few are available. Now we have established a new B-cell lymphoma cell line CLBL-1 from a dog with confirmed stage IV diffuse large cell lymphoma. Immunophenotyping of these CLBL-1 cells showed positive staining for CD11a, CD79alphacy, CD45, CD45RA, MHC II and cells were negative for CD3, CD4, CD5, CD8, CD11d, CD14, CD21, CD34, CD56 and T-cell receptor-gammadelta (TCR-gammadelta). PCR analysis for TCR-gamma and immunoglobulin heavy chain (IgH) gene rearrangements yielded a monoclonal result for the IgH gene. Furthermore, the clonality of IgH gene rearrangement was confirmed by sequencing of 16 positive bacterial clones. As canine lymphoma resembles non-Hodgkin"s lymphoma (NHL) in humans in many respects, this new cell line, will promote translational and comparative lymphoma research in humans and dogs.

Keywords Pubmed: Animals
Antigens, CD/analysis
Antigens, Neoplasm/analysis
B-Lymphocytes/chemistry
B-Lymphocytes/pathology*
Biopsy, Fine-Needle
Cell Line, Tumor/chemistry
Cell Line, Tumor/pathology*
Cell Separation
Clone Cells/chemistry
Clone Cells/pathology
Dog Diseases/pathology*
Dogs
Flow Cytometry
Gene Rearrangement, B-Lymphocyte, Heavy Chain
Immunophenotyping
Lymph Nodes/pathology
Lymphoma, Large B-Cell, Diffuse/pathology
Lymphoma, Large B-Cell, Diffuse/veterinary*
Male
Neoplasm Proteins/analysis
Receptors, Antigen, T-Cell, gamma-delta/analysis


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