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Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Originalarbeit

Jahr: 2013

AutorInnen: Reutner, K; Leitner, J; Müllebner, A; Ladinig, A; Essler, SE; Duvigneau, JC; Ritzmann, M; Steinberger, P; Saalmüller, A; Gerner, W

Titel: CD27 expression discriminates porcine T helper cells with functionally distinct properties.

Quelle: Vet Res. 2013; 44:18

Autor/innen der Vetmeduni Vienna:

Duvigneau Catharina
Gerner Wilhelm
Hammer Sabina
Ladinig Andrea
Müllebner Andrea
Reutner Katharina
Ritzmann Mathias
Saalmüller Armin

Beteiligte Vetmed-Organisationseinheiten
Institut für Immunologie
Institut für Medizinische Biochemie
Universitätsklinik für Schweine

Differentiation of porcine T helper cells is still poorly investigated, partly due to a lack of monoclonal antibodies (mAbs) specific for molecules involved in this process. Recently, we identified a mAb specific for porcine CD27 and showed that CD27 is expressed by all naïve CD8α- T helper cells but divides CD8α+ T helper cells into a CD27+ and a CD27- subset. In the present study, detailed phenotypical and functional analyses of these T-helper cell subpopulations were performed. Naïve CD8α-CD27+ T helper cells predominantly resided in various lymph nodes, whereas higher proportions of CD8α+CD27+ and CD8α+CD27- T helper cells were found in blood, spleen and liver. Both CD8α+CD27+ and CD8α+CD27- T helper cells were capable of producing IFN-γ upon in vitro polyclonal stimulation and antigen-specific restimulation. Experiments with sorted CD8α-CD27+, CD8α+CD27+ and CD8α+CD27- T-helper cell subsets following polyclonal stimulation revealed the lowest proliferative response but the highest ability for IFN-γ and TNF-α production in the CD8α+CD27- subset. Therefore, these cells resembled terminally differentiated effector memory cells as described in human. This was supported by analyses of CCR7 and CD62L expression. CD8α+CD27- T helper cells were mostly CCR7- and had considerably reduced CD62L mRNA levels. In contrast, expression of both homing-receptors was increased on CD8α+CD27+ T helper cells, which also had a proliferation rate similar to naïve CD8α-CD27+ T helper cells and showed intermediate levels of cytokine production. Therefore, similar to human, CD8α+CD27+ T helper cells displayed a phenotype and functional properties of central memory cells.

Keywords Pubmed: Animals
Antigens, CD27/chemistry
Antigens, CD27/genetics
Antigens, CD27/immunology*
Cell Differentiation
Lymphocyte Activation*
Molecular Sequence Data
Sequence Alignment/veterinary
Sequence Analysis, Protein/veterinary
Sus scrofa/immunology*
T-Lymphocyte Subsets/cytology
T-Lymphocyte Subsets/immunology
T-Lymphocyte Subsets/metabolism

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