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Gewählte Publikation:

Publikationstyp: Zeitschriftenaufsatz
Dokumentart: Originalarbeit

Publikationsjahr: 2015

AutorInnen: Metzler-Zebeli, BU; Mann, E; Ertl, R; Schmitz-Esser, S; Wagner, M; Klein, D; Ritzmann, M; Zebeli, Q

Titel: Dietary calcium concentration and cereals differentially affect mineral balance and tight junction proteins expression in jejunum of weaned pigs.

Quelle: Br J Nutr. 2015; 113(7):1019-1031



Autor/innen der Vetmeduni Vienna:

Ertl Reinhard
Klein Dieter
Mann-Selberherr Evelyne
Metzler-Zebeli Barbara
Schmitz-Esser Stephan
Wagner Martin
Zebeli Qendrim

Beteiligte Vetmed-Organisationseinheiten
Abteilung für Lebensmittelmikrobiologie
Institut für Tierernährung und funktionelle Pflanzenstoffe
Universitätsklinik für Schweine
VetCore


Abstract:
Ca plays an essential role in bone development; however, little is known about its effect on intestinal gene expression in juvenile animals. In the present study, thirty-two weaned pigs (9·5 (SEM 0·11) kg) were assigned to four diets that differed in Ca concentration (adequate v. high) and cereal composition (wheat-barley v. maize) to assess the jejunal and colonic gene expression of nutrient transporters, tight junction proteins, cytokines and pathogen-associated molecular patterns, nutrient digestibility, Ca balance and serum acute-phase response. To estimate the impact of mucosal bacteria on colonic gene expression, Spearman"s correlations between colonic gene expression and bacterial abundance were computed. Faecal Ca excretion indicated that more Ca was available along the intestinal tract of the pigs fed high Ca diets as compared to the pigs fed adequate Ca diets (P> 0.05). High Ca diets decreased jejunal zonula occludens 1 (ZO1) and occludin (OCLN) expression, up-regulated jejunal expression of toll-like receptor 2 (TLR2) and down-regulated colonic GLUT2 expression as compared to the adequate Ca diets (P< 0.05). Dietary cereal composition up-regulated jejunal TLR2 expression and interacted (P= 0.021) with dietary Ca on colonic IL1B expression; high Ca concentration up-regulated IL1B expression with wheat-barley diets and down-regulated it with maize diets. Spearman"s correlations (r> 0·35; P< 0·05) indicated an association between operational taxonomic units assigned to the phyla Bacteroidetes, Firmicutes and Proteobacteria and bacterial metabolites and mucosal gene expression in the colon. The present results indicate that high Ca diets have the potential to modify the jejunal and colonic mucosal gene expression response which, in turn, interacts with the composition of the basal diet and mucosa-associated bacteria in weaned pigs.

Keywords Pubmed: Animal Feed*/analysis
Animals
Austria
Calcium, Dietary/administration & dosage*
Calcium, Dietary/analysis
Castration/veterinary
Colon/growth & development
Colon/metabolism
Colon/microbiology
Crosses, Genetic
Feces/chemistry
Feces/microbiology
Gene Expression Regulation, Developmental*
Glucose Transporter Type 2/genetics
Glucose Transporter Type 2/metabolism
Gram-Negative Bacteria/growth & development
Gram-Negative Bacteria/isolation & purification
Gram-Negative Bacteria/metabolism
Gram-Positive Bacteria/growth & development
Gram-Positive Bacteria/isolation & purification
Gram-Positive Bacteria/metabolism
Hordeum/chemistry
Intestinal Mucosa/growth & development
Intestinal Mucosa/metabolism*
Intestinal Mucosa/microbiology
Jejunum/growth & development
Jejunum/metabolism*
Jejunum/microbiology
Male
Sus scrofa/growth & development
Sus scrofa/microbiology
Sus scrofa/physiology*
Tight Junction Proteins/genetics
Tight Junction Proteins/metabolism*
Toll-Like Receptor 2/genetics
Toll-Like Receptor 2/metabolism
Triticum/chemistry
Weaning
Zea mays/chemistry


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