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Gewählte Publikation:

Publikationstyp: Zeitschriftenaufsatz
Dokumentart: Originalarbeit

Publikationsjahr: 2004

AutorInnen: Chizzola, R; Hochsteiner, W; Hajek, S

Titel: GC analysis of essential oils in the rumen fluid after incubation of Thuja orientalis twigs in the Rusitec system.

Quelle: Res Vet Sci. 2004; 76(1):77-82



Autor/innen der Vetmeduni Vienna:

Chizzola Remigius
Hochsteiner Werner

Beteiligte Vetmed-Organisationseinheiten
Institut für Tierernährung und funktionelle Pflanzenstoffe
Universitätsklinik für Wiederkäuer, Klinische Abteilung für Wiederkäuermedizin


Abstract:
Methods for the chemical analysis of toxic plant substances in the rumen of ruminants are of importance for the diagnosis of intoxications with poisonous plants. The present work establishes a method to estimate monoterpene components of the essential oil of thuja (Thuja orientalis, Cupressaceae) in these types of samples. Alpha-thujone, which is regarded as the toxic principle, is present at a concentration of 50-60% in the essential oil. The rumen simulation technique (Rusitec) was used to simulate natural digestion. Chopped twigs of thuja were subjected to rumen content in a closed container with an overflow device. The flow of saliva was simulated by the continuous addition of a buffer solution. Samples for analysis were taken from the overflow at 24 and 48 h. A further sample was taken from the remaining liquid fraction of the rumen content in the container at 48 h. The essential oils were extracted with hexane and concentrated. A quantitative determination was done by capillary gas chromatography. Together in the three fractions analysed this resulted in total mean recoveries of 6.8% for alpha-thujone, 5.3% for beta-thujone, 18.9% for fenchone and 27.8% for camphor. The observation that the thujones were recovered to a lesser extent than other oil components is evidence of their fast decomposition in the rumen medium. Under these circumstances the calculated detection limit is 100-200 g thuja twigs in cows with rumen volumes of 60-100 litres. The main essential oil degradation products found in the rumen fluid of all three fractions in the Rusitec system were discovered to be iso-3-thujanol, neo-3-thujanol, carvomenthol and carvomenthone.

Keywords Pubmed: Animals
Cattle
Chromatography, Gas
Digestion
Gas Chromatography-Mass Spectrometry
Models, Biological
Oils, Volatile/analysis*
Oils, Volatile/pharmacokinetics*
Plant Stems/chemistry*
Plants, Toxic*
Rumen/chemistry*
Rumen/secretion*
Thuja/chemistry*


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