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Gewählte Publikation:

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Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Originalarbeit

Jahr: 2011

AutorInnen: Glaschke, A; Weiland, J; Del Turco, D; Steiner, M; Peichl, L; Glösmann, M

Titel: Thyroid hormone controls cone opsin expression in the retina of adult rodents.

Quelle: J Neurosci. 2011; 31(13):4844-4851



Autor/innen der Vetmeduni Vienna:

Glösmann Martin

Beteiligte Vetmed-Organisationseinheiten
VetCore


Abstract:
Mammalian retinas display an astonishing diversity in the spatial arrangement of their spectral cone photoreceptors, probably in adaptation to different visual environments. Opsin expression patterns like the dorsoventral gradients of short-wave-sensitive (S) and middle- to long-wave-sensitive (M) cone opsin found in many species are established early in development and thought to be stable thereafter throughout life. In mouse early development, thyroid hormone (TH), through its receptor TRβ2, is an important regulator of cone spectral identity. However, the role of TH in the maintenance of the mature cone photoreceptor pattern is unclear. We here show that TH also controls adult cone opsin expression. Methimazole-induced suppression of serum TH in adult mice and rats yielded no changes in cone numbers but reversibly altered cone patterns by activating the expression of S-cone opsin and repressing the expression of M-cone opsin. Furthermore, treatment of athyroid Pax8(-/-) mice with TH restored a wild-type pattern of cone opsin expression that reverted back to the mutant S-opsin-dominated pattern after termination of treatment. No evidence for cone death or the generation of new cones from retinal progenitors was found in retinas that shifted opsin expression patterns. Together, this suggests that opsin expression in terminally differentiated mammalian cones remains subject to control by TH, a finding that is in contradiction to previous work and challenges the current view that opsin identity in mature mammalian cones is fixed by permanent gene silencing.

Keywords Pubmed: Age Factors
Animals
Cell Differentiation/genetics
Cell Differentiation/physiology
Cone Opsins/biosynthesis*
Gene Expression Regulation*
Hypothyroidism/metabolism
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
Mice, Transgenic
Paired Box Transcription Factors/biosynthesis
Paired Box Transcription Factors/deficiency
Paired Box Transcription Factors/genetics
Rats
Rats, Inbred BN
Retina/metabolism*
Rod Opsins/biosynthesis*
Thyroid Hormones/physiology*


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