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Gewählte Publikation:

Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Originalarbeit

Jahr: 2005

AutorInnen: Moriggl, R; Sexl, V; Kenner, L; Duntsch, C; Stangl, K; Gingras, S; Hoffmeyer, A; Bauer, A; Piekorz, R; Wang, D; Bunting, KD; Wagner, EF; Sonneck, K; Valent, P; Ihle, JN; Beug, H

Titel: Stat5 tetramer formation is associated with leukemogenesis.

Quelle: Cancer Cell (7), 1 87-99.



Autor/innen der Vetmeduni Vienna:

Kenner Lukas
Moriggl Richard
Sexl Veronika

Diese Publikation wurde nicht im Namen der Vetmeduni Vienna erstellt und ist deshalb ausschließlich der persönlichen Publikationsliste des/der Autors/Autorin zugeordnet!


Abstract:
Activation of Stat5 is frequently found in leukemias. To study the mechanism and role of Stat5 activation, we introduced a constitutively activated Stat5a mutant, cS5F, into murine bone marrow (BM) cells. BM transplantation with cS5F-transfected cells caused development of multilineage leukemias in lethally irradiated wild-type or nonirradiated Rag2(-/-) mice. The leukemic cells showed strongly enhanced levels of cS5F tetramers but unchanged cS5F dimer levels in a DNA binding assay. Moreover, Stat5a mutants engineered to form only dimers, but not tetramers, failed to induce leukemias. In addition, Stat5 tetramers were found to accumulate in excess compared to dimers in various human leukemias. These data suggest that Stat5 tetramers are associated with leukemogenesis.

Keywords Pubmed: Animals
Biomarkers
Bone Marrow Cells/cytology
Bone Marrow Cells/physiology
Bone Marrow Transplantation
Cell Lineage
Cell Transformation, Neoplastic
Cells, Cultured
DNA-Binding Proteins/chemistry*
DNA-Binding Proteins/genetics
DNA-Binding Proteins/metabolism*
Female
Genetic Complementation Test
Growth Substances/metabolism
Humans
Leukemia/genetics
Leukemia/metabolism*
Leukemia/pathology
Liver/metabolism
Liver/pathology
Male
Mice
Mice, Knockout
Milk Proteins/chemistry*
Milk Proteins/genetics
Milk Proteins/metabolism*
Mutation
Nuclear Proteins
Oncogenes
Protein Structure, Quaternary*
STAT5 Transcription Factor
Spleen/metabolism
Spleen/pathology
Trans-Activators/chemistry*
Trans-Activators/genetics
Trans-Activators/metabolism*
Transfection
Tumor Suppressor Proteins


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