Veterinärmedizinische Universität Wien Forschungsinformationssystem VetDoc

Gewählte Publikation:

Open Access Logo

Publikationstyp: Zeitschriftenaufsatz
Dokumentart: Originalarbeit

Publikationsjahr: 2016

AutorInnen: Talker, SC; Stadler, M; Koinig, HC; Mair, KH; Rodríguez-Gómez, IM; Graage, R; Zell, R; Dürrwald, R; Starick, E; Harder, T; Weissenböck, H; Lamp, B; Hammer, SE; Ladinig, A; Saalmüller, A; Gerner, W

Titel: Influenza A Virus Infection in Pigs Attracts Multifunctional and Cross-Reactive T Cells to the Lung.

Quelle: J Virol. 2016; 90(20):9364-9382

Autor/innen der Vetmeduni Vienna:

Gerner Wilhelm
Graage Robert
Hammer Sabina
Koinig Hanna
Ladinig Andrea
Lamp Benjamin
Mair Kerstin
Rodriguez Gomez Irene Magdalena
Saalmüller Armin
Stadler Maria
Talker Stephanie
Weissenböck Herbert

Beteiligte Vetmed-Organisationseinheiten
Institut für Immunologie
Institut für Pathologie
Institut für Virologie
Universitätsklinik für Schweine

Pigs are natural hosts for influenza A viruses and play a critical role in influenza epidemiology. However, little is known about their influenza-evoked T-cell response. We performed a thorough analysis of both the local and systemic T-cell response in influenza virus-infected pigs, addressing kinetics and phenotype as well as multifunctionality (gamma interferon [IFN-γ], tumor necrosis factor alpha [TNF-α], and interleukin-2 [IL-2]) and cross-reactivity. A total of 31 pigs were intratracheally infected with an H1N2 swine influenza A virus (FLUAVsw) and consecutively euthanized. Lungs, tracheobronchial lymph nodes, and blood were sampled during the first 15 days postinfection (p.i.) and at 6 weeks p.i. Ex vivo flow cytometry of lung lymphocytes revealed an increase in proliferating (Ki-67(+)) CD8(+) T cells with an early effector phenotype (perforin(+) CD27(+)) at day 6 p.i. Low frequencies of influenza virus-specific IFN-γ-producing CD4(+) and CD8(+) T cells could be detected in the lung as early as 4 days p.i. On consecutive days, influenza virus-specific CD4(+) and CD8(+) T cells produced mainly IFN-γ and/or TNF-α, reaching peak frequencies around day 9 p.i., which were up to 30-fold higher in the lung than in tracheobronchial lymph nodes or blood. At 6 weeks p.i., CD4(+) and CD8(+) memory T cells had accumulated in lung tissue. These cells showed diverse cytokine profiles and in vitro reactivity against heterologous influenza virus strains, all of which supports their potential to combat heterologous influenza virus infections in pigs.

Keywords Pubmed: Animals
CD4-Positive T-Lymphocytes/immunology*
CD4-Positive T-Lymphocytes/virology
CD8-Positive T-Lymphocytes/immunology*
Cross Reactions/immunology*
Influenza A Virus, H1N2 Subtype/immunology*
Influenza Vaccines/immunology
Orthomyxoviridae Infections/immunology*
Swine Diseases/immunology*
Swine Diseases/virology
Tumor Necrosis Factor-alpha/immunology

© Veterinärmedizinische Universität Wien Hilfe und Downloads