Veterinärmedizinische Universität Wien VetDoc - Forschungsdokumentation

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Publikationstyp: Zeitschriftenaufsatz
Dokumentart: Originalarbeit

Publikationsjahr: 2017

AutorInnen: Riedel, C; Lamp, B; Hagen, B; Indik, S; Rümenapf, T

Titel: The core protein of a pestivirus protects the incoming virus against IFN-induced effectors.

Quelle: Sci Rep. 2017; 7:44459

Autor/innen der Vetmeduni Vienna:

Hagen Benedikt,
Indik Stanislav,
Lamp Benjamin,
Riedel Christiane,
Rümenapf Hans Tillmann,

Beteiligte Vetmed-Organisationseinheiten
Institut für Virologie,

A multitude of viral factors - either inhibiting the induction of the IFN-system or its effectors - have been described to date. However, little is known about the role of structural components of the incoming virus particle in protecting against IFN-induced antiviral factors during or immediately after entry. In this study, we take advantage of the previously reported property of Classical swine fever virus (family Flaviviridae, genus Pestivirus) to tolerate a deletion of the core protein if a compensatory mutation is present in the NS3-helicase-domain (Vp447∆c). In contrast to the parental virus (Vp447), which causes a hemorrhagic-fever-like disease in pigs, Vp447∆c is avirulent in vivo. In comparison to Vp447, growth of Vp447∆c in primary porcine cells and IFN-treated porcine cell lines was reduced >20-fold. Also, primary porcine endothelial cells and IFN-pretreated porcine cell lines were 8-24 times less susceptible to Vp447∆c. This reduction of susceptibility could be partially reversed by loading Vp447∆c particles with different levels of core protein. In contrast, expression of core protein in the recipient cell did not have any beneficial effect. Therefore, a protective effect of core protein in the incoming virus particle against the products of IFN-stimulated genes could be demonstrated.

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