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Publikationstyp: Zeitschriftenaufsatz
Dokumentart: Originalarbeit

Publikationsjahr: 2017

AutorInnen: Gutiérrez, A; Cerón, JJ; Razzazi-Fazeli, E; Schlosser, S; Tecles, F

Titel: Influence of different sample preparation strategies on the proteomic identification of stress biomarkers in porcine saliva.

Quelle: BMC Vet Res. 2017; 13(1):375

Autor/innen der Vetmeduni Vienna:

Razzazi-Fazeli Ebrahim,
Schlosser Sarah,

Beteiligte Vetmed-Organisationseinheiten

The influence of two different sample treatments comprising the enrichment of glycoproteins by boronic acid and dynamic range compression by hexapeptide libraries, on the detection of stress markers in saliva of pigs was evaluated in this study. For this purpose, saliva samples collected before and after the application of an acute stress model consisting of nasal restraining in pigs were processed without any treatment and with the two different treatments mentioned above. Protein separation by two-dimensional gel electrophoresis (2-DE) followed by identification of proteins using MALDI-TOF/TOF mass spectrometry (MS) was used as proteomic technique.

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