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Publikationstyp: Zeitschriftenaufsatz
Dokumentart: Originalarbeit

Publikationsjahr: 2018

AutorInnen: Mester, P; Wagner, M; Rossmanith, P

Titel: Molecular Enrichment for Qualitative Molecular Pathogen Detection in Food.

Quelle: Food Analytical Methods 2018; 11: 1251-1256



Autor/innen der Vetmeduni Vienna:

Mester Patrick-Julian,
Rossmanith Peter,
Wagner Martin,

Beteiligte Vetmed-Organisationseinheiten
Institut für Milchhygiene,


Abstract:
Reliable detection of low levels of pathogenic microbial contaminants is one of the most important prerequisites to guarantee safe food production. In this work, the use of molecular enrichment (ME) subsequent to the sample preparation method Matrix-Lysis was evaluated solely for qualitative molecular pathogen detection from complex matrices. It could be successfully shown that the LOD of an all-molecular pathogen detection approach for food was effectively lowered by a factor of ten from 1.60 x 10(1) CFU/ml to 1.60 x 10(0) CFU/ml due to ME.


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