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Gewählte Publikation:

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Publikationstyp: Zeitschriftenaufsatz
Dokumentart: Originalarbeit

Publikationsjahr: 2001

AutorInnen: Nowshari, MA; Brem, G

Titel: Effect of freezing rate and exposure time to cryoprotectant on the development of mouse pronuclear stage embryos.

Quelle: Hum Reprod (16), 11 2368-2373.



Autor/innen der Vetmeduni Vienna:

Brem Gottfried,

Beteiligte Vetmed-Organisationseinheiten
Institut für Tierzucht und Genetik,


Abstract:
The effects of exposure time (20 versus 45 s) to a high concentration of cryoprotectant (7.0 mol/l ethylene glycol with 0.5 mol/l sucrose) and freezing rates (1200-10 300 degrees C/min) during rapid freezing of mouse pronuclear stage embryos on survival and development to blastocysts were investigated. Different freezing rates were achieved by directly plunging the straws (rapid freezing) and open pulled straws (OPS) in liquid nitrogen (OPS freezing) and by plunging the straws (super rapid) and OPS (super OPS) in a super cooled liquid nitrogen chamber (at -212 degrees C) before storage in liquid nitrogen.

Keywords Pubmed: Animals
Blastocyst/physiology
Cryopreservation/instrumentation
Cryopreservation/methods*
Cryoprotective Agents/administration & dosage*
Ethylene Glycol/administration & dosage
Female
Mice
Mice, Inbred C57BL
Mice, Inbred CBA
Nitrogen
Sucrose/administration & dosage
Time Factors
Zygote/growth & development*


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