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Gewählte Publikation:

Publikationstyp: Zeitschriftenaufsatz
Dokumentart: Originalarbeit

Publikationsjahr: 2002

AutorInnen: Rekkas, CA; Besenfelder, U; Havlicek, V; Vainas, E; Brem, G

Titel: Plasminogen activator activity in cortical granules of bovine oocytes during in vitro maturation.

Quelle: Theriogenology. 2002; 57(7):1897-1905



Autor/innen der Vetmeduni Vienna:

Besenfelder Urban,
Brem Gottfried,

Beteiligte Vetmed-Organisationseinheiten
Institut für Tierzucht und Genetik,
Interuniversitäres Department für Agrarbiotechnologie (IFA),


Abstract:
In this study, we provide evidence that plasminogen activator of tissue-type (t-PA), at least, is present in extracts of bovine oocyte cortical granules, and that its activity varies significantly with the duration of oocyte in vitro maturation. Cortical granules were collected from bovine oocytes by means of micromanipulation, after 0, 12, or 24 h of IVM. Our results show that plasminogen activator activity of cortical granule extracts was significantly higher after 24 h of IVM than after 12 h of IVM or before IVM. This activity was apparently due, at least partly, to tissue-type plasminogen activator as shown immunologically. No evidence was found for the presence of urokinase-type plasminogen activator, plasminogen activator inhibitors or plasmin inhibitors in bovine oocyte cortical granule extracts. Our findings further support the hypothesis that t-PA activity of oocyte origin may have a role in oocyte maturation or fertilization, as well as in post-fertilization events, such as cortical reaction and formation of the zona block to polyspermy.

Keywords Pubmed: Amiloride/pharmacology
Animals
Antifibrinolytic Agents/analysis
Cattle
Enzyme Inhibitors/pharmacology
Female
Oocytes/chemistry*
Oocytes/physiology*
Oocytes/ultrastructure
Plasminogen Inactivators/analysis
Time Factors
Tissue Plasminogen Activator/analysis*
Tissue Plasminogen Activator/immunology
Urokinase-Type Plasminogen Activator/analysis
Urokinase-Type Plasminogen Activator/antagonists & inhibitors


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