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Publikationstyp: Zeitschriftenaufsatz
Dokumentart: Originalarbeit

Publikationsjahr: 2004

AutorInnen: Grosse-Hovest, L; Müller, S; Minoia, R; Wolf, E; Zakhartchenko, V; Wenigerkind, H; Lassnig, C; Besenfelder, U; Müller, M; Lytton, SD; Jung, G; Brem, G

Titel: Cloned transgenic farm animals produce a bispecific antibody for T cell-mediated tumor cell killing.

Quelle: Proc Natl Acad Sci U S A. 2004; 101(18):6858-6863

Autor/innen der Vetmeduni Vienna:

Besenfelder Urban,
Brem Gottfried,
Müller Mathias,
Müller Simone,

Beteiligte Vetmed-Organisationseinheiten
Institut für Tierzucht und Genetik,

Complex recombinant antibody fragments for modulation of immune function such as tumor cell destruction have emerged at a rapid pace and diverse anticancer strategies are being developed to benefit patients. Despite improvements in molecule design and expression systems, the quantity and stability, e.g., of single-chain antibodies produced in cell culture, is often insufficient for treatment of human disease, and the costs of scale-up, labor, and fermentation facilities are prohibitive. The ability to yield mg/ml levels of recombinant antibodies and the scale-up flexibility make transgenic production in plants and livestock an attractive alternative to mammalian cell culture as a source of large quantities of biotherapeutics. Here, we report on the efficient production of a bispecific single-chain antibody in the serum of transgenic rabbits and a herd of nine cloned, transgenic cattle. The bispecific protein, designated r28M, is directed to a melanoma-associated proteoglycan and the human CD28 molecule on T cells. Purified from the serum of transgenic animals, the protein is stable and fully active in mediating target cell-restricted T cell stimulation and tumor cell killing.

Keywords Pubmed: Animals
Cloning, Organism
Jurkat Cells

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