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Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Originalarbeit

Jahr: 2014

AutorInnen: Andrukhova, O; Slavic, S; Zeitz, U; Riesen, SC; Heppelmann, MS; Ambrisko, TD; Markovic, M; Kuebler, WM; Erben, RG

Titel: Vitamin D is a regulator of endothelial nitric oxide synthase and arterial stiffness in mice.

Quelle: Mol Endocrinol. 2014; 28(1):53-64

Autor/innen der Vetmeduni Vienna:

Ambrisko Tamas
Andrukhova Olena
Erben Reinhold
Markovic Mato
Riesen Sabine
Slavic Svetlana
Zeitz Ute

Beteiligte Vetmed-Organisationseinheiten
Institut für Physiologie, Pathophysiologie und Biophysik, Abteilung für Physiologie, Pathophysiologie und experimentelle Endokrinologie
Universitätsklinik für Kleintiere, Klinische Abteilung für Anästhesiologie und perioperative Intensivmedizin
Universitätsklinik für Kleintiere, Klinische Abteilung für Interne Medizin Kleintiere

The vitamin D hormone 1α,25-dihydroxyvitamin D3 [1,25(OH)2D3] is essential for the preservation of serum calcium and phosphate levels but may also be important for the regulation of cardiovascular function. Epidemiological data in humans have shown that vitamin D insufficiency is associated with hypertension, left ventricular hypertrophy, increased arterial stiffness, and endothelial dysfunction in normal subjects and in patients with chronic kidney disease and type 2 diabetes. However, the pathophysiological mechanisms underlying these associations remain largely unexplained. In this study, we aimed to decipher the mechanisms by which 1,25(OH)2D3 may regulate systemic vascular tone and cardiac function, using mice carrying a mutant, functionally inactive vitamin D receptor (VDR). To normalize calcium homeostasis in VDR mutant mice, we fed the mice lifelong with the so-called rescue diet enriched with calcium, phosphate, and lactose. Here, we report that VDR mutant mice are characterized by lower bioavailability of the vasodilator nitric oxide (NO) due to reduced expression of the key NO synthesizing enzyme, endothelial NO synthase, leading to endothelial dysfunction, increased arterial stiffness, increased aortic impedance, structural remodeling of the aorta, and impaired systolic and diastolic heart function at later ages, independent of changes in the renin-angiotensin system. We further demonstrate that 1,25(OH)2D3 is a direct transcriptional regulator of endothelial NO synthase. Our data demonstrate the importance of intact VDR signaling in the preservation of vascular function and may provide a mechanistic explanation for epidemiological data in humans showing that vitamin D insufficiency is associated with hypertension and endothelial dysfunction.

Keywords Pubmed: Animals
Enzyme Induction
Gene Expression
Gene Knockout Techniques
HEK293 Cells
Mice, Inbred C57BL
Mice, Knockout
Nitric Oxide/metabolism
Nitric Oxide Synthase Type III/genetics
Nitric Oxide Synthase Type III/metabolism*
Receptors, Calcitriol/genetics
Receptors, Calcitriol/metabolism
Tissue Culture Techniques
Transcriptional Activation
Vascular Stiffness*
Vitamin D/analogs & derivatives*
Vitamin D/physiology

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