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Gewählte Publikation:

Publikationstyp: Zeitschriftenaufsatz
Dokumentart: Originalarbeit

Publikationsjahr: 2018

AutorInnen: Grilz-Seger, G; Mesarič, M; Cotman, M; Neuditschko, M; Druml, T; Brem, G

Titel: Runs of Homozygosity and Population History of Three Horse Breeds With Small Population Size.

Quelle: Journal of Equine Veterinary Science 2018; 71: 27-34



Autor/innen der Vetmeduni Vienna:

Brem Gottfried,
Druml Thomas,
Grilz-Seger Gertrud,

Beteiligte Vetmed-Organisationseinheiten
Abteilung für Reproduktionsbiologie,


Zugehörige(s) Projekt(e): Automatisation der Phänotypisierungen von Exterieur-und Bewegungsmerkmalen und genomische Analysen beim Lipizzaner


Abstract:
Long consecutive homozygous genotype segments, runs of homozygosity (ROH), are a result of parents transmitting identical haplotypes, which can be used to estimate autozygosity. Based on 612K single-nucleotide polymorphisms, we computed three ROH parameters (genome length covered by ROH, S-ROH; number of ROH, N-ROH; and autozygosity, F-ROH) to investigate different scenarios in contemporary horse breeding: limited census (Bosnian mountain horse), conservation breeding (Posavje horse), and selection within closed studbook (Haflinger). The ROH parameters revealed well-defined differences between breeds. S-ROH was highest in the Bosnian mountain horse with 296.32 Mb, followed by the Haflinger sample (S-ROH = 270.35 Mb) and the Posavje sample with 192.68 Mb. The highest number of ROH segments (ROHs) was observed within the Haflinger sample followed by the Posavje sample. F-ROH ranged at a population level from 8.59% in Posavje, over the Haflinger (mean F-ROH = 12.05%) to 13.21% in the Bosnian mountain horse breed. Bottlenecks were detected for Bosnian mountain horse and Haflinger, whereas for the Posavje, a positive effect of the conservation breeding program was documented. Investigating the distribution of ROHs across the genome, we detected four common ROH islands on equine chromosomes ECA 6, ECA 11, and ECA 17, which were present in all breeds. On breed level, the Bosnian mountain horses contained 10, the Posavje, four, and the Haflinger, 11 distinct ROH islands (containing the MC1R locus on ECA 3). With this analysis, we were able to compare genomic levels of inbreeding between breeds differing in management, pedigree completeness, and genes under selection. (C) 2018 Elsevier Inc. All rights reserved.


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