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Gewählte Publikation:

Publikationstyp: Zeitschriftenaufsatz
Dokumentart: Originalarbeit

Publikationsjahr: 2010

AutorInnen: Klinglmayr, C; Nöbauer, K; Razzazi-Fazeli, E; Cichna-Markl, M

Titel: Determination of deoxynivalenol in organic and conventional food and feed by sol-gel immunoaffinity chromatography and HPLC-UV detection.

Quelle: J Chromatogr B Analyt Technol Biomed Life Sci. 2010; 878(2):187-193



Autor/innen der Vetmeduni Vienna:

Nöbauer Katharina
Razzazi-Fazeli Ebrahim

Beteiligte Vetmed-Organisationseinheiten
Institut für Tierernährung und funktionelle Pflanzenstoffe
VetCore


Abstract:
The paper describes the determination of deoxynivalenol (DON) in 55 wheat food and feed samples, 26 from conventional and 29 from organic production. Immunoaffinity columns prepared by entrapping anti-DON antibodies by the sol-gel method were used for sample clean-up. DON was quantified by high performance liquid chromatography (HPLC) and ultraviolet (UV) detection. In general, the incidence of DON contamination was rather low. In eight samples (14.5%) the DON concentration was above the LOQ (380ng/g), in six samples (10.9%) DON was detected but could not be quantified (>LOD (200ng/g), LOQ. The data indicate both a higher incidence of DON contamination and higher DON concentrations in food and feed samples from conventional than in those from organic production.

Keywords Pubmed: Animal Feed/analysis
Chromatography, Affinity/methods*
Chromatography, High Pressure Liquid/methods*
Food Analysis/methods*
Health Food/analysis
Immunosorbent Techniques*
Mycotoxins
Phase Transition
Spectrophotometry, Ultraviolet
Trichothecenes/analysis*
Triticum


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