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Gewählte Publikation:

Publikationstyp: Zeitschriftenaufsatz
Dokumentart: Originalarbeit

Publikationsjahr: 2011

AutorInnen: Alispahic, M; Christensen, H; Hess, C; Razzazi-Fazeli, E; Bisgaard, M; Hess, M

Titel: Identification of Gallibacterium species by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evaluated by multilocus sequence analysis.

Quelle: Int J Med Microbiol. 2011; 301(6):513-522



Autor/innen der Vetmeduni Vienna:

Alispahic Merima
Hess Claudia
Hess Michael
Razzazi-Fazeli Ebrahim

Beteiligte Vetmed-Organisationseinheiten
Klinische Abteilung für Geflügelmedizin
VetCore


Abstract:
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) whole-cell fingerprinting was used for characterization of 66 reference strains of Gallibacterium. The 4 recognised Gallibacterium species and Gallibacterium genomospecies 1 yielded reproducible and unique mass spectrum profiles, which were confirmed with Bruker Biotyper reference database version 3. The reproducibility of MALDI-TOF MS results were evaluated varying the age and storage of the cultures investigated. Reliable species identification was possible for up to 8 days of storage at 4°C and less reliable if the bacteria were stored at room temperature (20°C). However, if the strains were grown longer than 48h at 37°C under microaerobic atmosphere, poor identification results were obtained, due to changes in protein profile. The MALDI-TOF MS results of all 66 strains demonstrated 87.9% concordance with results based upon biochemical/physiological characterization. In addition, diversities outlined by MALDI-TOF MS were verified by sequencing the rpoB (n=43), 16S rRNA (n=28), infB (n=14), and recN (n=14) genes (multilocus sequence analysis, MLSA). In addition, discrepancies were observed between some of the genes sequenced. Results obtained demonstrated that MALDI-TOF MS fingerprinting represents a fast and reliable method for identification and differentiation of the 4 recognised Gallibacterium species and possible a fifth species Gallibacterium genomospecies 1, with applications in clinical diagnostics.

Keywords Pubmed: Bacterial Proteins/genetics
Bacterial Proteins/isolation & purification
DNA Restriction Enzymes/genetics
DNA Restriction Enzymes/isolation & purification
Databases, Genetic
Gene Library
Multilocus Sequence Typing/methods*
Pasteurellaceae/classification
Pasteurellaceae/isolation & purification*
Phenotype
Prokaryotic Initiation Factor-2/genetics
Prokaryotic Initiation Factor-2/isolation & purification
RNA, Ribosomal, 16S/genetics
RNA, Ribosomal, 16S/isolation & purification
Software
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods*


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