Veterinärmedizinische Universität Wien Forschungsinformationssystem VetDoc

Grafischer Link zur Startseite der Vetmeduni Vienna

Gewählte Publikation:

Publikationstyp: Zeitschriftenaufsatz
Dokumentart: Originalarbeit

Publikationsjahr: 2012

AutorInnen: Ametaj, BN; Sivaraman, S; Dunn, SM; Zebeli, Q

Titel: Repeated oral administration of lipopolysaccharide from Escherichia coli 0111:B4 modulated humoral immune responses in periparturient dairy cows.

Quelle: Innate Immun. 2012; 18(4):638-647



Autor/innen der Vetmeduni Vienna:

Zebeli Qendrim

Beteiligte Vetmed-Organisationseinheiten
Institut für Tierernährung und funktionelle Pflanzenstoffe


Abstract:
The objective of this study was to evaluate the effects of repeated oral exposure to LPS on humoral immune responses of periparturient dairy cows. Sixteen Holstein cows were assigned to two treatment groups 2 wk before the expected day of parturition. Cows were administered orally, twice weekly at wk -2, -1 and +1 around parturition, with the following treatments: 3 ml saline; or 3 ml of saline containing LPS from Escherichia coli 0111:B4. The amount of LPS administered during wk -2, -1, and +1 was 0.01, 0.05, or 0.1 µg/kg body weight, respectively. Multiple blood samples were collected by jugular vein and various immune and clinical variables were measured. Results indicated that, on one hand, concentrations of plasma IgG anti-LPS Abs decreased (P < 0.01) and those of IgM anti-LPS Abs increased (P < 0.01) in cows treated with oral LPS. On the other hand, there were no overall differences (P > 0.05) in the concentrations of serum amyloid A, LPS-binding protein, haptoglobin, cortisol, IgA anti-LPS Abs in the plasma, feed intake, body temperature and rumen contractions rate between the control and treatment groups. To our knowledge, this study is the first to show that repeated oral administration with LPS from E. coli 0111:B4 has the potential to stimulate humoral immune responses in periparturient dairy cows.

Keywords Pubmed: Administration, Oral
Animals
Cattle/immunology*
Female
Immunity, Humoral/drug effects*
Immunization/methods*
Immunoglobulin G/biosynthesis
Immunoglobulin G/blood
Immunoglobulin M/biosynthesis
Immunoglobulin M/blood
Lipopolysaccharides/administration & dosage*
Peripartum Period/immunology*


© Veterinärmedizinische Universität Wien Hilfe und Downloads