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Gewählte Publikation:

Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Kongressbeitrag Originalarbeit

Jahr: 1998

AutorInnen: Knasmüller, S; Parzefall, W; Sanyal, R; Ecker, S; Schwab, C; Uhl, M; Mersch-Sundermann, V; Williamson, G; Hietsch, G; Langer, T; Darroudi, F; Natarajan, AT

Titel: Use of metabolically competent human hepatoma cells for the detection of mutagens and antimutagens.

Quelle: 5th International Conference on Mechanisms of Antimutagenesis and Anticarcinogenesis (5th ICMAA), OKAYAMA, JAPAN, Japan, DEC 02-06, 1996. Mutat Res-Fund Mol M (402), 1-2 185-202.

Beteiligte Vetmed-Organisationseinheiten
Institut für Tierernährung und funktionelle Pflanzenstoffe

The human hepatoma line (Hep G2) has retained the activities of various phase I and phase II enzymes which play a crucial role in the activation/detoxification of genotoxic procarcinogens and reflect the metabolism of such compounds in vivo better than experimental models with metabolically incompetent cells and exogenous activation mixtures. In the last years, methodologies have been developed which enable the detection of genotoxic effects in Hep G2 cells. Appropriate endpoints are the induction of 6-TGr mutants, of micronuclei and of comets (single cell gel electrophoresis assay). It has been demonstrated that various classes of environmental carcinogens such as nitrosamines, aflatoxins, aromatic and heterocyclic amines and polycyclic aromatic hydrocarbons can be detected in genotoxicity assays with Hep G2 cells. Furthermore, it has been shown that these assays can distinguish between structurally related carcinogens and non-carcinogens, and positive results have been obtained with rodent carcinogens (such as safrole and hexamethylphosphoramide) which give false negative results in conventional in vitro assays with rat liver homogenates. Hep G2 cells have also been used in antimutagenicity studies and can identify mechanisms not detected in conventional in vitro systems such as induction of detoxifying enzymes, inactivation of endogenously formed DNA-reactive metabolites and intracellular inhibition of activating enzymes.

Keywords Pubmed: Animals
Antimutagenic Agents/pharmacology*
Carcinoma, Hepatocellular/enzymology
Carcinoma, Hepatocellular/metabolism*
Carcinoma, Hepatocellular/pathology
Liver Neoplasms/enzymology
Liver Neoplasms/metabolism*
Liver Neoplasms/pathology
Mutagenicity Tests
Tumor Cells, Cultured

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