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Gewählte Publikation:

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Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Originalarbeit

Jahr: 2020

AutorInnen: Ballas, P; Rückert, C; Wagener, K; Drillich, M; Kämpfer, P; Busse, HJ; Ehling-Schulz, M

Titel: Corynebacterium urogenitale sp. nov. isolated from the genital tract of a cow.

Quelle: Int J Syst Evol Microbiol. 2020; 70(6):3625-3632



Autor/innen der Vetmeduni Vienna:

Ballas Panagiotis
Busse Hans-Jürgen
Drillich Marc
Ehling-Schulz Monika
Wagener Karen

Beteiligte Vetmed-Organisationseinheiten
Institut für Mikrobiologie, Abteilung für Funktionelle Mikrobiologie
Universitätsklinik für Wiederkäuer, Bestandsbetreuung bei Wiederkäuern
Institut für Mikrobiologie


Zugehörige(s) Projekt(e): Deciphering the role of the opportunistic pathogen Bacillus pumilus and Streptococcus uberis in bovine postpartum uterine infections

Deciphering the role of the opportunistic pathogen Bacillus pumilus and Streptococcus uberis in bovine postpartum uterine infections


Abstract:
A Gram-stain-positive bacterial isolate, designated LMM-1652T, was isolated from an intrauterine cytobrush sample originating from a postpartum Holstein Friesian dairy cow. The strain had a rod to coccoid-shape, was catalase-positive and oxidase-negative. 16S rRNA gene sequence similarity analyses revealed that its closest relatives were Corynebacterium falsenii (97.05 % similarity), Corynebacterium jeikeium (96.83 %) and Corynebacterium urealyticum (96.82 %). Subsequent whole genome analysis showed that the genome-to-genome distance of strain LMM-1652T to its closest relatives was in the range of 23.2-24.8 %, while the average nucleotide identity values ranged from 73.7 to 74.3%, thus confirming that this isolate represents a novel species. Strain LMM-1652T was characterized by a quinone system mainly consisting of MK-9(H2) and MK-10(H2). The polar lipids profile of the strain consisted mainly of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol-mannoside, as well as one unidentified lipid lacking any functional group. Smaller amounts of four unidentified phospholipids, four unidentified glycolipids, β-gentiobiosyl diacylglycerol and four unidentified lipids lacking a functional group were also found. The cell wall contained meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan. The fatty acid profile was mainly composed of C18 : 1 ω9c, C18 : 0 and C16 : 0. We propose a novel species of the genus Corynebacterium with the name Corynebacterium urogenitale LMM-1652T (=LMG 31163T=DSM 108747T).

Keywords Pubmed: Animals
Bacterial Typing Techniques
Base Composition
Cattlemicrobiology
Corynebacteriumclassificationisolation & purification
DNA, Bacterialgenetics
Diaminopimelic Acidchemistry
Fatty Acidschemistry
Female
Peptidoglycanchemistry
Phospholipidschemistry
Phylogeny
RNA, Ribosomal, 16Sgenetics
Sequence Analysis, DNA
Slovakia
Uterusmicrobiology
Vitamin K 2analogs & derivativeschemistry

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