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Gewählte Publikation:

Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Originalarbeit

Jahr: 2006

AutorInnen: Grabensteiner, E; Liebhart, D; Weissenböck, H; Hess, M

Titel: Broad dissemination of Histomonas meleagridis determined by the detection of nucleic acid in different organs after experimental infection of turkeys and specified pathogen-free chickens using a mono-eukaryotic culture of the parasite.

Quelle: Parasitol Int. 2006; 55(4):317-322

Autor/innen der Vetmeduni Vienna:

Grabensteiner Elvira
Hess Michael
Liebhart Dieter
Weissenböck Herbert

Beteiligte Vetmed-Organisationseinheiten
Institut für Pathologie
Universitätsklinik für Geflügel und Fische

Histomonas meleagridis, a flagellated protozoan parasite, is the causative agent of histomonosis (syn. histomoniasis, blackhead) in turkeys and chickens. The organs primarily affected by the parasite are the caeca and the liver. Until now, only few reports exist in which the parasite has been diagnosed in tissues other than those mentioned above. Hence, the aim of this study was to perform a systematic investigation of various organs of turkeys and specified pathogen-free chickens following an experimental infection with a mono-eukaryotic culture of Histomonas meleagridis in order to determine the dissemination of the flagellate in infected birds. Molecular methods like PCR and in situ hybridization were used for this purpose. For the first time, the DNA of the parasite could be detected in 13 different organs of infected turkeys by PCR including the proventriculus, duodenum, jejunum, caeca, pancreas, bursa of Fabricius, liver, kidney, spleen, heart, lung, thymus and the brain. Most of these findings were further confirmed by in situ hybridization. In contrast to the turkeys that all died shortly after the infection, all of the chickens survived without displaying any clinical symptoms. Even at necropsy, only mild pathological changes were observed in the caeca. Nevertheless, the parasite could also be detected in various organs of these birds, namely the caeca, bursa of Fabricius, kidney, heart and the brain.

Keywords Pubmed: Animals
DNA, Protozoan/isolation & purification
In Situ Hybridization/methods
In Situ Hybridization/veterinary
Polymerase Chain Reaction/methods
Polymerase Chain Reaction/veterinary
Poultry Diseases/diagnosis
Poultry Diseases/parasitology*
Poultry Diseases/pathology
Protozoan Infections/diagnosis
Protozoan Infections/parasitology
Protozoan Infections/pathology
Protozoan Infections, Animal*
Sensitivity and Specificity
Specific Pathogen-Free Organisms
Trichomonadida/isolation & purification

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