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Gewählte Publikation:

Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Originalarbeit

Jahr: 2006

AutorInnen: Schäfer-Somi, S; Kluger, S; Knapp, E; Klein, D; Aurich, C

Titel: Effects of semen extender and semen processing on motility and viability of frozen-thawed dog spermatozoa.

Quelle: Theriogenology. 2006; 66(2):173-182



Autor/innen der Vetmeduni Vienna:

Aurich Christine
Klein Dieter
Kluger Silvia
Schäfer-Somi Sabine

Beteiligte Vetmed-Organisationseinheiten
Institut für Virologie
Plattform Besamung und Embryotransfer


Zugehörige(s) Projekt(e): Andrologie und Spermienfunktionsdiagnostik


Abstract:
The aims of the present study were, to assess the effects of semen centrifugation, two different diluents and two different freezing methods on post-thaw semen quality in canine semen, and to elucidate the interdependence of these parameters. For this purpose, the sperm-rich fractions of ejaculates from 12 healthy male beagles were divided into four aliquots. Two aliquots were centrifuged and resuspended with two TRIS-egg yolk based extenders: with Uppsala and Gill extender (Gill). The diluents differed in the concentration of glycerol and in the admixture of Equex STM paste (Nova Chemical Sales Inc., Scituate, MA, USA). Diluted semen was frozen either in a styrofoam box or with a computerized freezing machine and an optimized freezing curve (IceCube 1,810; Sy-Lab, Purkersdorf, A). The change in temperature inside the straws was measured during the freezing procedure. Thawed semen samples were assessed for motility and viability (SYBR-14/PI) using the computer assisted sperm analyzer SpermVision (Minitüb, G) and a modified triple staining technique (flow cytometry). Deep freezing in the machine resulted in better motility and viability than in the box. The combination centrifugation-Uppsala extender-machine was superior to all other combinations, which was most evident after storage at +5 degrees C for 7 h (motility: 53.1%, viability: 64.9%). Post-thaw longevity and progressive motility were significantly improved by the use of the here introduced freezing curve. This was shown to be partly caused by less pronounced fluctuations of temperature inside the straws when compared to box-freezing.

Keywords Pubmed: Animals
Cell Survival
Cryopreservation/instrumentation
Cryopreservation/methods
Cryopreservation/veterinary*
Cryoprotective Agents/pharmacology*
Dogs/physiology*
Flow Cytometry/veterinary
Male
Semen Preservation/instrumentation
Semen Preservation/methods
Semen Preservation/veterinary*
Specimen Handling/methods
Specimen Handling/veterinary
Sperm Count/veterinary
Sperm Motility
Spermatozoa/physiology*
Time Factors


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