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Gewählte Publikation:

Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Originalarbeit

Jahr: 2006

AutorInnen: Dunham, SP; Bruce, J; Klein, D; Flynn, JN; Golder, MC; MacDonald, S; Jarrett, O; Neil, JC

Titel: Prime-boost vaccination using DNA and whole inactivated virus vaccines provides limited protection against virulent feline immunodeficiency virus.

Quelle: Vaccine. 2006; 24(49-50):7095-7108



Autor/innen der Vetmeduni Vienna:

Klein Dieter

Beteiligte Vetmed-Organisationseinheiten
Institut für Virologie


Zugehörige(s) Projekt(e): Virus-Wirt Interaktionen


Abstract:
Protection against feline immunodeficiency virus (FIV) has been achieved using a variety of vaccines notably whole inactivated virus (WIV) and DNA. However protection against more virulent isolates, typical of those encountered in natural infections, has been difficult to achieve. In an attempt to improve protection against virulent FIV(GL8), we combined both DNA and WIV vaccines in a "prime-boost" approach. Thirty cats were divided into four groups receiving vaccinations and one unvaccinated control group. Following viral challenge, two vaccinated animals, one receiving DNA alone and one the prime-boost vaccine remained free of viraemia, whilst all controls became viraemic. Animals vaccinated with WIV showed apparent early enhancement of infection at 2 weeks post challenge (pc) with higher plasma viral RNA loads than control animals or cats immunised with DNA alone. Despite this, animals vaccinated with WIV or DNA alone showed significantly lower proviral loads in peripheral blood mononuclear cells and mesenteric lymph node cells, whilst those receiving the DNA-WIV prime-boost vaccine showed significantly lower proviral loads in PBMC, than control animals, at 35 weeks pc. Therefore both DNA and WIV vaccines conferred limited protection against viral challenge but the combination of WIV and DNA in a prime-boost approach appeared to offer no significant advantage over either vaccine alone.

Keywords Pubmed: Animals
Antibodies, Viral/analysis
Antibodies, Viral/biosynthesis
Cats
Enzyme-Linked Immunosorbent Assay
Feline Acquired Immunodeficiency Syndrome/immunology*
Feline Acquired Immunodeficiency Syndrome/prevention & control*
Immunity, Cellular/immunology
Immunization Schedule
Immunization, Secondary
Immunodeficiency Virus, Feline/immunology*
Integrases/genetics
Integrases/immunology
Interferon-gamma/metabolism
Lymph Nodes/virology
Lymphocyte Subsets/immunology
Lymphocyte Subsets/virology
RNA, Viral/blood
Spleen/virology
Vaccines, DNA/immunology
Vaccines, Inactivated/immunology
Viral Load
Viral Vaccines/therapeutic use*
Viremia/immunology
Viremia/virology


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