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Gewählte Publikation:

Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Übersichtsarbeit

Jahr: 2002

AutorInnen: Klein, D

Titel: Quantification using real-time PCR technology: applications and limitations.

Quelle: Trends Mol Med. 2002; 8(6):257-260



Autor/innen der Vetmeduni Vienna:

Klein Dieter

Beteiligte Vetmed-Organisationseinheiten
Institut für Virologie


Zugehörige(s) Projekt(e): Virus-Wirt Interaktionen


Abstract:
The introduction of real-time PCR technology has significantly improved and simplified the quantification of nucleic acids, and this technology has become an invaluable tool for many scientists working in different disciplines. Especially in the field of molecular diagnostics, real-time PCR-based assays have gained favour in the recent past. However, the wide use of real-time PCR methods has also highlighted some of the critical points and limitations of these assays. These aspects must be considered to increase the reliability of the obtained data.

Keywords Pubmed: Gene Expression Profiling
Humans
Medical Oncology/methods
Microbiological Techniques
Molecular Diagnostic Techniques/methods*
Polymerase Chain Reaction/methods*
Time Factors


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