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Gewählte Publikation:

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Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Originalarbeit

Jahr: 2002

AutorInnen: Oztürk-Winder, F; Renner, M; Klein, D; Müller, M; Salmons, B; Günzburg, WH

Titel: The murine whey acidic protein promoter directs expression to human mammary tumors after retroviral transduction.

Quelle: Cancer Gene Ther (9), 5 421-431.



Autor/innen der Vetmeduni Vienna:

Günzburg Walter
Klein Dieter
Müller Mathias
Renner Matthias

Beteiligte Vetmed-Organisationseinheiten
Institut für Tierzucht und Genetik
Institut für Virologie
Interuniversitäres Department für Agrarbiotechnologie (IFA)


Zugehörige(s) Projekt(e): Genomregulation und Gentherapie in Human- und Veterinärmedizin


Abstract:
The whey acidic protein (WAP) promoter is known to be active in pregnant and lactating mammary epithelial cells as well as mammary tumors of mice. Here we show that a proximal fragment of the murine WAP promoter, including most elements postulated as being responsible for mammary-specific regulation, confers mammary-specific expression upon a marker gene in transgenic mice even though the distal promoter region, known to be important for rat WAP promoter activity, is lacking. The relatively small size of this fragment allows its insertion into a murine leukemia virus-based retroviral vector in place of the viral promoter. Infection of a number of established human mammary and nonmammary cell lines with such a retroviral vector revealed that the WAP promoter was limited in its activity to mammary tumor cell lines. Expression in tumorigenic mammary cells was even more pronounced when these cells were introduced into the mammary fat pads of mice. This is the first demonstration that the WAP promoter is active in human mammary cells and mammary tumor cells in general, and suggests that the extended proximal WAP promoter may be useful for directing therapeutic gene expression to human mammary tumors.

Keywords Pubmed: 3T3 Cells
Animals
Blotting, Northern
Blotting, Southern
Breast Neoplasms/metabolism*
Breast Neoplasms/therapy*
Collagen/metabolism
Humans
Mice
Mice, SCID
Mice, Transgenic
Milk Proteins/genetics*
Models, Genetic
Promoter Regions, Genetic*
Rats
Retroviridae/genetics
Reverse Transcriptase Polymerase Chain Reaction
Transduction, Genetic
Transfection
beta-Galactosidase/metabolism


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