Veterinärmedizinische Universität Wien Forschungsinformationssystem VetDoc

Grafischer Link zur Startseite der Vetmeduni Vienna

Gewählte Publikation:

Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Originalarbeit

Jahr: 2008

AutorInnen: Wolfesberger, B; Tonar, Z; Witter, K; Guija de Arespacohaga, A; Skalicky, M; Walter, I; Thalhammer, JG; Egger, GF

Titel: Microvessel density in normal lymph nodes and lymphomas of dogs and their correlation with vascular endothelial growth factor expression.

Quelle: Res Vet Sci. 2008; 85(1):56-61

Autor/innen der Vetmeduni Vienna:

Guija-De-Arespacochaga Abigail
Skalicky Monika
Thalhammer Johann
Walter Ingrid
Witter Kirsti
Wolfesberger Birgitt

Beteiligte Vetmed-Organisationseinheiten
Institut für Physiologie, Pathophysiologie und Biophysik, Abteilung für Physiologie, Pathophysiologie und experimentelle Endokrinologie
Institut für Topographische Anatomie
Universitätsklinik für Kleintiere, Klinische Abteilung für Interne Medizin Kleintiere
Plattform Labordiagnostik

Microvessel density is a frequently used parameter of angiogenesis, which is a complex multistep process necessary for tumor progression. The aim of this study was to compare the microvessel density of normal lymph node biopsies with those diagnosed with lymphoma in dogs. Furthermore, we sought to determine if there was any correlation between microvessel density and vascular endothelial growth factor expression in canine lymphoma, representing a potential target for anti-angiogenic therapy. Combined immunohistochemistry (von Willebrand factor) and lectin histochemistry was used to highlight microvessels in 40 untreated canine lymphomas and 14 normal lymph nodes. To evaluate microvessel density, the number of profiles of blood vessels per unit area was calculated. Fifty image fields (a total area of 5.68 mm(2)) were sampled for each specimen in a systematic random, way. We found a significant difference between the microvessel densities (MVD) of normal and neoplastic lymph nodes (177+/-35 versus 241+/-72 microvessel profiles/mm(2)). Classifying lymphoma samples according to the working formulation and the Kiel classification system revealed no significant differences in MVD between different grade malignancies. Immunohistochemical demonstration of the proangiogenic protein vascular endothelial growth factor showed expression in 60% of canine lymphomas, although there was no correlation between microvessel density and vascular endothelial growth factor expression. As an increase in tumor angiogenesis was observed in lymphoma samples compared to normal canine lymph node tissue, additional anti-angiogenic therapy, besides conventional chemotherapy as a lymphoma treatment may be effective. The optimal target among many pro-angiogenic factors has yet to be elucidated.

Keywords Pubmed: Animals
Antineoplastic Agents/therapeutic use
Dog Diseases/pathology*
Gene Expression Regulation/physiology
Lymph Nodes/anatomy & histology
Lymph Nodes/blood supply*
Lymphoma/drug therapy
Vascular Endothelial Growth Factor A/genetics
Vascular Endothelial Growth Factor A/metabolism*

© Veterinärmedizinische Universität Wien Hilfe und DownloadsErklärung zur Barrierefreiheit