To assess genetic variability of brown hares (Lepus europaeus PALLAS. 1778) from the Vojvodina province (Serbia) and to reveal gene pool relationships with central and southeastern European populations, 33 hares were screened for allozymic variation Lit 40 putative structural gene loci and directly compared to earlier data sets of 20 Austrian and eight Bulgarian Populations. Seven loci (17.5% rate of polymorphism) were polymorphic in the Vojvodinian hares. All indices of genetic diversity of the Vojvodina Population were within the ranges, found for the Austrian and Bulgarian Populations, indicating no loss of genetic diversity. Pairwise genetic distances revealed low levels of divergence among all compared Populations. Low bootstrap Values I)provided little Support for allocating the Vojvodina hares to either the central European or southeastern European Populations. Multidimensional scaling (MDS) of the pairwise Rogers' distance matrix and multivariate analysis of variance of population coordinates for three dimensions revealed significant differentiation between central and south- eastern European populations. Stepwise discriminant analysis of population coordinates from MDS classified the Vojvodina population with 69.0% into the group of central European populations.