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Gewählte Publikation:

Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Originalarbeit

Jahr: 2009

AutorInnen: Klonjkowski, B; Klein, D; Galea, S; Gavard, F; Monteil, M; Duarte, L; Fournier, A; Sayon, S; Górna, K; Ertl, R; Cordonnier, N; Sonigo, P; Eloit, M; Richardson, J

Titel: Gag-specific immune enhancement of lentiviral infection after vaccination with an adenoviral vector in an animal model of AIDS.

Quelle: Vaccine. 2009; 27(6):928-939



Autor/innen der Vetmeduni Vienna:

Ertl Reinhard
Klein Dieter

Beteiligte Vetmed-Organisationseinheiten
Institut für Virologie


Abstract:
The evaluation of vaccine strategies in animal models is essential for the development of a vaccine against HIV. In efficacy trials conducted in non-human primate models of AIDS, vaccines based on adenoviruses compared favourably with other vaccine vectors. To determine whether this strategy could be transposed to another animal model, and by extension, to humans, we have evaluated the efficacy of adenoviral vectors in a natural model of AIDS, infection of the cat by the feline immunodeficiency virus (FIV). Recombinant canine adenoviruses expressing the envelope glycoproteins or the Gag protein of a primary strain of FIV were constructed. Three groups of six cats were immunised twice with vectors expressing FIV antigens or with a vector expressing an irrelevant antigen, green fluorescent protein, by intramuscular and subcutaneous routes. Humoral responses were elicited against the transgene product in 6/6, 3/6 and 0/6 cats after immunisation against green fluorescent protein, Gag or the envelope glycoproteins, respectively. Six weeks after the second administration, cats were challenged by the intraperitoneal route with the homologous strain, and viral burden in plasma was followed by quantitative RT-PCR. Immunisation with FIV antigens did not afford protection. Rather, viral RNA was detected at earlier time points in cats immunised against Gag than in cats immunised with a vector expressing an irrelevant antigen. Such immune-mediated enhancement did not appear to have a long-range impact on viral set point or inversion of the CD4(+)/CD8(+) ratio. Thus, in the feline AIDS model pre-existing immunity against a viral antigen exacerbated acute phase infection.

Keywords Pubmed: AIDS Vaccines/genetics
AIDS Vaccines/immunology*
Adenoviridae/genetics*
Animals
Antibodies, Viral/blood
CD4-CD8 Ratio
Cats
Enzyme-Linked Immunosorbent Assay
Feline Acquired Immunodeficiency Syndrome/prevention & control
Female
Gene Products, env/genetics
Gene Products, env/immunology
Gene Products, gag/genetics
Gene Products, gag/immunology*
Immunization, Secondary
Immunodeficiency Virus, Feline/genetics
Immunodeficiency Virus, Feline/immunology*
Male
Mice
Mice, Inbred BALB C
RNA, Viral/blood
Reverse Transcriptase Polymerase Chain Reaction
Viral Load


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