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Gewählte Publikation:

Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Originalarbeit

Jahr: 2009

AutorInnen: Goüy de Bellocq, J; Suchentrunk, F; Baird, SJ; Schaschl, H

Titel: Evolutionary history of an MHC gene in two leporid species: characterisation of Mhc-DQA in the European brown hare and comparison with the European rabbit.

Quelle: Immunogenetics. 2009; 61(2):131-144



Autor/innen der Vetmeduni Vienna:

Suchentrunk Franz

Beteiligte Vetmed-Organisationseinheiten
Forschungsinstitut für Wildtierkunde und Ökologie


Abstract:
We surveyed the genetic diversity of the expressed major histocompatibility complex class II DQA locus in natural populations of European brown hares, Lepus europaeus, from Austria and Belgium (267 individuals in total). Based on cDNA sequences, we designed hare-specific primers to amplify the highly variable second exon of the DQA gene. Using cloning-sequencing methodology and capillary electrophoresis single-strand conformation polymorphism, we found ten alleles of the DQA exon 2 locus across these two European regions, of which eight are described for the first time. To search for signals of selection and recombination in the evolution of the DQA gene within the leporids, we augmented our sample with orthologous DQA alleles from the European rabbit, Oryctolagus cuniculus, in order to carry out a species level, species pairwise comparison. We found evidence of recombination in the history of the DQA sequences in leporids with some recombinant alleles bridging the species divide. In both species, selection on peptide binding site codons can be detected, though stronger for the rabbit. This result suggests that there may be a differential selection pressure in the deeper evolutionary history of these two species due to differences in several demographic and ecological traits likely subjecting them to differential selection by parasites. Finally, evolutionary relationships show a widespread and statistically significant intermingling of alleles from the two species. The many macroparasites shared between hares and rabbits may explain this pattern of trans-species polymorphism.

Keywords Pubmed: Alleles
Amino Acid Sequence
Amino Acid Substitution
Animals
Animals, Laboratory/genetics
Animals, Wild/genetics
Austria
Belgium
Codon/genetics
Conserved Sequence
Evolution, Molecular*
Gene Frequency
Genes, MHC Class II*
Genetic Speciation*
Genotype
Hares/genetics*
Molecular Sequence Data
Phylogeny
Polymorphism, Genetic
Rabbits/genetics*
Recombination, Genetic
Selection, Genetic
Sequence Alignment
Sequence Homology, Amino Acid
Species Specificity
Transcription, Genetic


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