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Gewählte Publikation:

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Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Originalarbeit

Jahr: 2009

AutorInnen: Paar, M; Klein, D; Salmons, B; Günzburg, WH; Renner, M; Portsmouth, D

Titel: Influence of vector design and host cell on the mechanism of recombination and emergence of mutant subpopulations of replicating retroviral vectors.

Quelle: BMC Mol Biol. 2009; 10:8



Autor/innen der Vetmeduni Vienna:

Günzburg Walter
Klein Dieter
Portsmouth Daniel
Renner Matthias

Beteiligte Vetmed-Organisationseinheiten
Institut für Virologie
VetCore


Abstract:
The recent advent of murine leukaemia virus (MLV)-based replication-competent retroviral (RCR) vector technology has provided exciting new tools for gene delivery, albeit the advances in vector efficiency which have been realized are also accompanied by a set of fresh challenges. The expression of additional transgene sequences, for example, increases the length of the viral genome, which can lead to reductions in replication efficiency and in turn to vector genome instability. This necessitates efforts to analyse the rate and mechanism of recombinant emergence during the replication of such vectors to provide data which should contribute to improvements in RCR vector design.

Keywords Pubmed: Animals
Base Sequence
Cell Line
Cell Line, Tumor
Genetic Vectors/genetics*
Genetic Vectors/metabolism
Humans
Male
Mice
Mice, Nude
Molecular Sequence Data
NIH 3T3 Cells
Recombination, Genetic/genetics*
Retroviridae/genetics*
Retroviridae/metabolism
Sequence Deletion/genetics*
Virus Replication/genetics*


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