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Gewählte Publikation:

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Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Originalarbeit

Jahr: 1997

AutorInnen: Klein, D; Indraccolo, S; von Rombs, K; Amadori, A; Salmons, B; Günzburg, WH

Titel: Rapid identification of viable retrovirus-transduced cells using the green fluorescent protein as a marker.

Quelle: Gene Ther. 1997; 4(11):125-160



Autor/innen der Vetmeduni Vienna:

Günzburg Walter
Klein Dieter

Beteiligte Vetmed-Organisationseinheiten
Institut für Virologie


Zugehörige(s) Projekt(e): Genomregulation und Gentherapie in Human- und Veterinärmedizin


Abstract:
Various methods for determining the expression of the beta-galactosidase (beta-gal) gene after retroviral transduction were compared as a means to assess retroviral titre. To allow better comparison, different retroviral vectors were constructed carrying two mutants of the green fluorescent protein and assessed as sensitive markers of retroviral gene transfer. It could be demonstrated that GFP is generally superior to beta-gal in terms of sensitivity, speed and non-invasiveness of assay, allowing easy direct FACS sorting of populations of transduced cells. This opens the possibility of enrichment by sorting of ex vivo transduced cells in gene therapy protocols.

Keywords Pubmed: Animals
Cell Line
Cell Separation
Flow Cytometry
Gene Transfer Techniques*
Genetic Markers*
Genetic Vectors*
Green Fluorescent Proteins
Humans
Luminescent Measurements
Luminescent Proteins*/genetics*
Retroviridae*
beta-Galactosidase/genetics*


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