Veterinärmedizinische Universität Wien Forschungsinformationssystem VetDoc
Gewählte Publikation:
Publikationstyp:
Zeitschriftenaufsatz
Dokumenttyp:
Originalarbeit
Jahr:
2010
AutorInnen:
Wolfesberger, B; Tonar, Z; Gerner, W; Skalicky, M; Heiduschka, G; Egerbacher, M; Thalhammer, JG; Walter, I
Titel:
The tyrosine kinase inhibitor sorafenib decreases cell number and induces apoptosis in a canine osteosarcoma cell line.
Quelle:
Res Vet Sci. 2010; 88(1):94-100
Autor/innen der Vetmeduni Vienna:
- Institut für Physiologie, Pathophysiologie und Biophysik, Abteilung für Physiologie, Pathophysiologie und experimentelle Endokrinologie
- Institut für Immunologie
- Institut für Topographische Anatomie
- Universitätsklinik für Kleintiere, Klinische Abteilung für Interne Medizin Kleintiere
Beteiligte Vetmed-Organisationseinheiten
- Canine osteosarcoma, an aggressive cancer with early distant metastasis, shows still despite good chemotherapy protocols poor long term survival. The aim of our study was to determine whether sorafenib, a novel multikinase inhibitor, has any effect on D-17 canine osteosarcoma cells. A cell proliferation kit was used for detecting surviving cells after treatment for 72 h with sorafenib or carboplatin or their combination. A significant decrease of neoplastic cells was observed after incubation with 0.5-16 microM sorafenib or with 80-640 microM carboplatin. Using immunocytochemistry for activated caspase 3 to evaluate apoptosis, we found significantly more positive cells in the sorafenib treated groups. Paradoxically, expression of the nuclear proliferation marker Ki-67 was also significantly higher in sorafenib treated cells. The drug sorafenib showed potent antitumour activity against D-17 canine osteosarcoma cells in vitro, suggesting a potential as a therapeutic tool in the treatment of bone cancer in dogs.
Abstract:
Keywords Pubmed: Animals
Antineoplastic Agents/pharmacology
Antineoplastic Agents/therapeutic use*
Apoptosis/drug effects*
Benzenesulfonates/pharmacology
Benzenesulfonates/therapeutic use*
Bone Neoplasms/drug therapy
Bone Neoplasms/enzymology
Bone Neoplasms/veterinary*
Caspase 3/metabolism
Cell Count
Cell Line, Tumor
Dog Diseases/drug therapy*
Dog Diseases/enzymology
Dogs
Flow Cytometry
Ki-67 Antigen/metabolism
Microscopy, Electron, Transmission
Niacinamide/analogs & derivatives
Osteosarcoma/drug therapy
Osteosarcoma/enzymology
Osteosarcoma/veterinary*
Phenylurea Compounds
Protein-Tyrosine Kinases/antagonists & inhibitors*
Pyridines/pharmacology
Pyridines/therapeutic use*