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Gewählte Publikation:

Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Originalarbeit

Jahr: 1998

AutorInnen: Mrochen, S; Klein, D; Nikol, S; Smith, JR; Salmons, B; Günzburg, WH

Titel: Inducible expression of p21WAF-1/CIP-1/SDI-1 from a promoter conversion retroviral vector.

Quelle: J Mol Med (75), 11-12 820-828.



Autor/innen der Vetmeduni Vienna:

Günzburg Walter
Klein Dieter

Beteiligte Vetmed-Organisationseinheiten
Institut für Virologie


Zugehörige(s) Projekt(e): Genomregulation und Gentherapie in Human- und Veterinärmedizin


Abstract:
Constitutive, high-level expression of the potentially therapeutic WAF-1/CIP-1/SDI-1 gene is incompatible with cell growth. A promoter conversion retroviral vector carrying the WAF-1/CIP-1/SDI-1 gene under the transcriptional control of the glucocorticoid inducible promoter of mouse mammary tumor virus was used to infect human bladder carcinoma or feline kidney cells. Reduced cell growth due to a greater proportion of cells being in the G0/G1 phase of the cell cycle was observed when WAF-1/CIP-1/SDI-1 expression was activated by addition of glucocorticoid hormone. This system demonstrates the potential long-term therapeutic use of WAF-1/CIP-1/SDI-1 delivered by retroviral vectors for inhibiting the growth of rapidly proliferating cells. Moreover, the conditional expression of genes such as WAF-1/CIP-1/SDI-1 from such retroviral vectors may facilitate analysis of their function.

Keywords Pubmed: Animals
Cats
Cell Division/genetics
Cell Line
Cyclin-Dependent Kinase Inhibitor p21
Cyclins/biosynthesis
Cyclins/genetics*
Dexamethasone/pharmacology
Gene Expression Regulation/drug effects
Genetic Vectors/chemical synthesis
Genetic Vectors/genetics*
Genetic Vectors/metabolism
Humans
Leukemia Virus, Murine/genetics*
Mammary Tumor Virus, Mouse/genetics*
Mice
Promoter Regions, Genetic*
Tumor Cells, Cultured


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