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Gewählte Publikation:

Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Originalarbeit

Jahr: 2000

AutorInnen: Uckert, W; Becker, C; Gladow, M; Klein, D; Kammertoens, T; Pedersen, L; Blankenstein, T

Titel: Efficient gene transfer into primary human CD8+ T lymphocytes by MuLV-10A1 retrovirus pseudotype.

Quelle: Hum Gene Ther. 2000; 11(7):1005-1014



Autor/innen der Vetmeduni Vienna:

Klein Dieter

Beteiligte Vetmed-Organisationseinheiten
Institut für Virologie


Zugehörige(s) Projekt(e): Genomregulation und Gentherapie in Human- und Veterinärmedizin


Abstract:
Efficient and stable gene transfer into primary human T lymphocytes would greatly improve their use for adoptive transfer to treat acquired disorders, viral diseases, and cancer. We have constructed retroviral vector pseudotypes of amphotropic murine leukemia viruses (A-MuLV, MuLV-10A1), gibbon ape leukemia virus (GaLV), and feline endogenous virus (RD114) containing the enhanced green fluorescent protein (GFP) as a marker gene. Transduction of primary human CD8+ T lymphocytes by the different GFP-retrovirus pseudotypes revealed the superiority of MuLV-10A1 in comparison with A-MuLV, GaLV, and RD114, respectively. The superior transduction efficacy of CD8+ T cells by MuLV-10A1 correlates with a longer half-life of this pseudotype in comparison with A-MuLV and, as shown by interference analysis with the human T cell line HUT78, by the utilization of both the A-MuLV receptor (Pit2) and the GaLV receptor (Pit1) for cell entry.

Keywords Pubmed: CD8-Positive T-Lymphocytes/physiology*
CD8-Positive T-Lymphocytes/virology
Gene Transfer Techniques*
Genetic Vectors*
Green Fluorescent Proteins
Humans
Leukemia Virus, Murine/genetics*
Luminescent Proteins/genetics
Luminescent Proteins/metabolism
Receptors, Virus/genetics
Receptors, Virus/metabolism
Retroviridae/genetics
Transduction, Genetic


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