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Gewählte Publikation:

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Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Originalarbeit

Jahr: 2004

AutorInnen: Nitsch, R; Pohl, EE; Smorodchenko, A; Infante-Duarte, C; Aktas, O; Zipp, F

Titel: Direct impact of T cells on neurons revealed by two-photon microscopy in living brain tissue.

Quelle: J Neurosci. 2004; 24(10):2458-2464



Autor/innen der Vetmeduni Vienna:

Pohl Elena
Smorodchenko Alina

Diese Publikation wurde nicht im Namen der Vetmeduni Vienna erstellt und ist deshalb ausschließlich der persönlichen Publikationsliste des/der Autors/Autorin zugeordnet!


Abstract:
Encephalitogenic T cells invade the brain during neuroinflammation such as multiple sclerosis (MS), inducing damage to myelin sheaths and oligodendrocytes. Only recently, neuronal structures were reported to be a crucial target in the disease. Here, two-photon microscopy using ion-sensitive dyes revealed that within the complex cellular network of living brain tissue, proteolipid protein (PLP)-specific T cells and T cells recognizing the nonmurine antigen ovalbumin (OVA) directly and independently of the major histocompatibility complex (MHC) contact neurons in which they induce calcium oscillations. T cell contact finally resulted in a lethal increase in neuronal calcium levels. This could be prevented by blocking both perforin and glutamate receptors. For the first time, our data provide direct insight into the activity of T cells in the living brain and their detrimental impact on neurons.

Keywords Pubmed: Animals
Brain/cytology
Brain/metabolism
Brain/physiology*
Calcium Signaling/drug effects
Calcium Signaling/physiology
Cell Communication/immunology
Cell Communication/physiology
Enzyme Inhibitors/pharmacology
Fluorescent Dyes
Macrolides/pharmacology
Membrane Glycoproteins/antagonists & inhibitors
Mice
Mice, Inbred Strains
Myelin Proteolipid Protein/immunology
Neurons/cytology
Neurons/drug effects
Neurons/physiology*
Ovalbumin/immunology
Perforin
Pore Forming Cytotoxic Proteins
T-Lymphocytes/cytology
T-Lymphocytes/drug effects
T-Lymphocytes/physiology*


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