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Gewählte Publikation:

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Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Originalarbeit

Jahr: 2010

AutorInnen: Johne, R; Plenge-Bönig, A; Hess, M; Ulrich, RG; Reetz, J; Schielke, A

Titel: Detection of a novel hepatitis E-like virus in faeces of wild rats using a nested broad-spectrum RT-PCR.

Quelle: J Gen Virol. 2010; 91(Pt 3):750-758



Autor/innen der Vetmeduni Vienna:

Hess Michael

Beteiligte Vetmed-Organisationseinheiten
Universitätsklinik für Geflügel und Fische


Abstract:
Hepatitis E is a rare human disease in developed countries. It is caused by hepatitis E virus (HEV), which is probably transmitted zoonotically to humans from domestic pigs and wild boars. Multiple reports on the detection of HEV-specific antibodies in rats have suggested the presence of an HEV-related agent; however, infectious virus or a viral genome has not been demonstrated so far. Here, a nested broad-spectrum RT-PCR protocol was developed capable of detecting different HEV types including those derived from wild boar and chicken. Screening of 30 faecal samples from wild Norway rats (Rattus norvegicus) from Hamburg (Germany) resulted in the detection of two sequences with similarities to human, mammalian and avian HEV. Virus particles with a morphology reminiscent of HEV were demonstrated by immunoelectron microscopy in one of these samples and the virus was tentatively designated rat HEV. Genome fragments with sizes of 4019 and 1545 nt were amplified from two samples. Sequence comparison with human and avian strains revealed only 59.9 and 49.9 % sequence identity, respectively. Similarly, the deduced amino acid sequence for the complete capsid protein had 56.2 and 42.9 % identity with human and avian strains, respectively. Inoculation of the samples onto three different permanent rat liver cell lines did not result in detectable virus replication as assayed by RT-PCR with cells of the fifth virus passage. Further investigations are necessary to clarify the zoonotic potential of rat HEV and to assess its suitability to serve in a laboratory rat animal model for human hepatitis E.

Keywords Pubmed: Animals
Animals, Wild/virology*
Cell Line
Chickens
Cluster Analysis
Feces/virology*
Germany
Hepatitis E virus/genetics
Hepatitis E virus/isolation & purification*
Humans
Microscopy, Immunoelectron
Molecular Sequence Data
Phylogeny
RNA, Viral/genetics
RNA, Viral/isolation & purification*
Rats
Reverse Transcriptase Polymerase Chain Reaction/methods*
Sequence Analysis, DNA
Sequence Homology
Virion/ultrastructure


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