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Gewählte Publikation:

Publikationstyp: Zeitschriftenaufsatz
Dokumentart: Originalarbeit

Publikationsjahr: 2010

AutorInnen: Fischer, C; Drillich, M; Odau, S; Heuwieser, W; Einspanier, R; Gabler, C

Titel: Selected pro-inflammatory factor transcripts in bovine endometrial epithelial cells are regulated during the oestrous cycle and elevated in case of subclinical or clinical endometritis.

Quelle: Reprod Fertil Dev. 2010; 22(5):818-829



Autor/innen der Vetmeduni Vienna:

Drillich Marc

Diese Publikation wurde nicht im Namen der Vetmeduni Vienna erstellt und ist deshalb ausschließlich der persönlichen Publikationsliste des/der Autors/Autorin zugeordnet!


Abstract:
Endometrial cells take part in embryo-maternal communication, as well as supporting the immune system in defending against invading pathogens. The aim of the present study was to examine the mRNA expression of factors that have been suggested to be involved in both events in the bovine endometrial epithelium, namely bovine granulocyte chemotactic protein 2 (CXCL5), interleukin-1 beta (IL1B), IL6, IL8, tumour necrosis factor (TNF), cyclooxygenase 2 (PTGS2) and haptoglobin (HP). Samples were collected in vivo from cows on Days 21-27 postpartum by the cytobrush method to evaluate the correlation between inflammatory factors and uterine health (cows with signs of clinical or subclinical endometritis and healthy cows). Bovine uteri were collected at the abattoir to investigate oestrous cycle-dependent mRNA expression patterns. Real-time reverse transcription-polymerase chain reaction revealed that the expression of CXCL5, IL1B, IL8 and TNF mRNA was significantly higher in cows with subclinical or clinical endometritis compared with healthy cows. The expression of CXCL5, IL1B and IL8 mRNA was increased around ovulation compared with the luteal phase. There was no indication of either oestrous cycle-dependent expression or a correlation with uterine health for IL6, PTGS2 and HP transcripts. These results suggest that CXCL5, IL1B, IL8 and TNF may represent potential marker genes for the detection of cows with subclinical endometritis and for monitoring new therapeutic approaches.

Keywords Pubmed: Animals
Cattle
Chemokine CXCL5/genetics
Cyclooxygenase 2/genetics
Endometritis/metabolism*
Endometrium/chemistry*
Epithelial Cells/chemistry
Estrous Cycle/metabolism*
Female
Gene Expression*
Haptoglobins/genetics
Interleukin-1beta/genetics
Interleukin-6/genetics
Interleukin-8/genetics
RNA, Messenger/analysis*
Reverse Transcriptase Polymerase Chain Reaction
Specimen Handling/methods
Specimen Handling/veterinary
Tumor Necrosis Factor-alpha/genetics


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