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Gewählte Publikation:

Publikationstyp: Originalbeitrag in Sammelwerk
Dokumenttyp:

Jahr: 2011

AutorInnen: Razzazi, E; Reiter, EV

Titel: Sample preparation and clean up in mycotoxin analysis: principles, applications and recent developments.

Quelle: IN: De Saeger, S [Hrsg.]: Determining mycotoxins and mycotoxigenic fungi in food and feed. Cambridge, Woodhead, pp. 37-70. ISBN: 978-1-8456-9674-0.

Autor/innen der Vetmeduni Vienna:

Razzazi-Fazeli Ebrahim
Reiter Elisabeth

Beteiligte Vetmed-Organisationseinheiten
Institut für Tierernährung und funktionelle Pflanzenstoffe


Abstract:
Risk assessment and monitoring of mycotoxins in agricultural commodities is associated with an increasing demand for appropriate analytical methods. Owing to the introduction of regulatory limits for various mycotoxins, the determination of mycotoxins in food and feed has become an important research issue in routine and governmental laboratories. The most prevalent methods for qualitative and quantitative determination, with the exception of enzyme linked immunosorbent assay ( ELISA), are not able to analyse mycotoxins in complex matrices without sample pretreatment. In the same way as for all other analytes, sample preparation is one of the most crucial and important steps in the whole chain of analysis. There are different strategies used to determine mycotoxins in agricultural commodities as well as in biological samples, for example animal tissues, urine and so on. There are various approaches, such as solid phase extraction and liquid-liquid extraction; however, the most important and popular sample preparation technique used in mycotoxin analysis is immunoaffinity chromatography. In recent years there have been efforts to introduce selective and cost-effective strategies in sample pretreatment of mycotoxins. This chapter describes the significance of sample clean up in the analysis of major and abundant mycotoxins. Various conventional methods that are applied to sample preparation of mycotoxins in food, feed and biological samples will be discussed. Recent developments are included and different strategies for achieving more efficient extraction and separation are compared and presented.


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