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Gewählte Publikation:

Publikationstyp: Zeitschriftenaufsatz
Dokumentart: Originalarbeit

Publikationsjahr: 2009

AutorInnen: Gabler, C; Drillich, M; Fischer, C; Holder, C; Heuwieser, W; Einspanier, R

Titel: Endometrial expression of selected transcripts involved in prostaglandin synthesis in cows with endometritis.

Quelle: Theriogenology. 2009; 71(6):993-1004



Autor/innen der Vetmeduni Vienna:

Drillich Marc

Diese Publikation wurde nicht im Namen der Vetmeduni Vienna erstellt und ist deshalb ausschließlich der persönlichen Publikationsliste des/der Autors/Autorin zugeordnet!


Abstract:
Several cytokines and prostaglandins play an important role in preparing the endometrium for implantation and mediating pro-inflammatory events. The aim of the present study was to examine mRNA expression of interleukin 1alpha (IL-1alpha), interleukin receptor antagonist (IL-1-RN), cytosolic prostaglandin E synthase (cPGES), microsomal PGES (mPGES-1 and mPGES-2) and lipocalin-type PGDS (L-PGDS) in the bovine endometrium. Endometrial epithelium samples were collected ex vivo from cows with different status of health at day 21-27 postpartum on a dairy farm. Three groups (n=9 animals each) were defined: (1) healthy cows with no signs of endometritis (control group), (2) cows with subclinical endometritis, and (3) cows with signs of clinical endometritis. Oestrous cycle-dependent mRNA expression pattern was investigated using bovine endometrial epithelial cells from healthy uteri collected at the abattoir. These uteri were classified into post-ovulatory, early-to-mid luteal, late luteal or pre-ovulatory phase (n=8 animals for each cycle phase). After collecting endometrial epithelium using the cytobrush-method, mRNA analysis was performed by real-time RT-PCR. L-PGDS, IL-1alpha and IL-1-RN mRNA were expressed significantly higher (P<0.05) in the endometrium of cows with subclinical or clinical endometritis compared with healthy cows. A twofold lower cPGES mRNA expression (P<0.05) was detected in cows with subclinical endometritis compared to healthy cows. L-PGDS and IL-1-RN mRNA expression was increased (P<0.05) after ovulation compared with the pre-ovulatory or luteal phase, respectively. These results support the hypothesis that a dys-regulated cytokine and/or prostaglandin profile in the uterus could be induced by subclinical endometritis or clinical endometritis.

Keywords Pubmed: Animals
Cattle
Cattle Diseases/metabolism*
Cytosol/enzymology
Endometritis/metabolism
Endometritis/veterinary*
Endometrium/chemistry*
Epithelium/chemistry
Estrous Cycle/physiology
Female
Gene Expression
Interleukin 1 Receptor Antagonist Protein/genetics
Interleukin-1alpha/genetics
Intramolecular Oxidoreductases/genetics
Lipocalins/genetics
Microsomes/enzymology
Postpartum Period/metabolism
Prostaglandins/biosynthesis*
Prostaglandins/genetics*
RNA, Messenger/analysis*
Reverse Transcriptase Polymerase Chain Reaction


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