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Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Originalarbeit

Jahr: 2011

AutorInnen: Paschinger, K; Razzazi-Fazeli, E; Furukawa, K; Wilson, IB

Titel: Presence of galactosylated core fucose on N-glycans in the planaria Dugesia japonica.

Quelle: J Mass Spectrom. 2011; 46(6):561-567

Autor/innen der Vetmeduni Vienna:

Razzazi-Fazeli Ebrahim

Beteiligte Vetmed-Organisationseinheiten

Planarial species are of especial interest to biologists due to the phenomenon of pluripotency and, in comparison to other developmental processes, it can be hypothesised that glycan-lectin interactions may play a role. In order to examine the N-glycans of one of these organisms, Dugesia japonica, peptide:N-glycosidase A was employed and the released glycans were subject to pyridylamination, HPLC and mass spectrometric analysis. A range of oligomannosidic glycans was observed with a trimethylated Man(5) GlcNAc(2) structure being the dominant species. Three glycans were also observed to contain deoxyhexose; in particular, a glycan with the composition Hex(4) HexNAc(2) Fuc(1) Me(2) was revealed by exoglycosidase digestion, in combination with MS/MS, to contain a galactosylated core α1,6-fucose residue, whereas this core modification was found to be capped with a methylhexose residue in the case of a Hex(5) HexNAc(2) Fuc(1) Me(3) structure. This is the first report of these types of structures in a platyhelminth and indicates that the "GalFuc" modification of N-glycans is not just restricted to molluscs and nematodes.

Keywords Pubmed: Animals
Carbohydrate Conformation
Chromatography, High Pressure Liquid
Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase/chemistry
Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase/metabolism
Tandem Mass Spectrometry

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