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Gewählte Publikation:

Publikationstyp: Zeitschriftenaufsatz
Dokumentart: Originalarbeit

Publikationsjahr: 2011

AutorInnen: Willmann, C; Budik, S; Walter, I; Aurich, C

Titel: Influences of treatment of early pregnant mares with the progestin altrenogest on embryonic development and gene expression in the endometrium and conceptus.

Quelle: Theriogenology. 2011; 76(1):61-73



Autor/innen der Vetmeduni Vienna:

Aurich Christine
Budik Sven
Walter Ingrid
Willmann Conrad

Beteiligte Vetmed-Organisationseinheiten
Graf Lehndorff Institut für Pferdewissenschaften
Institut für Topographische Anatomie
Plattform Besamung und Embryotransfer


Abstract:
A positive influence of altrenogest treatment on a retarded development of the conceptus around the beginning of placentation in mares older than 8 years could be recently demonstrated. In the present study, effects of altrenogest treatment in early-pregnant mares on conceptus development and expression of endometrial and embryonic genes were investigated. Genes were chosen according to a possible involvement in embryo-maternal interaction and embryonic development in the equine species. Mares were treated with altrenogest (0.044 mg/kg bodyweight) or sunflower oil (placebo) from day 5 to 11 after ovulation. Embryos (altrenogest n = 13, placebo n = 12) and biopsies were collected on day 11. Pregnancy rate and embryonic size were not influenced by treatment (embryonic diameter: altrenogest 7.0 ± 2.5, placebo 6.5 ± 1.7 mm, n.s.). The percentage of luminal epithelial cells, superficial glandular epithelial cells and interstitial cells with nuclei staining positively for the progesterone receptor was significantly lower (P < 0.05) in samples collected from altrenogest-treated than from placebo-treated mares (e.g., luminal epithelium: altrenogest 1.9 ± 1.7%, placebo 23.0 ± 10.5%, P < 0.05). Staining for COX2 (cyclooxygenase-2) was not affected by treatment. In the endometrium a slight but significant increase in the number of PMN (polymorph nuclear neutrophils) was seen in response to treatment (altrenogest 0.8 ± 0.5 PMN/field, placebo 0.3 ± 0.3 PMN/field; P < 0.05). No differences in the relative gene expression of COX2, the receptors for progesterone, estrogens and growth hormone as well as for IGF (insulin-like growth factor) 1 and 2 were detected. The relative gene expression of aquaporin 3 in relation to β-actin differed significantly (P < 0.05) between embryos from altrenogest (3.2 ± 0.8) and placebo-treated mares (1.3 ± 0.2), but no other genes were affected. The study demonstrates down-regulation of progesterone receptors in the endometrium of early pregnant mares by treatment with the progestin altrenogest. This increased expression of aquaporin 3 in the conceptus was not related to changes in embryonic size or development.

Keywords Pubmed: Animals
Embryo, Mammalian/cytology
Embryo, Mammalian/drug effects
Embryonic Development/drug effects*
Endometrium/cytology
Endometrium/drug effects*
Endometrium/metabolism
Female
Gene Expression Regulation, Developmental
Horses/embryology*
Horses/genetics
Pregnancy
Pregnancy Rate
Progesterone Congeners/pharmacology*
Trenbolone Acetate/analogs & derivatives*
Trenbolone Acetate/pharmacology
Trophoblasts/cytology
Trophoblasts/drug effects
Trophoblasts/metabolism


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