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Gewählte Publikation:

Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Originalarbeit

Jahr: 1998

AutorInnen: Rosenberg, E; Krska, R; Wissiack, R; Kmetov, V; Josephs, R; Razzazi, E; Grasserbauer, M

Titel: High-performance liquid chromatography-atmospheric-pressure chemical ionization mass spectrometry as a new tool for the determination of the mycotoxin zearalenone in food and feed.

Quelle: J Chromatogr A (819), 1-2 277-288.



Autor/innen der Vetmeduni Vienna:

Razzazi-Fazeli Ebrahim

Beteiligte Vetmed-Organisationseinheiten
Institut für Tierernährung und funktionelle Pflanzenstoffe


Abstract:
A new method for the determination of the mycotoxin zearalenone (ZON) in food and feed, based on HPLC-MS with an atmospheric-pressure chemical ionization (APCI) interface after extraction from cereals and clean-up by either conventional solid-phase or immunoaffinity cartridge is presented. The APCI interface parameters are optimized to provide detection of ZON with maximum sensitivity after RP separation of ZON on a C18 column with acetonitrile-water (40:60, v/v) at 1 ml/min column flow without split. Using APCI-MS detection, the sensitivity of the method was improved by a factor of ca. 50 in comparison to HPLC with fluorescence detection, allowing determination of ZON down to 0.12 microgram/kg maize which is well below present threshold values. Due to the selectivity of MS detection, it also was possible to quantitatively determine ZON both in raw extracts without clean-up using a normal-size (100 mm) chromatographic column or using only a short (20 mm) chromatographic column, when a clean-up was done to minimize possible interferences.

Keywords Pubmed: Animal Feed/analysis*
Atmospheric Pressure
Chromatography, High Pressure Liquid/methods*
Food Analysis/methods*
Ions
Mass Spectrometry/methods*
Reproducibility of Results
Temperature
Zearalenone/analysis*


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