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Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Originalarbeit

Jahr: 2012

AutorInnen: Paschinger, K; Hykollari, A; Razzazi-Fazeli, E; Greenwell, P; Leitsch, D; Walochnik, J; Wilson, IB

Titel: The N-glycans of Trichomonas vaginalis contain variable core and antennal modifications.

Quelle: Glycobiology. 2012; 22(2):300-313

Autor/innen der Vetmeduni Vienna:

Razzazi-Fazeli Ebrahim

Beteiligte Vetmed-Organisationseinheiten

Trichomonad species are widespread unicellular flagellated parasites of vertebrates which interact with their hosts through carbohydrate-lectin interactions. In the past, some data have been accumulated regarding their lipo(phospho)glycans, a major glycoconjugate on their cell surfaces; on the other hand, other than biosynthetic aspects, few details about their N-linked oligosaccharides are known. In this study, we present both mass spectrometric and high-performance liquid chromatography data about the N-glycans of different strains of Trichomonas vaginalis, a parasite of the human reproductive tract. The major structure in all strains examined is a truncated oligomannose form (Man(5)GlcNAc(2)) with α1,2-mannose residues, compatible with a previous bioinformatic examination of the glycogenomic potential of T. vaginalis. In addition, dependent on the strain, N-glycans modified by pentose residues, phosphate or phosphoethanolamine and terminal N-acetyllactosamine (Galβ1,4GlcNAc) units were found. The modification of N-glycans by N-acetyllactosamine in at least some strains is shared with the lipo(phospho)glycan and may represent a further interaction partner for host galectins, thereby playing a role in binding of the parasite to host epithelia. On the other hand, the variation in glycosylation between strains may be the result of genetic diversity within this species.

Keywords Pubmed: Amino Sugars/chemistry*
Chromatography, High Pressure Liquid/methods
Polysaccharides/isolation & purification
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods
Trichomonas vaginalis/chemistry*
Trichomonas vaginalis/metabolism

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