Introduction During the last years an increased interest has been observed in establishment of techniques for automatic lameness detection in dairy cows. One of these techniques is the accelerometry. Material and methods In 16 non-lame and clinically sound Simmental (Fleckvieh) cattle (< 6.5 years old), 3D-acceleration data were collected using an accelerometer fixed on the skin over the sacrum during walk. Following this baseline measurement, a 19 mm high plastic block was attached on a randomly selected inner hind claw in 14 out of 16 cows before locomotion was scored and measured again. The RMS (root mean square) value as well as mean minimum (gMin) and mean maximum (gMax) acceleration were evaluated and a forecast model for score 1 (non-lame) was calculated. Results The evaluation of the acceleration data of the x, y and z axes of non-lame cows revealed a regular and constant curve progression. Cows with the block attached showed a locomotion score of 1 (not lame). However, a hardly noticeable asymmetry at walk was found, although no flexion of the back could be noticed. There was a significant increase (p<0.05) in gMin in the vertical direction. Other parameters increased but were not statistically significant. The forecast model classified all 16 animals as sound (free of lameness). After attachment of the block, 11 out of 14 cows (78.6 %) were classified as sound again. Conclusion No significant differences were found for clinical lameness scoring and for the acceleration data of cows before and after attaching a block. The collected 3D-acceleration data of physiological movement in non-lame cows during walk provided a homogeneous set of data, which seems suitable for comparison with data of lame cows.