Veterinärmedizinische Universität Wien Forschungsinformationssystem VetDoc

Grafischer Link zur Startseite der Vetmeduni Vienna

Gewählte Publikation:

Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Originalarbeit

Jahr: 2012

AutorInnen: Tvarijonaviciute, A; Gutiérrez, AM; Miller, I; Razzazi-Fazeli, E; Tecles, F; Ceron, JJ

Titel: A proteomic analysis of serum from dogs before and after a controlled weight-loss program.

Quelle: Domest Anim Endocrinol. 2012; 43(4):271-277



Autor/innen der Vetmeduni Vienna:

Miller Ingrid
Razzazi-Fazeli Ebrahim

Beteiligte Vetmed-Organisationseinheiten
Institut für Medizinische Biochemie
VetCore


Abstract:
The objective of this study was to investigate how weight-loss program would alter the proteome of the serum of Beagle dogs. For this purpose, serum samples from 5 Beagle dogs, before and after weight loss, were analyzed using 2-dimensional electrophoresis. Protein profiles of all samples were obtained, divided into 2 classes (obese and lean), and compared using specific 2-dimensional software, giving a total of 144 spot matches. Statistical analysis revealed 3 spot matches whose expressions were modulated in response to weight loss: 2 protein spots were upregulated and 1 protein spot was downregulated in the obese state in comparison with the lean state of the dogs. Mass spectrometric identification of differentially regulated spots revealed that these protein spots corresponded to retinol-binding protein 4, clusterin precursor, and α-1 antitrypsin, respectively, which could be considered potential markers of obesity and obesity-related disease processes in dogs.

Keywords Pubmed: Animals
Biological Markers/blood
Blood Proteins/analysis*
Clusterin/blood
Dog Diseases/blood*
Dog Diseases/therapy*
Dogs
Electrophoresis, Gel, Two-Dimensional/veterinary
Female
Mass Spectrometry/veterinary
Obesity/blood
Obesity/therapy
Obesity/veterinary*
Protein Precursors/blood
Proteomics*
Retinol-Binding Proteins/analysis
Weight Loss/physiology*
alpha 1-Antitrypsin/blood


© Veterinärmedizinische Universität Wien Hilfe und DownloadsErklärung zur Barrierefreiheit